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Refrigerated Centrifuge Sigma 1-14K 

  

11 Appendix 

 

 

 

Version 05/2010, Rev. 2.12 of 15/08/2016 • sb 

61 / 70 

Translation of the original operating manual, part no. 0700102 

 

-  no data 
1 resistant 
2 practically resistant 
3 partially resistant 
4 not resistant 
 

  

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A

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Medium 

Formula 

 [

%

] 

H

D

P

E

 

P

A

 

 P

C

 

P

O

M

 

P

P

 

P

S

U

 

P

V

C

 

P

V

C

 

P

T

F

E

 

N

B

R

 

A

L

 

Isopropyl alcohol 

C

3

H

8

O 

100

1 

1 

1 

1 

1 

1 

1 

4 

1 

- 

2 

Lactic acid 

C

3

H

6

O

3

 

3

1 

3 

1 

2 

1 

1 

2 

- 

1 

1 

1 

Magnesium chloride 

MgCl

2

 

10

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

Mercuric chloride 

HgCl

2

 

10

1 

4 

1 

3 

1 

1 

1 

1 

1 

1 

4 

Mercury 

Hg 

100

1 

1 

1 

1 

1 

1 

1 

3 

1 

1 

3 

Methyl acetate 

C

3

H

6

O

2

 

100

1 

1 

4 

2 

1 

- 

4 

4 

1 

- 

1 

Methyl alcohol 

CH

4

O 

100

1 

2 

4 

1 

1 

3 

1 

3 

1 

2 

1 

Methyl benzene 

C

7

H

8

 

100

3 

1 

4 

1 

3 

4 

4 

4 

1 

4 

1 

Methyl ethyl ketone 
(MEK) 

C

4

H

8

O 

100

1 

1 

4 

1 

1 

4 

4 

4 

1 

4 

1 

Methylene chloride 

CH

2

Cl

2

 

100

4 

3 

4 

3 

3 

4 

4 

4 

1 

- 

1 

Mineral oil 

— 

100

1 

1 

1 

1 

1 

1 

1 

- 

1 

1 

1 

Nitric acid 

HNO

3

 

10

1 

4 

1 

4 

1 

1 

1 

- 

1 

4 

3 

Nitric acid 

HNO

3

 

100

4 

4 

4 

4 

4 

- 

4 

- 

1 

4 

1 

Nitrobenzene 

C

6

H

5

NO

2

 

100

3 

4 

4 

3 

2 

4 

4 

4 

1 

4 

1 

Oleic acid 

C

18

H

34

O

2

 

100

1 

1 

1 

2 

1 

- 

1 

- 

1 

3 

1 

Oxalic acid 

C

2

H

2

O

4

 x 2H

2

O 

100

1 

3 

1 

4 

1 

1 

1 

1 

1 

2 

1 

Ozone 

O

3

 

100

3 

4 

1 

4 

3 

1 

1 

- 

1 

4 

2 

Petroleum 

— 

100

1 

1 

3 

1 

1 

1 

1 

3 

1 

1 

1 

Phenol 

C

6

H

6

O 

10

1 

4 

4 

4 

1 

4 

1 

3 

1 

3 

1 

Phenol 

C

6

H

6

O 

100

2 

4 

4 

4 

1 

3 

4 

3 

1 

3 

1 

Phosphoric acid 

H

3

PO

4

 

20

1 

4 

2 

4 

1 

- 

- 

- 

1 

2 

4 

Phosphorus 
pentachloride 

PCl

5

 

100

- 

4 

4 

4 

1 

- 

4 

4 

1 

- 

1 

Potassium hydrogen 
carbonate 

CHKO

3

 

saturated 1 

1 

2 

1 

1 

- 

- 

- 

1 

- 

4 

Potassium hydroxide 

KOH 

30

1 

1 

4 

3 

1 

1 

1 

1 

1 

- 

4 

Potassium hydroxide 

KOH 

50

1 

1 

4 

3 

1 

1 

1 

1 

1 

- 

4 

Potassium nitrate 

KNO

3

 

