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TAKARA BIO INC.
CycleavePCR
TM
O157 (VT1/VT2) Detection Kit Ver.2.0
v.0607
Cat.#CY203
URL:http://www.takara-bio.com
(3) The background appears to be high, because the weak fluorescent signal has been
detected and the Y-axis scale on the graph has been automatically adjusted to the
value for the detected signal.
→
Adjust the Y-axis scale manually.*
1.
Click any point near the Y-axis scale with
a right mouse button to open the Axes
Graph Scale screen.
2.
Enter an appropriate value (for example, the
value along the Y-axis in the positive
control reaction) in the Max field in the Y-
Axis (F1.) and click the “Apply” button.
3.
Click the “Exit” button to close the Axes
Graph Scale screen.
* During reaction, no scale can be adjusted. Scale adjustment must be done after all
the reactions are terminated.
Y-axis value adjusted according to
fluorescent signal value
After the Y-axis Max value was
manually adjusted to 500
VIII. Reference:
1.Takao,T., T.Tanabe, Y.-M.Hong, Y.Shimonishi, H.Kurazono, T.Yutsudo, C.Sasakawa,
M.Yoshikawa and Y.Takeda:(1988). Identity of molecular structure of Shiga-like
toxin(VT1)from
Esherichia coli
O157: H7 with that of Shiga toxin,
Microb.Pathog
., 5: 357-369
2.Jackson,M.P., R.J.Neil, A.D.O’Brien, R.K.Holmes and J.W.Newland(1987). Nucleotide
sequence analysis and comparison of the structural gene for Shiga-like toxin Öü and
Shiga-like toxin II encoded by bacteriophages from
Escherichia coli
933,
FEMS
Microbio.Lett
., 44:109-114
3.Ito,H.,A.Terai,H.Kurokawa,Y.Takeda and M.Nishibuchi:(1990).Cloning and nucleotide
sequencing of Vero toxin 2 variant genes from
Escherichia coli
O91:H21 isolated from a
patient with the haemolytic uremic syndrome,
Microb.Pathog
.,8:47-60
4.Weinstein,D.L.,M.P.Jackson,J.E.Samuel,R.K.Holmes and A.D. O’Brien: (1955). Cloning and
Sequencing of a Shiga-like toxin typeII variant from a
Escherichia coli
strain responsible for
edema disease of swine,
J.Bacteriol
., 170:4223-4230