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Model 420 & 425 Flame Photometer

425 91 001

Issue 22

December 2016

ECN 1410 & 1932

- 56 -

Accessories Components and Spare Parts

continued

Accessories

420 66 000

BlueNotes 420 Software

473 86 009

Printer assembly, universal

475 41 100

M805 Continuous Flow Diluter for Clinical Samples 1:200
Ratio

860 00 009

Model 860 Autosampler 40 position

851 01 001

Model 851 Air Compressor

855 01 001

Model 855Air Compressor, complete with water separator

9.3

Reagents

Catalogue
#

Description

Quantity

001 56 100 140.0mmol/Na, 5.00mmol K, 1.50mmol/L Li, 100ml

1 Bottle

NOTE:

The following solutions are also available

001 56 620 Flame Photometer Standard, 1000ppm Na, 6 x 100ml

1 Pack

001 56 621 Flame Photometer Standard, 1000ppm K, 6 x 100ml

1 Pack

001 56 622 Flame Photometer Standard, 1000ppm Li, 6 x 100ml

1 Pack

001 56 623 Flame Photometer Standard, 1000ppm Ca, 6 x 100ml

1 Pack

001 56 603 Flame Photometer Standard, 3Molar Li, 100ml

1 Bottle

001 56 681 Diluent Concentrate, 6 x 100ml

1 Pack

001 56 682 Tubing Disinfectant, 6 x 100ml

1 Pack

001 56 183 Deproteinising Solution, 6 x 80ml

1 Pack

Содержание 420

Страница 1: ...NTROL Manuals Flame 425 42591001 M420 M425 Operator Manual Issue 22 doc Model 420 and Model 425 Flame Photometer Operator Manual Sherwood Scientific Limited 1 The Paddocks Cherry Hinton Road Cambridge CB1 8DH United Kingdom Copyright Sherwood Scientific Limited 2017 ...

Страница 2: ... 4 attempts if the instrument has not been used for some time Blanking Zeroing 7 With the 000 flashing aspirate the blank solution and press the Blank button once 8 The instrument will then zero to the blank values this will take at least 30 seconds Remove the blank when complete Calibration 9 When the blanking procedure is complete the previous calibration values that were used will appear in the...

Страница 3: ... 18 Air 18 Waste Container 18 2 3 Unpacking 19 Accessory List 19 2 4 Assembly 20 2 5 Connecting Peripheral Devices 24 Printer 24 Computer 24 Analogue Devices 24 Auto sampler 24 2 6 Setting Date and Time 25 2 7 Setting Filter Factors 26 2 8 Setting Units 26 2 9 Setting Sample Delay in Peak mode 27 2 10 Setting the Output Voltage 27 2 11 Setting the analogue damping coefficient 27 3 PRINCIPLES OF OP...

Страница 4: ...Controls and Indicators 35 5 1 1 Controls 35 Set 35 Blank 36 Single Dual and Element Selection 36 Peak Ref Cont 36 Measure 37 5 1 2 Description of Indicators 37 Cal Ch1 Ch2 37 Single 37 Dual 37 Peak 37 Ref 37 Cont 38 Measure 38 Flame On 38 Power On 38 5 2 Rear Panel Controls and Connections 38 Channel 1 2 Data Outputs 39 RS232 Printer Output 39 External Device Output 39 Power 39 Gas 39 Air 39 Air ...

Страница 5: ...CAUTIONS LIMITATIONS 47 7 1 Hints 47 7 2 Operational Precautions and Limitations 47 7 3 Hazards 48 7 4 Measuring Calcium 48 8 CLEANING AND MAINTENANCE PROCEDURES 49 8 1 General 49 8 2 Daily Maintenance 49 8 3 Weekly Maintenance 49 8 4 Monthly Maintenance 49 8 5 Six Monthly Maintenance 50 8 6 Nebuliser 50 8 6 1 Operational Check 50 8 6 2 Cleaning the Nebuliser 51 8 7 Cleaning the Mixing Chamber Bur...