10

1 

1 

1 

1 

1 

- 

- 

- 

1 

1 

1 

Potassium 
permanganate 

KMnO

4

 

100

1 

4 

1 

1 

1 

- 

1 

- 

1 

3 

1 

Pyridine 

C

5

H

5

N 

100

1 

1 

4 

1 

3 

4 

4 

4 

1 

4 

1 

Resorcinol 

C

6

H

6

O

2

 

5

1 

4 

2 

3 

1 

4 

2 

- 

1 

- 

2 

Silver nitrate 

AgNO

3

 

100

1 

1 

1 

1 

1 

1 

1 

1 

1 

2 

4 

Содержание 1-14K

Страница 1: ...lation of the original operating manual sb 05 2010 Rev 2 12 of 15 08 2016 Sigma 1 14K part no 0700102 Sigma 1 14K from serial no 135550 Refrigerated Centrifuge Operating Manual Please retain for later...

Страница 2: ...Refrigerated Centrifuge Sigma 1 14K 2 70 Version 05 2010 Rev 2 12 of 15 08 2016 sb Translation of the original operating manual part no 0700102...

Страница 3: ...erating manual part no 0700102 In case of inquiries please state the following numbers Order number Serial number Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz...

Страница 4: ...Refrigerated Centrifuge Sigma 1 14K 4 70 Version 05 2010 Rev 2 12 of 15 08 2016 sb Translation of the original operating manual part no 0700102...

Страница 5: ...l force 14 2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Operating personnel 17 3 5 Informal safety in...

Страница 6: ...31 6 3 Control System Spincontrol Basic 32 6 3 1 User interface 32 6 3 2 Display 32 6 3 3 Starting a centrifugation run 33 6 3 4 Interrupting a centrifugation run 33 6 3 5 Interrupting a deceleration...

Страница 7: ...e 46 8 2 Sterilisation and disinfection of the rotor chamber and accessories 46 8 2 1 Autoclaving 47 8 3 Service 48 8 4 Return of defective parts 49 9 Disposal 51 9 1 Disposal of the centrifuge 51 9 2...

Страница 8: ...Refrigerated Centrifuge Sigma 1 14K Table of contents 8 70 Version 05 2010 Rev 2 12 of 15 08 2016 sb Translation of the original operating manual part no 0700102...

Страница 9: ...r dmo dule 01 0 Allge meine Inf orm ation en 010 00 20 Bes tim mung sge m e Verw end ung 26 mod _14 053 189 513 12_ 68 d ocx 191 709 2 1 1 2 Intended use Centrifuges are power driven machines that sep...

Страница 10: ...mein e In for matio nen 010 0 050 Nor men un d Vorsc hrift en 26 mod _14 053 1895 820 2_6 8 do cx 1 917 65 2 1 1 5 Standards and regulations EC declaration of conformity see chapter 11 5 EC declaratio...

Страница 11: ...uge 26 mo d_1 405 318 982 945_ 68 docx 19 179 3 2 1 2 1 Layout of the centrifuge Pos 17 010 Unive rsalm odul e L ee rzeile 0 mod _120 211 624 450 0_0 docx 11 4 1 Pos 18 100 Sigma 110 BA Ze ntrifu gen...

Страница 12: ...schild 1 14K 28 m od_ 140 532 076 137 2_68 docx 1 953 07 3 1 2 1 2 Name plate 1 Manufacturer and registered office 2 Type 3 Serial number 4 Max speed 5 Max kinetic energy 6 Max density 7 Nominal volta...

Страница 13: ...function of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor also increa...

Страница 14: ...recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitational field diagram provides an overview of th...

Страница 15: ...540 400 343 1_6 8 doc x 2 039 35 2 1 3 1 Marking of the unit The following symbols are used for all types of centrifuges manufactured by Sigma Dangerous voltage I On Power Hot surface 0 Off Power Cau...

