Teledyne Photometrics KINETIX Скачать руководство пользователя страница 20

 

11 

 

58-723-004 Rev A01 

Chapter 3 – Theory of Operation 

 

 
Introduction 

 

 
 

Backside-illuminated scientific CMOS (BSI Scientific CMOS) sensors are a recent but rapidly 
maturing development in image sensor technology. Boasting near-perfect 95% quantum 
efficiency (QE), they are able to provide the highest levels of sensitivity. New cameras can 
now leverage these high-QE sensors to offer a combination of low read noise, high full well 
capacity, and fast readout rates that is ideal for modern low-light imaging. 

 

 
CMOS Image 
Sensor 
Structure 

 

 
 
A major difference between traditional CCD sensors and CMOS sensors is the location where 
charge-to-voltage conversion of accumulated photoelectrons takes place.  
 
CCD sensors transfer the pixels’ accumulated signal in charge packets in “bucket brigade” 
fashion across the sensor to a common output node where charge is converted to a voltage. 
The voltage is then sampled using off-chip analog-to-digital converters (ADCs) and 
transferred to the PC as digital grey values. 
 
While providing excellent quantitative photometry and very high image quality, the large 
number of transfers and sequential digitization of pixels results in low frame rates. This 
speed penalty increases with the number of pixels to be digitized. 
 
CMOS sensors leverage many of the same analog signal concepts used in CCDs but place 
the output node circuitry inside each pixel. This eliminates the charge transfer process. 
To read the signal from a given row, the accumulated charge is converted to a voltage 
inside the pixel, then each pixel in the row is connected to the appropriate column 
voltage bus, where the on-chip ADCs convert the voltages to a grey value. 
 

The parallel digitization of all pixels in a row provides CMOS devices with a tremendous 
speed advantage. Imagine a CCD with 3200 x 3200 pixels – and each pixel’s voltage is 
measured in 1 µsec. To read a single row, 3200 voltage measurements are performed in 
serial fashion taking slightly longer than 3.2 msec, and when repeated for 3200 rows, the 
entire image takes more than 10 seconds to be digitized.

 

 

On a CMOS device, the entire 3200 voltage conversions needed to digitize a row happen in 
parallel. If the time to digitize a pixel remains at 1 µsec, the time to read the entire frame is 
now 3.2 msec. In practice, the time saving is split between faster frame rates and slowing 
the rate of pixel measurement to reduce electronic noise. For example, if the time to 
measure a pixel was increased to 3.749 µsec to reduce noise, then the image sensor can 
still be read in 12 msec (for a maximum 83 fps). 
 

Содержание KINETIX

Страница 1: ...i...

Страница 2: ...contained herein is subject to change without notice Revision of this publication may be issued to incorporate such change LIMITED WARRANTY Teledyne Photometrics Teledyne Photometrics us we our makes...

Страница 3: ...ree version upgrades Version upgrades supplied free of charge will be in the form of a download from the Internet Those customers who do not have access to the Internet may obtain the version upgrades...

Страница 4: ...ne Photometrics liability exceed the cost of the repair or replacement of the defective product or part This limited warranty gives you specific legal rights and you may also have other rights that ma...

Страница 5: ...v 58 723 004 Rev A01...

Страница 6: ...vi 58 723 004 Rev A01...

Страница 7: ...w 1 About This Manual 1 Precautions 1 Environmental Requirements 2 Storage Requirements 2 Microscopes Lenses and Tripods 2 Repairs 2 Cleaning 2 Chapter 2 System Installation 3 Introduction 3 Getting t...

Страница 8: ...Width 21 Scan Direction 21 Device Synchronization Triggering 22 Trigger Modes 23 Internal Mode 23 Trigger First Mode 23 Edge Mode 23 Level Trigger Mode 23 Level Trigger Overlap Mode 23 Usage Summary...

Страница 9: ...Not Displayed 30 Camera Running Too Warm 30 PVCAM Error Message Appears 31 Lengthy Pauses During Imaging 31 Chapter 6 Basic Specifications 32 Kinetix Dimensional Drawings 32 Camera Weight 32 Sensor Sp...

Страница 10: ...for Kinetix system components Precautions The CMOS sensor and other system electronics are extremely sensitive to electrostatic discharge ESD To avoid permanently damaging the system please observe th...

Страница 11: ...mount adapter The camera also allows you to install any lens that is compatible with the standard threaded mounts as long as its optics do not extend behind the flange of the adapter Kinetix can be mo...

Страница 12: ...power supply with international power cord set I O to 8 BNC trigger breakout cable part number CBL IO 8 BNC USB memory device containing PVCAM library and drivers Quick Installation Guide PCI Express...

Страница 13: ...g up Power Switch Turns the camera on and off Trigger Hirose HR10A 10R 10S for use with Teledyne Photometrics trigger to BNC breakout cable CBL IO 8 BNC Power in Connection to external 12V 10A DC powe...

Страница 14: ...s based computers Additional instructions are included for installing a PCI Express interface card in the computer The Teledyne Photometrics USB memory device contains the following files Manuals Dire...

Страница 15: ...the one shown in the photo Before attempting to operate the camera first install this interface card into the PC via the following steps 1 Shut down the PC 2 Unplug the PC from power mains and ensure...

Страница 16: ...tion on the side cover 5 Hold the PCIe card being careful not to touch the board components or PCIe bridge pins and insert it properly orientated into the open slot The card should slide into place wi...

Страница 17: ...s when correctly oriented do not force the connectors If a connector does not insert simply turn the connector over and retry To ensure proper system performance one of the cables should be used to co...

