ENGLISH
ENGLISH
Page 24
Instruction manual 50100XXX Version 3 November-07
You should lower the stage according to the appropri-
ate working distance for the objective with lesser magni-
fication before placing the sample on it. Once you have
done this, you should place the slide by looking directly
at it (not by the eyepiece) and placing the sample centred
on the stage opening.
Looking through the eyepiece using the objective with
lesser magnification you should now raise the stage by
using the coarse knob until the sample appears. Then,
you should move the fine knob until the image is focused
(picture 6).
IMPORTANT:
You should always start operating by using the objective with lesser
magnification, as it facilitates focusing and makes impossible to ruin the slides or get the
objectives dirty. Furthermore, it is essential to adjust the previous lighting to using the
higher magnification, and it helps you to see the whole topographic structure of the sam-
ple so as you can concentrate on the more appealing parts to observe them at higher mag-
nifications.
By turning the nosepiece you will pass to an objective with higher magnification. As
they are paracentric you will only have to adjust focusing with the fine adjustment knob.
Adjust the condenser's height as to obtain a uniform field of view (the higher the mag-
nification is, the lower the field will be). Thus, condenser's adjustment will be more pre-
cise as the magnification value increases: when working with 40 magnifications (4x objec-
tive and 120x eyepiece) you should lower the condenser to cover a wide field and avoid
too much lighting intensity, raising it in a progressive way with higher magnification
objectives in order to gather the light beam and gain contrast and illumination. With the
oil immersion objective you should play with the condenser's height to focus properly.
Iris diaphragm enables decreasing the numerical aperture to a value similar to objec-
tive's. Thus you avoid marginal illumination that decreases contrast and therefore it will
be more closed when you use a lesser magnification objective and with a small numerical
aperture. You will only use its total aperture when you use the oil immersion objective.
In order to properly adjust the microscope you should start using the maximum aper-
ture. As contrast increases diffraction phenomena appear, limiting resolution because the
diaphragm is more closed. You will obtain the adequate adjustment value when you reach
the maximum contrast without distorting resolution.
A useful way of making the adjustment is the following one:
Take off the eyepiece and observe the objective's exit
pupil through the empty tube adjusting the diaphragm
aperture. Normally it is convenient to maintain the
diaphragm aperture on approx. 70-80% of the objective's
numerical aperture. (pic.7)
Closing the diaphragm by reducing the optical sys-
tem's numerical aperture increases the focusing depth.
You must not surpass the diffraction limit as an excuse to
increase the focusing depth. Diaphragm must not be
used to reduce luminous diffraction.
Picture 6
Pupila de salida
del objetivo
Apertura del
diafragma Iris
70%-80%
30%-20%
Picture 7
Version 3 November-07
Instruction manual 50100XXX Page 25
Security
The microscope must be used by previously qualified staff that knows how it works
thanks to the user manual.
You should put the microscope in a horizontal plane stable table, having a safety area
of at least 30 cm per side.
Do not place the microscope near any warm supply (burners, blowlamps, etc), nor
expose it directly to the sun. Avoid vibrations, dust and dry environments.
During its functioning dangerous materials such as flammable or pathological substan-
ces must be out of the safety area.
When you are not using the microscope for a long period of time please make sure it is
unplugged in order to avoid possible accidents.
It is essential to have the equipment switched off and unplugged from the net before
cleaning, checking components or replacing any piece (e.g. replacement of a fuse).
Never try to repair the microscope by yourself, since you will lose the warranty and
may provoke damages to the general operating system or the electrical installation, as
well as injuries to the people that usually handle the equipment (burns, hurts…).
Made under the European regulations for electrical security, electromagnetic compati-
bility and security on machines.
5. MAINTENANCE AND CLEANING
To get the best results and a higher duration of this equipment it is essential to follow
the processes of use.
Note:
All the processes of use mentioned below will not have any value unless you keep
a continued and careful maintenance.
Please follow the processes of use of this manual.
This manual should be available for all users of this equipment.
Always use original components and supplies. Other devices can be similar but they
can damage the equipment.
The microscope is supplied with a Schuko standard wire and it should be connected to
a current wire provided with an earth wire, it should be handy to be disconnected in case
of emergency.
Never try to repair the microscope by yourself, since you will lose the warranty and
may provoke damages to the general operating system or the electrical installation, as
well as injuries to the people that usually handle the microscope (burns, hurts…) or dama-
ges in nearby equipments.
In the event of breakdown please contact your distributor to overhaul through Ura
Technic Technical Assistance Department.
In case the lamp blows you should replace it for another one, making sure it is an Ura
Technic halogen lamp 6V 20W original. Please take care and do not touch the lamp with
bare hands.