Страница 16: ...his operating manual the following names and symbols to indicate hazards are used DANGER This symbol stands for a direct hazard to the life and health of persons Non observance of these symbols causes...

Страница 17: ...odul e L ee rzeile 0 mod _120 211 624 450 0_0 docx 11 4 1 Pos 40 100 Sigma 100 BA Ze ntrifu gen Sigma Stan da rdm odule 0 30 Sich erh eit 03 0 0 040 Bedi enp erso nal 26 mod _14 053 1901 763 4_6 8 do...

Страница 18: ...rdm odule 0 30 Sich erh eit 03 0 0 060 00 20 Mec hanisc he Sich erh eit au er 1 1 6 1 16K 2 6 m od_1 405 319 024 314 _68 docx 19 205 9 3 1 3 6 2 Mechanical safety In order to ensure the safe operation...

Страница 19: ...fu gen Sigma Stan da rdm odule 0 30 Sich erh eit 03 0 0 060 00 40 Che mische un d biolo gisch e Sicher heit 26 mo d_1 405 3190 281 41_ 68 d ocx 192 101 3 1 3 6 4 Chemical and biological safety If path...

Страница 20: ...els can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of the rotor Observe the instructions on the installation of acces...

Страница 21: ...t least once per month for safety reasons Pay special attention to changes such as corrosion cracks material abrasion etc After 10 years they must be inspected by the manufacturer After 50 000 cycles...

Страница 22: ...ions are indicated by error messages with a number in the speed rcf display see chapter 7 2 Table of error codes Pos 64 010 Unive rsalm odul e L ee rzeile 0 mod _120 211 624 450 0_0 docx 11 4 1 Pos 65...

Страница 23: ...e run g u nd Tra nspo rt 0 40 002 0 L age rbe ding ung en 27 mod _14 0531 916 144 2_6 8 do cx 1 923 67 2 1 4 2 Storage conditions The centrifuge can be stored in its original packaging for up to a yea...

Страница 24: ...When lifting the centrifuge always reach under the centrifuge from the side CAUTION The centrifuge weighs approx 19 kg Retain the packaging for any possible future transport of the centrifuge Pos 80...

Страница 25: ...icient time for drying min 24 h before the centrifuge can be used again Pos 86 010 Unive rsalm odul e L ee rzeile 0 mod _120 211 624 450 0_0 docx 11 4 1 Pos 87 100 Sigma 100 BA Ze ntrifu gen Sigma Sta...

Страница 26: ...ee rzeile 0 mod _120 211 624 450 0_0 docx 11 4 1 Pos 98 100 Sigma 100 BA Ze ntrifu gen Sigma Stan da rdm odule 0 61 Bet rieb _Spinco ntr ol Basic 0 61 002 0 0 010 ffn en u nd Schli e e n d es Deck el...

Страница 27: ...onical spring washer Fig 6 Rotor fastening nut from above A and from below B To lock the snap on lid pull the safety lock in the middle of the lock upwards and put the lid on the rotor The snap on lid...

Страница 28: ...anently joined to the rotor cross Do not disassemble it Do not use filters in the vessels Load the rotor prior to installing it in the centrifuge In the case of big vessels e g 15040 insert the vessel...

Страница 29: ...Bet rieb 060 00 20 002 0 0 020 Einsetz en d es Mik roh ma tokrit telle rs 1 14_ 1 1 4K 2 8 m od_ 140 532 0812 176 _68 docx 1 9548 9 4 1 6 2 2 3 Installation of the microhaematocrit rotor Fig 9 Microh...

Страница 30: ...id Enter the following parameters speed 13 000 rpm gravitational field RCF max 11 903 x g time approx 5 min Start the centrifuge Open the centrifuge lid when the rotor has stopped Unlock the rotor cov...

Страница 31: ...etrically so that the rotor is loaded evenly It is not permissible to load angle rotors on only one axis correct incorrect Fig 11 Permissible and impermissible loading of an angle rotor example illust...

