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Contents

Section 1: Main Components……………………………..3

Stretch Unit……………………………………………...3
Control Unit Front Panel……………………………….4
Control Unit Back Panel……………………………….4
Silicone Strain Chamber……………………………….5

Section 2: Use of the Cell Strain Instrument…………..6

Preparation of Cell Strain Instrument………………...6
System Operation………………………………………6
Culturing Cells in the Silicone Chambers…...……….7
Preparation of Silicone Chambers…...……………….8

Section 3: Strain Parameters………………………........10
Section 4: FAQ……………………………………………...11

Section 5: References

……………………………………

..13

Section

Safety

Instructions

and Precautions

……

...15

Section

Warranty

………………………………………

..1

Содержание STB-140-10

Страница 1: ...Cell Stretching System Model STB 140 10 User Manual Strex US Office 100600 Carroll Canyon Rd Suite 100 San Diego CA 92131 Email info strexcell com www strexcell com ...

Страница 2: ...er 5 Section 2 Use of the Cell Strain Instrument 6 Preparation of Cell Strain Instrument 6 System Operation 6 Culturing Cells in the Silicone Chambers 7 Preparation of Silicone Chambers 8 Section 3 Strain Parameters 10 Section 4 FAQ 11 Section 5 References 13 Section 6 Safety Instructions and Precautions 15 Section 7 Warranty 19 ...

Страница 3: ...er Length Adjusting Knob Use the knob to adjust the distance of chamber brackets to maintain tension on the chamber The bottom of the chamber should be taut Connectors Use Connector Cable to connect Control Unit to Stretch Unit Alignment Pins Holds Strain Chamber Brackets in place Chamber Brackets Each chamber is mounted on four pins two pins on each bracket Mounting Stage Brackets sit on top of t...

Страница 4: ...to increase and decrease stretch ratio Strain Frequency Selector Use upper and lower buttons to adjust cycle frequency Water Pump Switch Manual Override Use to turn on and off the pump that circulates water to cool the motor Note Water Pump will automatically turn on when temperature sensors reach over 40 C System Coolant Inlet Outlet Valves Be sure to supply cool water when in operation Failure t...

Страница 5: ...42mm 10mm 5 29mm 4 places for hooks holes 51mm 35mm Silicone Strain Chambers ST CH 10 Chamber Dimensions 32mm x 32mm x 10mm ...

Страница 6: ...1 To release the metal plugs from the Stretch Unit push backward on the outer metal rings around the valves The single larger black plug supplies electricity to the Stretch Unit this plug has to be in the correct orientation to fit properly Improper orientation will break the outlet The other end of the Connector Cable has 2 exposed latex tubes which fit onto the System Coolant Inlet and Outlet Va...

Страница 7: ...tch cycle may damage the motors 4 Push the Start Button to start the stretch unit 5 After 5 minutes stop the stretching and check the condition of the cells If the cells have not detached proceed with your experiment If the cells are detached the adhesion matrix coating was probably insufficient Recoat the chambers Culturing Cells in the Silicone Chambers 1 Seed cells at the appropriate concentrat...

Страница 8: ...erile silicone chambers in a Petri dish in preparation for coating Fibronectin Coating Preparation of Fibronectin solution 1 Dilute human or bovine Fibronectin to a final concentration of 50 to 100 ug ml in Phosphate Buffered Saline PBS Coating with Fibronectin solution 1 Pour 3 ml of the Fibronectin solution into each strain chamber 2 Incubate at 37oC for more than 30 minutes 3 Aspirate the fibro...

Страница 9: ...e Coating with collagen solution 1 Coat chamber with a thin layer 2 Aspirate excess 3 Dry in biological safety cabinet at 25 C or below The chamber can be stored at the same temperature 4 Wash the chamber twice with culture medium 5 If coating is successful water will not be repelled The PDMS silicone chambers are very hydrophobic with two methyl bases on the surface Cells adhere to the fibronecti...

