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Refrigerated Centrifuge Sigma 6-16KS

2 Layout and mode of operation

12 / 91

Version 06/2012, Rev. 1.11 of 06/04/2016 • sb

Translation of the original operating manual, part no. 0702202

Mains cable
(GMP version)

Cooling water
connections
(see chapter 5.3 -
"Special equipment:
water cooling
system")

Fig. 3: Rear view of a water-cooled centrifuge (GMP version)

Pos : 17 / 010 U ni vers al module/ Leerz eile @ 0\ mod_1202116244500_0. doc x @ 114 @ @ 1

Pos : 18 / 100 Sigma/ 110 BA Zentrif ugen Sigma (Pr ojekte) /6- 16KS_6-16KH S/ 020 Aufbau und Wir kungs weise020- 0010-0020 T ypenschil d 6-16KS @ 29\ mod_1405327178393_68.doc x @ 199297 @ 3 @ 1

2.1.2

Name plate

Manufacturer and
registered office

Type

Serial number

Max. speed

Max. kinetic energy

Max. density

Nominal voltage

Input fuse

Symbol for special
disposal (see chapter
9 - "Disposal")

CE mark in compliance
with the directive
2006/42/EC

Part number

Year of manufacture

Power consumption

Fig. 4: Example of a name plate

Pos : 19 / 010 U ni vers al module/ Seitenwec hsel @ 0\ mod_1202116244312_0. doc x @ 105 @ @ 1

Содержание 6-16KS

Страница 1: ...ation of the original operating manual sb 06 2012 Rev 1 11 of 06 04 2016 Sigma 6 16KS part no 0702202 Sigma 6 16KS from serial no 146304 Refrigerated Centrifuge Operating Manual Please retain for late...

Страница 2: ...Refrigerated Centrifuge Sigma 6 16KS 2 91 Version 06 2012 Rev 1 11 of 06 04 2016 sb Translation of the original operating manual part no 0702202...

Страница 3: ...erating manual part no 0702202 In case of inquiries please state the following numbers Order number Serial number Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz...

Страница 4: ...Refrigerated Centrifuge Sigma 6 16KS 4 91 Version 06 2012 Rev 1 11 of 06 04 2016 sb Translation of the original operating manual part no 0702202...

Страница 5: ...force 14 2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Operating personnel 17 3 5 Informal safety inst...

Страница 6: ...ing and closing the lid 28 6 2 2 Installation of rotors and accessories 28 6 2 2 1 Installation of the rotor 28 6 2 2 2 Installation of angle rotors with a hermetically sealed lid 29 6 2 2 3 Installat...

Страница 7: ...trifuge 61 8 1 2 Condenser only refrigerated centrifuges 62 8 1 3 Accessories 62 8 1 3 1 Plastic accessories 63 8 1 4 Rotors buckets and carriers 63 8 1 5 Load bearing bolts 63 8 1 6 Glass breakage 64...

Страница 8: ...Refrigerated Centrifuge Sigma 6 16KS Table of contents 8 91 Version 06 2012 Rev 1 11 of 06 04 2016 sb Translation of the original operating manual part no 0702202...

Страница 9: ...rdmodule 010 Allgemeine Infor mationen010 0020 Besti mmungsgem e Ver wendung 26 mod_1405318951312_68 docx 191709 2 1 1 2 Intended use Centrifuges are power driven machines that separate liquids from s...

Страница 10: ...0 N or men und Vorschriften 26 mod_1405318958202_68 docx 191765 2 1 1 5 Standards and regulations EC declaration of conformity see chapter 11 6 EC declaration of conformity Pos 10 010 U ni versal modu...

Страница 11: ...andar dmodule 020 Aufbau und Wir kungsweise020 0010 Aufbau der Zentrifuge 26 mod_1405318982945_68 docx 191793 2 1 2 1 Layout of the centrifuge Pos 16 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 6 16K...

Страница 12: ...x 114 1 Pos 18 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 6 16KS_6 16KH S 020 Aufbau und Wir kungsweise020 0010 0020 Typenschil d 6 16KS 29 mod_1405327178393_68 docx 199297 3 1 2 1 2 Name plate 1 Ma...

Страница 13: ...function of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor also increa...

Страница 14: ...rce will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitational field diagram provides an ov...

Страница 15: ...ution Risk of bruising Name plate see chapter 2 1 2 Name plate Protective earth ground CE mark in compliance with the directive 2006 42 EC Earth ground Do not dispose as part of domestic waste Unplug...

Страница 16: ...ies DANGER This symbol stands for a direct hazard to the life and health of persons due to electrical voltage Non observance of these symbols causes serious health problems up to life endangering inju...

Страница 17: ...s 36 010 U ni versal module Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 37 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit030 0040 Bedi enpersonal 26 mod_1405319017634_68 docx 19...