Страница 32: ...ram key 4 Arrow keys 5 Lid key 6 Start Stop key 7 Precool key Fig 12 User interface The centrifuge is started directly via the user interface When the centrifuge is switched on all segments will be il...

Страница 33: ...1 01 0 Univ ersal mod ule Lee rzeile 0 mo d_12 021 162 445 00_ 0 doc x 1 14 1 Pos 122 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd mod ule 061 B etrie b_Spinc ont rol Basic Spi ncon trol Ba sic...

Страница 34: ...w hr end des Lau fs 2 7 m od_ 140 532 035 828 2_68 docx 1 938 79 4 1 6 3 7 1 Changing the runtime during centrifugation The preselected runtime can be changed during centrifugation Press the set key r...

Страница 35: ...front of the temperature value Temperatures between 10 C and 40 C can be selected Press the set key until the temperature unit flashes on the display Select the desired temperature by pressing the arr...

Страница 36: ...c Spi ncon trol Ba sic 06 1 0 030 00 90 So ftsta rt und S oftsto p Funk tione n 27 mod_ 140 532 036 594 4_6 8 docx 1 939 49 3 1 6 3 9 Softstart and softstop function The softstart function is used to...

Страница 37: ...ther rotor is installed the configuration must be changed so that the allowed maximum speed of the rotor can be reached To select another rotor press the set key and hold it for approx 2 seconds Choos...

Страница 38: ...er to save the data start the centrifuge or press the set key repeatedly until the indication Prog stops flashing NOTE Program numbers that are already occupied will be overwritten with the current da...

Страница 39: ...played This is necessary in order to ensure that the rotor is at a complete standstill Eliminate the source of the problem see tables below Acknowledge the error messages with the lid key Type of erro...

Страница 40: ...oden logs Ensure that the centre of gravity of the centrifuge is still located above the table top If necessary the unit must be secured by a second person Insert the spanner for the emergency release...

Страница 41: ...45 Temperature error only for refrigerated centrifuges Allow to slow down Power off Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only w...

Страница 42: ...ies malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma zentrifugen...

Страница 43: ...ve UV radiation or thermal stress e g by heat generators Pos 163 01 0 Univ ersal mod ule Lee rzeile 0 mo d_12 021 162 445 00_ 0 doc x 1 14 1 Pos 164 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd...

Страница 44: ...ule 080 W art ung und Inst andh altun g 0 80 001 0 0 020 Zube h r au e r 8K 28 mo d_1 405 320 618 247_ 68 docx 19 484 5 3 1 8 1 3 Accessories CAUTION For the care of the accessories special safety mea...

Страница 45: ...recision in order to withstand the permanent high stress from high gravitational fields Chemical reactions as well as stress corrosion combination of oscillating pressure and chemical reaction can aff...

Страница 46: ...the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2000 rpm The glass and metal particles will now collect at the greased part Remove the greas...

Страница 47: ...sories Max temp C Min time min Max time min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 30 40...

Страница 48: ...ction of the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especially the rubber parts that are among other things part of the motor suspension are subject...

Страница 49: ...we are obliged to certify the harmlessness of all incoming goods For this purpose we require a declaration of decontamination The form must be filled in completely and signed by authorised and special...

Страница 50: ...he original packaging for the unit if at all possible If the product is dispatched to us in unsuitable packaging you will be charged the cost for returning it to you in new packaging The forms can be...

Страница 51: ...ld waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of decontamination see chapter 8 4 Return of defectiv...

Страница 52: ...99 min 59 sec short run continuous run 10 C to 40 C Physical data Height mm Height with opened lid mm Width mm Depth mm Weight kg 236 404 273 515 19 0 Noise level dB A 59 at max speed Pos 198 01 0 Uni...

Страница 53: ...6 9_6 8 doc x 1 950 55 2 1 10 2 Technical documentation For environmental reasons the comprehensive technical documentation of the centrifuge e g circuit diagrams and the safety data sheets of the man...