Страница 6: ...ng using maintaining or repairing the instrument and or its accessories 1 2 Using this manual This manual is structured to allow both quick access to a measurement from the default position of the Instrument Section entitled Short Form Instructions on page 2 and an in depth understanding of the Set up Operation and Maintenance of these two advanced Flame Photometers The information contained in th...

Страница 7: ...ty of your electrical supply system consult a competent qualified electrician There are no user replaceable parts within the instrument Do not remove the rear cover from the instrument Sherwood Scientific Limited and its authorised Distributors consider themselves responsible for the effects of safety reliability and performance of the Model 420 Model 425 only if Assembly operations extensions re ...

Страница 8: ...rect sunlight in a cool place below 25 C 77 F in an airtight container to prevent evapor ation and discolouration Glass containers should not be used as they can affect Na concentration levels Prolonged exposure to the atmosphere must be avoided to prevent evaporation of standard solutions Purification No purification is required for Sherwood Scientific standard solutions 1 5 Dilutions The Model 4...

Страница 9: ...ppm or 0 0005 to 0 5mmol l or 0 0005 to 0 5mEq l 0 02 to 2ppm or 0 0005 to 0 05mmol l or 0 0005 to 0 05mEq l concentrations below 10mmol l or 10mEq diluted 200 times 0 02 to 20ppm or 0 0005 to 0 5mmol l or 0 0005 to 0 5mEq l 1 00 to 10 00 10 0 to 199 0 1 00 to 10 00 10 00 11 0 to 199 0 Lithium Range that standard may be set to 0 05 to 20ppm or 0 0007 to 2 9mmol l or 0 0007 to 2 9mEq l 0 05 to 0 7p...

Страница 10: ... Na K both Serum and Urine 1 200 with diluent described above including Li if used in Reference Mode Li Measurement Dilution Ratio 1 50 In Non Clinical Mode The user should ascertain from his sample what is appropriate by reference to the input concentrations listed in the specification in Section 4 Urine Samples The Potassium content of urine samples is some 20 times that of plasma or serum In th...

Страница 11: ... part diluent concentrate to 999 parts 100ppm Li solution 160mmol l Na 80mmol l K standard diluted one part in five then 200 times with the blank solution Dilute sample one part in five then 200 times with the blank solution Clinical Mode 102 1 part diluent concentrate to 999 parts good quality deionised water The standard contained 160mmol l Na 80mmol l K prior to dilution therefore set the calib...

Страница 12: ...erwood Scientific produce a Dilutor the Model 805 which automates the process and gives extremely reproducible dilution ratios and therefore precise results Remember when using the Model 805 Dilutor to increase the Delay Time see Section 2 9 1 6 BlueNotes A BlueNotes CDROM is provided with this instrument for loading on a PC running Windows and will extend the instruments capability Generate repor...

Страница 13: ...Notes Software installation The BlueNotes 420 Software is supplied on a CD ROM with a unique 16 character alphanumeric registration code Insert the CD ROM into the disk drive of a PC The software should auto run and the following window appear Click on Next to load the software Read the license agreement and click Next ...

Страница 14: ...ssue 22 1st December 2016 ECN 1410 1932 14 Introduction continued 1 6 1 BlueNotes Software Installation continued Record the file path that BlueNotes 420 is to be installed to for future reference click Next Click Next to begin the installation ...

Страница 15: ...n continued 1 6 1 BlueNotes Software Installation continued Click Close to complete the installation process The Desktop will now show the shortcut to BlueNotes 420 Double click the shortcut to start BlueNotes 420 The first time that BlueNotes is started the License number must be entered see below ...

Страница 16: ...otometer 425 91 001 Issue 22 1st December 2016 ECN 1410 1932 16 Introduction 1 6 1 BlueNotes Software Installation continued continued Enter the License Install Key 16 alpha numeric character code supplied with the software ...