Страница 18: ...icher heit030 0060 0020 Mechanische Sicher heit au er 1 16 1 16K 26 mod_1405319024314_68 docx 192059 3 1 3 6 2 Mechanical safety In order to ensure the safe operation of the centrifuge observe the fol...

Страница 19: ...Sigma Standar dmodule 030 Sicher heit030 0060 0040 C hemische und biol ogische Sicherheit 26 mod_1405319028141_68 docx 192101 3 1 3 6 4 Chemical and biological safety If pathogenic toxic or radioacti...

Страница 20: ...ing vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of the rotor Observe the instructions on the installation...

Страница 21: ...ks material abrasion etc After 10 years they must be inspected by the manufacturer After 50 000 cycles the rotor must be scrapped for reasons of safety If other data concerning the service life are en...

Страница 22: ...12 mod_1337063062114_0 docx 65687 1 Pos 63 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit030 0070 0040 Schutzl eiterpr fung Spi ncontr ol L S Easy U ni versal 27 mod_1405319043129_...

Страница 23: ...0 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit030 0100 Restrisi ken 27 mod_1405319052885_68 docx 192339 2 1 3 9 Remaining hazards The unit was built state of the art and according to the accep...

Страница 24: ...161442_68 docx 192367 2 1 4 2 Storage conditions The centrifuge can be stored in its original packaging for up to a year Store the centrifuge only in dry rooms The permissible storage temperature is b...

Страница 25: ...ansport040 0050 Tr ansportsicher ung 6 16KS_KH S berschrift 29 mod_1405327206740_68 docx 199367 2 1 4 5 Transport safety device Pos 86 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 6 16KS_6 16KH S 040...

Страница 26: ...ain Pos 91 010 U ni versal module Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 92 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 050 Aufstellung und Anschl uss050 0020 Energieversorgung 27 mod_1...

Страница 27: ...rant We recommend using a filter to be provided by the customer The operating pressure of the water must be between 1 5 and 5 bar We recommend using a pressure reducer to be provided by the customer T...

Страница 28: ...9 3 1 6 2 1 Opening and closing the lid The lid can be opened if the centrifuge is at a standstill and if the lid key is illuminated Press the lid key in order to open the lid The centrifuge cannot be...

Страница 29: ...eb060 0020 0020 0020 Ei nsetzen von Wi nkelr otoren mit hermetisch verschlie barem D eckel 29 mod_1405327243562_68 docx 199437 4 1 6 2 2 2 Installation of angle rotors with a hermetically sealed lid S...

Страница 30: ...ssories Only use inserts that are suitable for the rotor see chapter 11 1 Range of accessories All buckets of the swing out rotor need to be installed when spinning Always load the axial symmetrical i...

Страница 31: ...13 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 114 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 060 Betrieb_Spi ncontr ol L S060 0020 0020 0040 Adapter nur L S 27 mod_...

Страница 32: ...odul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 118 100 Sig ma 110 BA Zentrifugen Sig ma Proj ekte 6 16KS_6 16KHS 060 Betri eb060 0020 0020 0060 Bl utbeutelsysteme 29 mod_1405327244454_68 docx 1...

Страница 33: ...able curves NOTE If the centrifuge does not reach the mentioned RCF values the maximum gravitational field must be set and the run time must be extended Pos 119 010 Uni versalmodul e Leerzeile 0 mod_1...

Страница 34: ...0193718_68 docx 193123 3 1 6 3 1 User interface The centrifuge is operated via three buttons with integrated light emitting diodes and one function knob The display is divided into several different f...

Страница 35: ...st be opened before a new centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is triggered Qui...

Страница 36: ...are stated in revolutions per minute min 1 rpm and depend on the RCF values see chapter 2 2 2 1 Speed radius and relative centrifugal force The maximum speed values depend on the rotor that is used Re...

Страница 37: ...ached Select the field Runtime and confirm the selection The display flashes when it is activated Turn the function knob from the time 0 00 10 anti clockwise or from the time 99 59 59 clockwise Infini...

Страница 38: ...e data Fig 15 Rotor selection list here with potential rotor bucket combinations and additional information Automatic rotor identification The centrifuge automatically identifies the rotor that is cur...

Страница 39: ...enu shows the program that is currently loaded When the field is selected the program list is displayed for information on how to work with the programs please see chapter 6 3 3 Program mode The progr...

Страница 40: ...is case the RAPID_TEMP program stops with a sound signal and the standstill cooling system will be activated The stop button is pressed The RAPID_TEMP program will be stopped prematurely No message wi...

Страница 41: ...he actual sample temperature Progress indicator The progress indicator provides a quick overview of the remaining runtime of the running centrifugation run For this purpose a green progress bar and pe...