Страница 54: ...vials e g no 15008 15040 incl polysulfone lid no 17882 Radius max 6 6 cm radius min 3 8 and 3 0 cm angle 32 37 15 000 16 602 12092 Angle rotor made of polypropylene 12 x 1 5 2 0 ml for reaction vials...

Страница 55: ...mod _14 053 207 036 31_6 8 d ocx 195 125 3 1 11 1 1 Rotor radii The information in the accessories table concerning the radius refers to the values of the respective rotor as shown below The radius c...

Страница 56: ...00102 Pos 210 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd mod ule 110 A nha ng 1 10 002 0 Dr ehza hl Schw eref eld Di agr am m 28 m od_ 140 532 070 564 2_68 docx 1 951 53 2 1 11 2 Speed gravit...

Страница 57: ...096 5 years 20x 1 14 1 14K 12097 5 years 20x 1 14 1 14K 12101 5 years 20x 1 15 1 15K 1 15P 1 15PK 12124 5 years 20x 1 15 1 15K 1 15P 1 15PK 12126 5 years 20x 1 15 1 15K 1 15P 1 15PK 12134 5 years 20x...

Страница 58: ...05 2010 Rev 2 12 of 15 08 2016 sb Translation of the original operating manual part no 0700102 Pos 214 10 0 Sigm a 10 0 BA Zentrif uge n Sigm a Sta nda rd mod ule 110 A nha ng 1 10 005 0 Best ndi gke...

Страница 59: ...4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Aluminium sulfate Al2 SO4 3 10 1 1 1 3 1 1 1 1 1 1 1 Ammonium chloride NH4 Cl aqueous 1 1...

Страница 60: ...e C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylaniline C8H11N 1...

Страница 61: ...3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1 1 1 1 1 Nit...

Страница 62: ...1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1 4 1 3 4 4 1 4 1 Sulphuric acid H2SO4 6 1 4...

Страница 63: ...016 sb 63 70 Translation of the original operating manual part no 0700102 Pos 216 10 0 Sigm a 11 0 BA Zentrif uge n Sigm a Pr ojekt e 1 14 _1 14K 1 10 An hang 11 0 0 060 Konf ormi t tse rkl rung 1 14K...

Страница 64: ...Refrigerated Centrifuge Sigma 1 14K 11 Appendix 64 70 Version 05 2010 Rev 2 12 of 15 08 2016 sb Translation of the original operating manual part no 0700102...

Страница 65: ...Refrigerated Centrifuge Sigma 1 14K 11 Appendix Version 05 2010 Rev 2 12 of 15 08 2016 sb 65 70 Translation of the original operating manual part no 0700102 11 6 Declaration of conformity China RoHS 2...

Страница 66: ...Refrigerated Centrifuge Sigma 1 14K 11 Appendix 66 70 Version 05 2010 Rev 2 12 of 15 08 2016 sb Translation of the original operating manual part no 0700102 E nde der Liste f r Tex tma rke I nhal t...

Страница 67: ...Chemical resistance of plastic 45 China RoHS 2 Declaration of conformity 65 Cleaning agents 46 Cleaning the bores of angle rotors 44 Cleaning the centrifuge 43 Closing the lid 18 26 Communication err...

Страница 68: ...of rotors and accessories 26 Installation of the microhaematocrit rotor 29 Installation of the rotor 26 Installation of the rotor no 11128 28 Installation site 25 Intended use 9 Interrupting a centrif...

Страница 69: ...life 21 57 Rotors cleaning and care 45 Runtime 34 S Safety area 20 Safety devices 22 Safety distance 18 20 25 Safety instructions 9 27 29 31 Safety instructions for centrifugation 20 Safety of rotors...

Страница 70: ...range 52 Temperature value cannot be reached 39 Thermal stress 43 Time range 52 Toxic substances 19 43 Transport safety device 24 Type 52 Type of connection 25 Type of the centrifuge 42 48 U Units of...

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