2
Installation
Installing the LightPipe Flowcell
18
Accela PDA Detector Hardware Manual
Thermo Scientific
3. Unscrew the retaining block knob and remove the retaining block (see
Figure 14.
View of the retaining block and the retaining block knob
4. Remove the protective end caps from the ends of the LightPipe flowcell.
5. Connect the inlet port of the LightPipe flowcell:
a. Use the PEEK fitting included with the LightPipe Flowcell Kit to connect one end of
the red 0.005 in. ID, insulated inlet tubing to the flowcell inlet port. Slide the
protective insulating sleeve of the tubing as close as possible to the inlet port of the
LightPipe flowcell.
b. Connect the other end of the tubing to the LC column outlet.
6. Connect the outlet port of the LightPipe flowcell:
• If you are not connecting to an MS detector, use a PEEK fitting to connect the blue
0.010 in. ID, outlet tubing to the LightPipe flowcell outlet. Place the other end of the
outlet tubing into the waste reservoir. When you use an optional backpressure
regulator (see
), connect the outlet line to the low-pressure union
and waste tubing.
• If you are connecting the LightPipe flowcell outlet to an MS detector, use the PEEK
fittings to connect one end of the red 0.005 in. ID, PEEK tubing (included in the
MS detector accessory kit) to the outlet port of the LightPipe flowcell and to connect
the other end of the tubing to the inlet port of the MS detector.
Note
The insulating sleeve of the inlet tubing minimizes temperature
fluctuations, which cause baseline drift.
1
Retaining
block knob
Retaining
bolt
Retaining block