2013-06
Quick Guide NanoQuant Plate
TM
No.30035094 Rev No. 1.4
9
Figure 2: Correct orientation of NanoQuant Plate in the reader
11. The first measurement step is blanking with the required buffer. A
separate window opens and the blanking procedure can be observed.
12. The blanking measurement is started and can be monitored in the
measurement progress window. If blanking has been performed
successfully, the sample positions are highlighted in yellow and the
screen color changes to a homogenous green (Nucleic Acid
Quantification) or blue (Labeling Efficiency). Blanking results (date and
time, samples positions that were selected for blanking, blanking
range
, and maximum CV) are displayed next to the plate preview in
the measurement stripe and saved until the instrument is
disconnected.
13. When the blanking measurement has been completed successfully,
the plate is moved out automatically. The plate is now ready for
sample application and analysis. The green Start button is now
accessible.
14. Remove any remaining blanking buffer from the sample positions by
wiping the quartz spots with a piece of lint free paper and apply 2
μ
l of
the samples onto each spot.
15. When the NQP is loaded with samples and correctly placed onto the
plate carrier, click the green Start button.