Страница 10: ... 5 Hz Sine wave Stretch 1 sec contract 1 sec repeat 2 15 cycles minute 0 25 Hz Sine wave Stretch 2 sec contract 2 sec repeat 3 10 cycles minute Sine wave Stretch 3 sec contract 3 sec repeat 4 20 cycles minute Square wave Hold 0 5 sec stretch 1 sec hold 0 5 sec contract 1 sec repeat 5 10 cycles minute Square wave Hold 2 sec stretch 1 sec hold 2 sec contract 1 sec repeat 6 1 cycle minute Square wave...

Страница 11: ...ing your cell suspension in the chamber Q3 Cell attachment on the stretch chamber was confirmed by microscopy But the cells detached from the chamber surface after stretching the cells A3 Try seeding your chambers at a lower concentration of cells Over confluent cells generally adhere to neighboring cells rather than to the base matrix dish surface When an excess amount of cells are put in a cultu...

Страница 12: ...n Wash the cells once with PBS Add cell extract buffer directly into the chamber and collect the cell extract by using a cell scraper 3 RNA Wash the cells once with PBS for RNA preparation Add RNA extraction buffer directly into the chamber and collect the cell extract by using a cell scraper Q6 I want to use recombinant cells for an experiment A6 Direct transfection of cells in the strain chamber...

Страница 13: ...5 619 1998 8 Pp125FAK is required for stretch dependent morphological response of endothelial cells K Naruse T Yamada X Sai M Hamaguchi and M Sokabe Oncogene 17 455 463 1998 9 Orientation Change of Cardiocytes Induced by Cyclic Stretch Stimulation Time Dependency and Involvement of Protein Kinases K Kada K Yasui K Naruse and J Toyama J Mol Cell Cardio 31 247 259 1999 10 Molecular Identification of...

Страница 14: ...nel bymembrane stress crated with amphipaths Mol Membr Biol 22 6 519 527 2005 21 Stretch induced cell proliferation is mediated by FAK MAPK pathway Life Sci 76 24 2817 2825 2005 22 Fabrication of reconfiguration protein matricesby cracking X Zhu K Mills P Peters J Bahng El Liu J Shim K Naruse M Csete M Thouless S Takayama Nature Materials 4 403 406 2005 23 Involvement of reactive oxygen species in...

Страница 15: ...nnect gently by holding its plug not the cable Do not plug many objects into a single electrical outlet since it may cause fire If you are using an extension cord ensure it can withstand the total current to be used Disconnect power from the unit when it is not in use Connect the instrument to a power surge protected outlet Installation Location and Environment Keep the instrument on a stable leve...

Страница 16: ... Please refrain from modifying the machine without our permission you may be shocked or injured If you do attempt to modify the machine the warranty on the unit is void and we will not be responsible for any performance deterioration or unit malfunction In the case of any abnormal sound smell or smoke disconnect the power immediately and contact Strex Do not run the machine overloaded Be cautious ...

Страница 17: ...ck or equipment malfunction Please do not use under abnormal conditions If the machine is overheating emitting a strange odor etc disconnect the power cable from the outlet immediately Failure to do so may result in a fire or electric shock Do not use voltage other than the indicated power supply voltage Failure to do so may result in fire or electric shock Be sure to use the supplied power cable ...

Страница 18: ...ly inserted it may cause electric shock or fire Do not place heavy objects on top of this machine If you place heavy objects on the machine the items may collapse or fall and cause injury Usage Notice Periodically clean the plug and receptacle once a month and check that it is securely inserted When you are not using the machine for a long time please be sure to unplug the power cable from the out...

Страница 19: ...age resulting from an attempted repair or modification of the instrument by the user Damage caused by unavoidable external causes such as earthquakes lightening fire flood gas leak power surges or other acts of providence 3 Strex is free from any responsibility for effects or loss or damages arising from the result of the machine operation This warranty assures that Strex will repair our product f...

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