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TAKARA BIO INC.
URL:http://www.takara-bio.com
CycleavePCR
TM
O157 (VT1/VT2) Detection Kit Ver.2.0
v.0607
Cat.#CY203
(6) After the reaction process terminates, click “Results Table” for viewing. Look at data in
the FAM Std/Res (Standard/Results) column for verifying the results. If the fluorescent
signal value for amplified product derived from the VT1 gene is 100 or larger, “POS” is
displayed in the cell of the " FAM Std/Res ". If it is smaller than 100, “NEG” is displayed.
Similarly, the results derived from VT2 gene appear in the " TxR Std/Res " column, and the
results derived from Internal Control appear in the " TET Std/Res ".
The result of VT1 gene detection is displayed.
The result of VT2 gene detection is displayed.
The result of internal control detection is displayed.
VI-5. Judgement
If the fluorescent signal value for FAM detection is 100 or larger in the 50-cycle real time PCR
process, “POS” is displayed in the cell of the FAM Std/Res (the results of detection of the
fluorescent signal derived from VT1 gene) in the Results Table, while if it is smaller than
“100”, NEG is displayed.
If the fluorescent signal value for ROX detection is 100 or larger, "POS" is displayed in the cell
of ROX Std/Res ( the detection result of the fluorescent signal derived from VT2 gene) in the
Results Table, while NEG is displayed if it is smaller than 100.
TET Std/Res (the results of detection of the fluorescent signal derived from Internal Control)
are also displayed in the same manner as those for FAM. Based on the results, judgement
should be made with the reference table for judgement shown in page 15.
(NOTE) In some cases, “POS” may appear in the cell of the FAM Std/Res, TET Std/
Res, or ROX Std/Res column accidentally due to the signal noises other than
those from the amplified product. The shape of the amplified curve must be
checked to see if a sigmoid curve has been drawn allow to correct determina
tion.
→
See also the section “Trouble shooting” (in page 19).