Operating Manual
M01534_v1.1 MagicPrep NGS Instrument Operating Manual
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4 Description of Function
This chapter explains the basic functions of the MagicPrep NGS instrument.
4.1
Overview
The MagicPrep NGS instrument and associated reagent kits provide an automated, hands-free
method of library preparation for NGS sequencing. The workflow starts with end repair of fragmented
DNA or enzymatic fragmentation of genomic DNA followed by adapter ligation, PCR amplification and
bead purification to produce the final library.
Samples of DNA or RNA along with magnetic beads are placed onto a sample deck which is inserted
into the MagicPrep NGS instrument. This sample deck contains the bulk reagents, pipette tips, and
several rows of PCR tubes which may contain additional reagents. A reagent cartridge specific for the
library preparation method is also inserted into the MagicPrep NGS instrument. This contains the
reagents specific for the library preparation method. Samples are manipulated fully automatically in
the MagicPrep NGS instrument using conventional technology which includes an 8-channel pipetting
engine with disposable tips, magnetic bead separation using a conventional magnet system and PCR
using a thermal cycler.
The operator is not able to access the internal parts of the MagicPrep NGS at any time. Insertion and
removal of the reagent cartridge and sample deck are the only manual interactions with the
instrument.
The main components of the MagicPrep NGS system are shown below:
Clockwise from the top: MagicPrep NGS instrument, the assembled Sample Deck (with bench stand)
and the Reagent Cartridge