Страница 18: ...er on the computer and let it go through its normal startup procedure 5 Turn on Kinetix and allow it 30 seconds to boot before starting software For Kinetix builds June 25 2021 or later camera serial...

Страница 19: ...to drop frame rate For this reason Teledyne Photometrics recommends isolating the camera to its own USB 3 2 root hub as shown in the Windows Device Manager A method for creating an independent root hu...

Страница 20: ...peed penalty increases with the number of pixels to be digitized CMOS sensors leverage many of the same analog signal concepts used in CCDs but place the output node circuitry inside each pixel This e...

Страница 21: ...ents into a single 16 bit value is the function of the digital gain combiner This mathematical operation is performed on the camera s FPGA The result is a single gain of approximately 0 25 e ADU In pr...

Страница 22: ...nsitivity Mode Sub Electron Mode Line Time sec line 3 75 0 625 3 53125 60 1 Line times for the various modes of the Kinetix camera The graphic below depicts the time delay between each row of pixels i...

Страница 23: ...reaching the sensor as well as thermally generated sensor dark current To eliminate this pre acquisition charge accumulation most CCD and CMOS cameras clear the sensor one or more times prior to expos...

Страница 24: ...and the corrections are stored in the camera The corrections are then applied in real time to each image The dynamic fluctuations must be detected and corrected with each acquisition The Kinetix came...

Страница 25: ...e local median A Despeckle Bright High threshold of 120 indicates that a pixel that is 20 brighter than the local median will be replaced The intensity range in which each filter operates can be set b...

Страница 26: ...right Low block any light from reaching the sensor and examine the bias histogram This allows viewing of the histogram s tail where the effect of the filters can be seen Adjust the filters to trim the...

Страница 27: ...ise The Speed 8 bit Mode provides the highest frame rates The Sub Electron 16 bit Mode uses two approaches to generate data similar to that generated by EMCCD sensors but without the complications of...

Страница 28: ...erest that rolls across the columns of the sensor using the rolling shutter readout to build the final image Should the region of interest coincide with the focus of illumination light as in certain t...

Страница 29: ...ScanLineDelay Delay is added in increments of line time 2 line times for Kinetix non DR modes In this mode scan width becomes read only Effective line time using non 16 bit DR is calculated as 2 wher...

Страница 30: ...in sec for use in PSM calculations Type of Readout 16 bit Dynamic Range 12 bit Sensitivity 8 bit Speed 16 bit Sub Electron Single line 3 75 sec Dual line 3 53125 sec As of firmware version 30 29 03 n...

Страница 31: ...ith external hardware devices A 10 pin Hirose HR212 10RC 10SDL 74 connector is located on the back of the camera for trigger input output operations The signals provided to the user are shown in the c...

Страница 32: ...e out behavior and sequence size are set in the software application prior to acquiring the sequence Edge Mode Like Trigger First Mode Edge Mode requires a hardware trigger but for every frame The ris...

Страница 33: ...es down the columns Note The Kinetix camera s software trigger modes are essentially identical to the hardware trigger modes except for the trigger input In software trigger modes the trigger input wi...

Страница 34: ...l light sources in a way such that the signal detected at the camera starts and finishes at the same time across all rows This provides a Pseudo Global Shutter imaging method where all rows of the sen...

Страница 35: ...ger modes The Expose Out I O signal leaving the camera is high when any row in a single frame is exposing The length of the Expose Out signal is equal to the time between the start of the first row s...

Страница 36: ...me of each output independently This is much faster than using software based methods to control the timing of illumination devices Fan Speed Control Fans are often used to remove heat from the camera...

Страница 37: ...ed with each frame Metadata contains useful information like the exposure time ROI location and time stamps Metadata is inserted directly into the frame buffer and transferred together with the image...

Страница 38: ...portant that an individual cable be connected to the same port number on the camera as on the PCIe board Cross connecting such as when a cable connects port 1 on the camera to port 2 on the computer c...

Страница 39: ...ter has booted power cycle the camera 5 When the LED stops blinking restart the computer If the New Hardware Found dialog box still does not appear contact Teledyne Photometrics Customer Service Image...

Страница 40: ...e message s numerical code and then contact Teledyne Photometrics Customer Service Lengthy Pauses During Imaging If you notice lengthy pauses marked by a lot of disk activity while imaging Close any o...

Страница 41: ...32 58 723 004 Rev A01 Chapter 6 Basic Specifications Kinetix Dimensional Drawings Camera Weight All measurements given in millimeters 4 lbs 1 8 kg...

Страница 42: ...c CMOS Resolution 3200 x 3200 pixel array Pixel Size 6 5 m x 6 5 m Power Supply Specifications Voltage Input 100 240 V 50 60 Hz Current Input 2 5 A 110 V nominal Voltage Output 12 V 10 A Maximum Power...

Страница 43: ...a In Camera Out Circulator In Listen for the click 6 Pull each connector to ensure they are locked 7 Inspect the setup to ensure the hose connectors are secure at the cooler and camera 8 Set both the...

Страница 44: ...on Olympus and Zeiss Contact Teledyne Photometrics Customer Service if this does not work with your computer To start the camera after the computer boots up 1 Ensure the computer and camera are both p...

Страница 45: ...eved have been highlighted in green Note Teledyne Photometrics highly recommends using a 16x PCIe card slot on the motherboard for multi camera setups in order to achieve maximal data throughput Max F...

Страница 46: ...omes with Kinetix is compatible with active fiberoptic PCIe cables measuring up to 100 meters in length No special configuration of the card needs to be performed for working with these cables Maximum...

Страница 47: ...38 58 723 004 Rev A01 Notes...

Страница 48: ...39 58 723 004 Rev A01...

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