6
STEINDORFF
®
1)
Optical part
PROBLEM
CAUSE
SOLUTION
The edge of the
field of view has
shadow or the
brightness is
asymmetry
The nosepiece is not in the located
position (objective is not in the center
of the optical path)
Turn objective to the right
position(the optical light
center)
The image of the filament is not in the
center
Center it
The surface of the lens is moldy or
has contaminant (include condenser,
objective, eyepiece and collector)
Clean it up
Find dust and stain in
the field of view
There are stains on the lens (include
condenser, objective, eyepiece and
collector)
Clean it up
There are stains on the specimen
Clean it up
The position of the condenser is too
low
Raise it to proper position
The image is
defocused (low
resolution \ contrast)
There is no coverslip on the
specimen
Add coverslip
The coverslip is too thick or too thin
Use the standard coverslip
(0.17mm )
The specimen is placed inversely
Reverse it back
There was oil on the dry objective
(easily happened in 40X objective)
Clean it up
There are stains on the lens
(including condenser, objective,
eyepiece and collector)
Clean it up
The oil objective didn
’t immerse oil
Use immerse oil
There was bleb in the oil
Eliminate the bleb
Use a unsuitable oil
Change to the specified one
The size of the aperture diaphragm is
too large
Minify it
The size of the aperture diaphragm is
too small
Open it up
The position of the condenser is too
low
Adjust the position