Страница 17: ... continued Click Help then User Guide to read or print the full BlueNotes Manual for further comprehensive instructions For an easy to follow guide select Help then Presentations from the Start page click Getting Started with BlueNotes420 for a pdf or to start the presentation 1 7 Warranty Full warranty terms are listed in Appendix to this manual ...

Страница 18: ...d the maximum relative hum idity must not be more than 85 non condensing 2 2 Services Required Electrical Supply An A C supply between 90V and 240V 10 at 50Hz or 60Hz is required for the Model 420 and 425 The instrument is powered from an external power supply which will accommodate these voltages without any need to adjust the instrument Fuel A supply of Propane Butane or Propane Butane mixture r...

Страница 19: ...420 Universal external power supply 1 100 99 010 Nebuliser cleaning wire pack of 3 1 pack 001 31 076 O ring spare for mixing chamber 1 420 08 102 Nebuliser 1 400 22 003 Nebuliser inlet tube polythene 150 mm 1 425 91 001 Operators manual M420 M425 1 926 09 052 RS232 Output Cable 1 001 08 718 Unex tubing clamp 4 001 56 100 Standard Solution containing l40 0mmol l Na 5 00mmol l K and 1 5mmol l Li 1 x...

Страница 20: ...op of the chimney 3 Remove the Nebuliser 420 08 102 from its box and push the barbed connector into the end of the air tubing item 3 figure 2 1 Fit the Nebuliser item 1 figure 2 1 to the mixing chamber and position the retainer item 2 figure 2 1 to lock it into position Locate the Nebuliser inlet tubing 400 22 003 and fit over the narrow tube in the front of the Nebuliser Figure 2 1 Mixing Chamber...

Страница 21: ...or 5 Gas Regulator inlet connector 5 Turn on the fuel supply and check all connectors for leaks using soap solution Do not use the instrument until you are satisfied that the installation is leak proof 6 Connect the air compressor to a suitable mains supply 7 Connect the length of reinforced hose 001 72 043 between the air compressor outlet and the air inlet connector on the rear panel item 2 figu...

Страница 22: ... and drain If necessary connect a suitable length of tubing not supplied to extend the drain tubing to carry waste to a sink or other drain receptacle The downward flow of waste must not be restricted 9 Make sure that the constant head and drain is pushed fully down on its mounting screws Use a wash bottle item 1 figure 2 4 to fill the U tube with deionised water Sufficient water should be used to...

Страница 23: ...Power supply CAUTION Ensure that the green yellow earth connector in the a c supply lead is connected to a properly grounded earth point the brown lead is connected to the Line terminal L and blue lead is connected to the Neutral terminal N 12 If necessary set the 1 0 power switch below the chimney to the 0 position and connect the plug to a convenient supply socket 13 If no printer computer or ch...

Страница 24: ...n This program can be downloaded from the Sherwood Scientific Website www sherwood scientific com A computer connected to the instrument with the serial cable or via a USB to Serial Adapter not supplied and a serial cable is required to run the BlueNotes for 420 application offering additional facilities See Section 1 6 Analogue Devices The Model 420 Model 425 have one set of analogue outputs corr...

Страница 25: ...splay day as d 10 Adjust with or Press Set to confirm and move on Display month as n 05 Adjust with or Press Set to confirm and move on Display year as y 98 Adjust with or Press Set to confirm and move on Display hour as h 13 Adjust with or Press Set to confirm and move on Display minutes as n 40 Adjust with or Press Set to confirm and move on Automatically sends output as in format day 10 month 0...

Страница 26: ...ds the top display will show 1000 and the lower display will show F1 2 Adjust the top display using the and controls to the value for the Sodium Na filter Press Set in the top display to fix the value This will then reveal 1000 in the top display and F2 in the lower display 3 Repeat step 2 for F2 Potassium K value 4 Repeat step 2 for F3 Lithium Li value 5 Repeat step 2 for F4 Calcium Ca value 2 8 ...

