Techne Touchgene Gradient, Руководство оператора

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T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L
PAGE 26
Gradient Calculator
Temperature 55
°c Gradient 10°c
Gradient
Temperature
LHS Centre of Block RHS
60°C Highest temperature
55°C Set
temperature
50°C Lowest temperature
1 2 3 4 5 6 7 8 9 10 11 12
Columns
Temperature
Gradient
10°C
Print Exit
50.6 52.0 53.7 55.2 57.2 58.8
51.2 52.9 54.6 56.2 58.0 59.2
The Gradient
The Set Temperature is the temperature that you require of the MIDDLE ROWS of samples in the block.
The GRADIENT is the variation of the two extremes of the samples in the block; the left-hand side
being the coolest and the right-hand side being the hottest.
The Maximum gradient is 30°C; the lowest cold temperature is 20°C and the hottest temperature is
70°C. However, you must remember that the greater the gradient the poorer the uniformity.
The illustration shows a
Set Temperature of 55°C with a Gradient of 10°C on a 0.2ml x 40 block.
Other examples: Set temperature of 55°C with a gradient of 20°C will give a low of 45°C and a high of
65°C; a set temperatureof 50°C with a gradient of 16°C will give a low of 42°C and a high of 58°C.
Gradient Calculator
While the gradient is substantially linear there are small variations. We have, therefore, included a
gradient calculator to give you a more accurate picture of the gradient.
Touch the ‘Gradient Calculator’ button in “System User Main Menu”, see page 16, and you will go
into the “Gradient Calculator” screen.
An example is shown here but
please note that this is an
idealised picture for this
manual and you must use the
unit screen to get the accurate
picture for your block.