6
TAKARA BIO INC.
CycleavePCR
TM
O157 (VT1/VT2) Detection Kit Ver.2.0
v.0607
Cat.#CY203
URL:http://www.takara-bio.com
(3) Set the graphs. (Since the created graphs are saved, no entry is required in subsequent
reactions).
(3)-1. Set the amplification curve (FAM) for the amplified product derived from VT1.
Click the icon “Define Graphs” and create the graphs by following the steps shown
below.
(Since the graphs have been set under a name “FAM” at initialization, no entry is
required here).
(3)-2. Set the amplification curve (TET) for the amplified product derived from Internal.
Click the icon “Define Graphs” and create the graphs by following the steps shown
below.
(Since the graphs have been set under a name “TET” at initialization, no entry is
required here).
(3)-3. Set the amplification curve (ROX) for the amplified product derived from VT2.
Click the icon “Define Graphs” and create the graphs by following the steps shown
below.
(Since the graphs have been set under a name “ROX” at initialization, no entry is
required here).