Страница 27: ...on Channel 1 Which allows the output voltage to be adjusted see Section 2 10 below NB It is essential while operating in Peak Mode to aspirate the standard or sample before pressing Cal or Print to take advantage of this facility 2 10 Setting the Output Voltage The output voltage is the maximum voltage measured when at full scale This can be set from 0 05 to 7 5 1 Following the setting or acceptan...

Страница 28: ...h droplet of water evaporates leaving a solid core of the residue of evaporation The core further breaks down to the molecular level and provided the molecules are not too refractory progress to form atomic species The atom then is excited by the flame and its electron temporarily moves to a higher energy state When the electrons return to the ground state they lose the excitation energy and a dis...

Страница 29: ...er uptake rate etc It works by adding a known concentration Co of Li to the Blank and the diluent of the Standard solutions and the Sample The Li signal is constant at Co if all the external effects are constant If the Li signal changes it does so because of flame and instrument variables By introducing a factor based on the reference Li measurement we can remove many of these variables from the a...

Страница 30: ...nt Ranges Model 420 Model 425 Elements analysed Na K with Li Int Std Li Na K Ca with Li Int Std Li Readout No Units or mmol l No Units or mmol l Sensitivity for 100 displayed Na and K 0 5ppm Li 2 ppm Na and K 0 5ppm Li 2 ppm Ca 5ppm Reproducibility 1 1 Reading Stabilisation Variable software command Variable software command Recorder output 2 outputs 50mV 7 5V via software command 2 outputs 50mV 7...

Страница 31: ...alcium there is a significant background interference from Sodium The emission of a Calcium solution will be increased in the presence of Sodium in the solution Contact Sherwood Scientific for methods for accounting for the interference 4 4 Accuracy Linearity Better than 1 measured at mid range when standardised at the following concentrations 140 0mmol l Na 5 0mmol l K 1 5mmol l Li diluted 1 in 2...

Страница 32: ...s before dilution Dilution Ratios Na K 1 200 Serum Na K 1 5 Urine Li 1 50 Type Is dependent on the application However samples should not be highly viscous or non homogeneous Method of Presentation The diluted sample is presented to the nebuliser from a sample cup test tube or other suitable container Alternatively a continuous flow diluter Model 805 can be used and the undiluted sample can be pre...

Страница 33: ... 35 C with a maximum of 7 C shift during 8 hours Humidity Operating 85 maximum at 35 C Transportation 95 maximum at 45 C non condensing For use in these conditions use the Compressor Model 855 with a water cooled trap 4 9 Power Requirements Voltage 90V to 132V or 198V to 264V 50 60 Hz Power 20 VA 4 10 Fuel Propane Butane or Propane Butane mixture Not Natural Gas as the Model 420 425 features a ful...

Страница 34: ...odel 425 especially at high gain depends on a smooth aspiration of the sample this can be disturbed by droplets of condensation coming from the air compressor where the compression expansion causes significant condensation in humid atmospheres If condensation problems arise a Model 855 Air Compressor should be used which has a water separator fitted Air Compressors are listed in Section 9 2 4 12 S...

Страница 35: ...uous in various combinations Measure Print Initiates Measure cycle in Peak Mode Initiates Print in Continuous Mode Enter set up mode for Date and Time Enter set up Mode for Filter factor Channel 1 Set Initiates Calibration procedure Confirms Calibration Value Confirms Set up choices Channel 2 Set Initiates Calibration procedure Confirms Calibration Value Enter Set up Mode for Filter Factor Softwar...

Страница 36: ...king mode and into Set up mode Single Dual and Element Selection Used at start up to choose Single or Dual channel operation Used to select particular elements by toggling through the sequence The Model 420 The Model 425 Na K Na K Na Li Na Ca Na Na Li K Ca K Li Ca Li Na Ca K Li When in Ref Mode with Li Internal Standard Li cannot be measured and will not be selectable Peak Ref Cont Used at start u...

