Operating Manual SIGMA 2-5, page 39 of 55
03/06
5.1
Practical Notes for Centrifugation
1. Locate centrifuge horizontally on a level surface.
2. Ensure safe location.
3. Keep at least 30 cm free space around the centrifuge.
4. Provide for sufficient ventilation.
5. Tighten rotor firmly onto motor shaft.
6. Avoid imbalance.
7. Load opposite places with same accessories.
8. Centrifugation with low capacity:
An example is the fixed angle rotor 24 x
2,2 ml and the rotor 8 x 15 ml which
should be loaded with 4 vessels only.
The vessels should be placed symmetri-
cally so that the rotor is loaded evenly.
Loading e.g. only one position is not
allowed.
9. Load all positions of swing-out rotors.
10. Load vessels outside the centrifuge.
11. Fill vessels carefully to same weight.
Imbalances would result in increased
wear of bearings.
12. Use perfect accessories only.
13. Avoid corrosion to accessories by careful maintenance.
14. Spin infectious material in sealed rotors and buckets only.
15. Do not spin explosive or highly imflammable materials.
16. When centrifuging substances with a density > 1,2 g/cm
3
the allowable max.
speed must be reduced (refer to chapter 7.1.2 "Density").
17. Grease the bolts of the rotor and the nutes of the buckets slightely with
grease for load bearing bolds.
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