2013-06
Quick Guide NanoQuant Plate
TM
No.30035094 Rev No. 1.4
27
Infinite F200 PRO/Infinite F500
Abs
blank A1
= Abs
260 A1
– Abs
340 A1
[OD]
Abs
blank A2
= Abs
260 A2
– Abs
340 A2
[OD]
Abs
blank B1
= Abs
260 B1
– Abs
340 B1
[OD]
etc.
Individual blanking information is stored for each well used and blank-
correction of the samples is done with the corresponding single blanking
values instead of one average blank. Every well that is to be used for
sample measurement needs to be blanked beforehand.
4.3
Samples
Calculations based on Average Blanking
The well-specific absorbance value at 310 nm for Infinite M200 PRO and
Infinite M1000 (340 nm for Infinite F200 PRO and Infinite F500
) is
subtracted from the corresponding absorbance value at 260 nm. Each well
used for sample measurement is then blanked with the average blanking
value.
Infinite M200 PRO/Infinite M1000
Abs
A1
= (Abs
260 A1
– Abs
310 A1
)
– Abs
blank average
[OD]
Abs
A2
= (Abs
260 A2
– Abs
310 A2
)
– Abs
blank average
[OD]
Abs
B1
= (Abs
260 B1
– Abs
310 B1
)
– Abs
blank average
[OD]
etc.
Infinite F200 PRO/Infinite F500
Abs
A1
= (Abs
260 A1
– Abs
340 A1
)
– Abs
blank average
[OD]
Abs
A2
= (Abs
260 A2
– Abs
340 A2
)
– Abs
blank average
[OD]
Abs
B1
= (Abs
260 B1
– Abs
340 B1
)
– Abs
blank average
[OD]
etc.
