12
Quick Guide NanoQuant Plate
TM
No.30035094 Rev No. 1.4
2013-06
Additional blanking information
The blanking information will be stored until:
•
The reader is disconnected
•
The i-control is shut down
•
Another sample type is selected
If other sample types are selected, the screen color will become a light
green or light blue gradient again depending on the application and the
blanking procedure must be repeated with corresponding wavelengths.
If you have chosen a labeling efficiency measurement before starting a
blanking procedure, the type of samples and the specific wavelengths for
dyes have to be selected. If the correct fluorophores are selected, the
samples can be applied into the NanoQuant Plate.
Individual Blanking and Average Blanking
The user may choose between two distinct blanking options: Average
Blanking and Individual Blanking
(set by default)
. For Average Blanking,
choose which wells are to be used for blanking by dragging a frame around
the respective sample positions in the plate preview. It is generally
recommended to perform the blanking measurement with all 16 sample
positions, however average blanking must be done with at least two wells,
in order to calculate an average value that is used to blank-correct all
measured samples.
The OD results measured with average blanking have to be below 10% CV
in order to grant reliable measurement results.
Figure 5: Click Start Blanking button for Average Blanking