Страница 42: ...erature exception the RAPID_TEMP program will not be listed The order of the processes corresponds to their storage locations on the program list Empty storage locations will not be displayed If more...

Страница 43: ...ooled samples in the uncooled centrifuge from heating up to an inadmissible temperature level If the standstill cooling function is activated the centrifuge starts to precool after it is switched on I...

Страница 44: ...oring function enables the continuous monitoring of the speed and runtime parameters during the centrifugation Activate the centrifugation monitoring function by clicking NOTE If the function is activ...

Страница 45: ...tarted if the current temperature is between the preset temperature in the Standard menu and the Delta T limit value If the temperature leaves this range during the centrifugation run an error message...

Страница 46: ...ction will also be selected Select the button Activate code Enter a four digit code and confirm the entry The lock is now active The symbol will be displayed in the status line and the lock level will...

Страница 47: ...les the optimum adaptation of the centrifuge to its specific area of application Fig 23 Menu Setup Function Open lid after run The automatic lid opening function ensures that the lid opens when the ro...

Страница 48: ...be displayed in C Celsius or F Fahrenheit Language The control system can be used in various language versions If a language is selected by mistake it can be changed on any screen as follows Press an...

Страница 49: ...hen the cursor flashes in the text field Turn the function knob in order to select a character and press it to confirm the selection Then press the knob again in order to enter the next character Pres...

Страница 50: ...e editor will be displayed Select a curve number between 20 and 29 in the input field Curve If the curve number is already used the stored curve will be displayed The input field Int is used to specif...

Страница 51: ...necessary slope 0 03 min 1 rpm which means that it is beyond the defined range Example 2 Start speed 0 min 1 rpm final speed 15000 min 1 rpm runtime 10 sec not possible since the necessary slope is 15...

Страница 52: ...ifugen Sig ma Standardmodul e 060 Betrieb_Spi ncontr ol L S Spincontrol S Zent7 060 0030 0030 Pr ogrammbetrieb 27 mod_1405320235093_68 docx 193459 3 1 6 3 3 Program mode A program contains all of the...

Страница 53: ...he selection Then the next character can be selected Pressing the arrow button will delete the last character When the program name is complete select OK and confirm it The program will be saved and t...

Страница 54: ...onfirm the selection by pressing the function knob Press the start button Or Open the process library select the desired program and press the start button The program will be executed and the Standar...

Страница 55: ...tup menu Fig 30 Program rotation function When a program is loaded while the program rotation function is active this program will be used as the start program for the rotation After the completion of...

Страница 56: ...centrifuge type partly standard External signal active DC 24V 0 5 A max part no 17701 Floating switch AC 250V max 6 A part no 17702 Pos 161 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx...

Страница 57: ...ction Mains power switch off Switch mains power switch on Centrifuge cannot be started start key LED is not illuminated Several Power off on If the error occurs again contact service Centrifuge cannot...

Страница 58: ...e right side of the control panel e g with a screwdriver Fig 32 Position of the opening for the emergency lid release Insert the supplied hexagon socket key horizontally into the hole The key will be...

Страница 59: ...erature error only for refrigerated centrifuges Allow to slow down Power off Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water co...

Страница 60: ...es malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma zentrifugen d...

Страница 61: ...thermal stress e g by heat generators Pos 176 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 177 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 080 War tung und Instandhal...

Страница 62: ...Zubeh r au er 8K 28 mod_1405320618247_68 docx 194845 3 1 8 1 3 Accessories CAUTION For the care of the accessories special safety measures must be considered as these are measures that will ensure ope...

Страница 63: ...Chemical reactions as well as stress corrosion combination of oscillating pressure and chemical reaction can affect or destroy the metals Barely detectable cracks on the surface can expand and weaken...

Страница 64: ...pper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2000 rpm The glass and metal particles will now collect at the greased part Rem...

Страница 65: ...s Max temp C Min time min Max time min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 30 40 Polya...

Страница 66: ...f the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especially the rubber parts that are among other things part of the motor suspension are subject to age...

Страница 67: ...e are obliged to certify the harmlessness of all incoming goods For this purpose we require a declaration of decontamination The form must be filled in completely and signed by authorised and speciali...

Страница 68: ...lease use the original packaging if at all possible If the product is dispatched to us in unsuitable packaging you will be charged the cost for returning it to you in new packaging The forms can be do...

Страница 69: ...sible to dispose of the unit among household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of decontam...

Страница 70: ...kVA Power consumption kW Max current consumption A Input fuse AT 2 8 2 3 14 0 at 220 240 V 50 Hz 16 0 at 220 240 V 50 Hz temperature fuses Performance data Max speed rpm Max capacity ml Max gravitatio...

Страница 71: ...o 35 C Max relative humidity of air 80 up to 31 C with a linear decrease to 67 relative humidity of air at 35 C Maximum altitude 2 000 m above sea level Pos 211 010 Uni versalmodul e Leerzeile 0 mod_1...

Страница 72: ...dius min 8 8 cm 5 100 5 292 11152 Swing out rotor 96 x 15 ml complete no 11150 incl 1 set double rectangular buckets no 13420 4 sets rectangular buckets no 18015 and 96 PPCO tubes 15 ml no 15023 Radiu...

Страница 73: ...afe with certificate culture tubes e g no 15151 incl bayonet lid no 17604 Radius max 10 4 cm radius min 4 3 cm angle 25 13 500 21 191 12356 Angle rotor 6 x 250 ml Biosafe with certificate for bottles...

Страница 74: ...8250 18615 18650 max tube length 110 mm 1 set 4 pcs Radius max 19 3 cm radius min 10 5 cm Attention The buckets are dynamically balanced must be installed in sets of 4 and in the prescribed order see...

Страница 75: ...uitable for 12130 12449 13055 Stainless steel tube 50 ml 28 5 x 101 5 mm sealable with cap no 17054 suitable for 12266 13082 13062 Adapter for 1 Greiner tube 15 ml 17 22 x 95 100 mm suitable for 12266...

Страница 76: ...the 500 ml bottles use the supplied supporting rings When using glass tubes the maximum value of 4 000 x g must not be exceeded except special high strengh glass tubes please refer to the information...

Страница 77: ...g manual part no 0702202 Pos 222 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 110 Anhang110 0020 Drehzahl Schwerefel d Diagramm 28 mod_1405320705642_68 docx 195153 2 1 11 2 Speed gravitational...

Страница 78: ...e fixed acceleration curves defines the time that is required to accelerate the rotor by 1 000 rpm Curve 9 is a special case compared to the other curves The centrifuge accelerates with maximum power...

Страница 79: ...300 400 500 Time sec Speed rpm curve 10 curve 11 curve 12 curve 13 curve 14 curve 15 curve 16 curve 17 curve 18 curve 19 18 19 17 16 15 14 13 12 11 10 Fig 40 Diagram of quadratic curves Pos 225 010 U...

Страница 80: ...years 20x 1 14 1 14K 12096 5 years 20x 1 14 1 14K 12101 5 years 20x 1 15 1 15K 1 15P 1 15PK 12124 5 years 20x 1 15 1 15K 1 15P 1 15PK 12126 5 years 20x 1 15 1 15K 1 15P 1 15PK 12134 5 years 20x 1 16 1...

Страница 81: ...Acetone C3H6O 100 1 1 4 1 1 4 4 1 4 1 Acrylonitrile C3H3N 100 1 1 4 3 3 4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Aluminium sulfate...

Страница 82: ...ne C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylaniline C8H11N...

Страница 83: ...3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1 1 1 1 1 Nit...

Страница 84: ...1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1 4 1 3 4 4 1 4 1 Sulphuric acid H2SO4...

Страница 85: ...6 04 2016 sb 85 91 Translation of the original operating manual part no 0702202 Pos 230 100 Sig ma 110 BA Zentrifugen Sig ma Proj ekte 6 16KS_6 16KHS 110 Anhang110 0060 Konfor mit tser kl rung 6 16KS_...

Страница 86: ...Refrigerated Centrifuge Sigma 6 16KS 11 Appendix 86 91 Version 06 2012 Rev 1 11 of 06 04 2016 sb Translation of the original operating manual part no 0702202 Ende der Liste f r Textmar ke Inhalt...

Страница 87: ...with the directive 2006 42 EC 15 Centrifugation monitoring 44 Centrifugation of blood bag systems 32 Centrifugation of infectious toxic radioactive or pathogenic substances 19 Centrifugation principl...

Страница 88: ...58 Equipotential bonding screw 22 Error correction 57 Error message 57 Executing a program 54 Explanation of the symbols and notes 16 Explosive substances 19 External signal 49 F Fire preventions 19...

Страница 89: ...odification mode active 35 Motor error 59 Motor shaft 28 29 61 Multiple carrier 32 N Name plate 11 12 No indication on the display 57 No power in the mains supply 57 Noise level 70 Non greased bolts 6...

Страница 90: ...fety instructions for centrifugation 20 Safety chemical and biological 19 Safety electrical 18 Safety mechanical 18 Safety conscious work 17 Saving a program 53 Scope of supply 10 Screws of the transp...

Страница 91: ...monitoring 45 Temperature monitoring system 22 Temperature unit 48 Temperature value not reached 57 Thermal stress 61 Time fine 48 Time range 70 Toxic substances 19 61 Transport safety device 25 Tubes...

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