Страница 37: ... mounted behind a flexible plastic overlay which provides protection from the laboratory environment Some windows are plain rectangles others are text legends Cal Ch1 Ch2 Cal on a given channel is illuminated when the instrument automatically goes into calibrate mode after a successful blanking Cal Starts flashing after the Calibration sequence has been initiated Cal changes from flashing to stead...

Страница 38: ...ime Measure Indicates that the instrument is in Measurement mode Measure flashes during Peak measurement process before completion Flame On Indicates that an internal sensor has detected the presence of the flame absence of illumination after the ignition process will be accompanied by the error message E61 on the measurement displays Power On Indicates that the instrument is on and connected to t...

Страница 39: ...plied should be connected here The other end of the cable is a 9 pin standard RS232 connector for serial printers and other data logging devices External Device Output This is another 8 pin DIN connector which enables the instrument to be connected to the Model 860 Autosampler Power The Model 420 and Model 425 are supplied with an Universal External Power supply which can accommodate 90 240V AC in...

Страница 40: ...d Ch2 to access K or Li For the Model 425 there are 4 detectors the additional detector is for Calcium which can be selected as a single element Dual This mode allows the user to simultaneously measure two elements in the same sample For clinical use this guarantees better than a 100 time saving compared with a single channel instrument Ch1 will display Na Ch2 can be either K or Li unless in Ref m...

Страница 41: ...mple quickly followed by pressing Measure The instrument starts a built in delay which can be varied see Section 2 9 as soon as a press of Measure has been registered whether a short long or double press does not matter readings are displayed on all operative channels When the values satisfy the peak detection algorithm the display s freeze and the Measure indicator will become steady A single bee...

Страница 42: ...ode can be used in various combinations with Single Dual and Ref There is one major difference from the Peak mode a press of Print causes the output of the current displayed reading to whatever is connected to the RS232 connector e g printer computer data logger 5 3 4 Blank Blanking can be initiated at any time during a measurement session by pressing Blank having already introduced a blank sample...

Страница 43: ...r even three operations might be necessary to fill the gas tube up stream of the instrument 6 1 3 Ignition Successful The instrument automatically selects a reduced gas air mixture which optimises the flame for highest sensitivity The Flame detector operates and the flame on light illuminates 6 2 1 Required Blanking When the instrument has just been switched on it automatically goes into blanking ...

Страница 44: ...ch what the user judges to be steady plateau values and presses Set on each channel at the appropriate time to confirm the values As these presses of Set are registered the displayed readings are adjusted to calibration values the output is sent and the instrument goes into measure mode with Measure on and Cal off Peak Mode With Reference is Default Mode In Dual Mode both Sodium and Potassium stan...

Страница 45: ...ducing the sample the user will decide when the displayed results have stabilised and the reading can be taken and or sent to the printer output When he she is so satisfied then a simple press of the Measure Print button will send the currently displayed measured value to the output port A new sample can be presented at any time and the user again can take the result when it is judged ready 6 4 Au...

Страница 46: ...3 QC sample A Quality Control sample can be identified by a long push on the print button the output is then QQQ and the next sample is not incremented To confirm that the instrument has accepted these different pushes on the Measure button the measurement display will disappear for two seconds during which time the second push will be accepted 6 6 Shutdown Procedure 1 Aspirate Cleaning Solution d...

Страница 47: ...part Diluent Concentrate and 999 parts of good quality distilled water which contains non ionic wetting agents 2 The samples should not be highly viscous or non homogeneous If possible samples likely to contain sediment should be filtered and then mixed to obtain a representative result 3 Always use soap solution when checking for leaks in fuel or air lines Do not allow fuel to flow in the presenc...

Страница 48: ...on a supposedly empty cylinder 3 The chimney cap and glass chimney and the area above the chimney can become very hot and are capable of causing severe burns Never view the flame from the top of the chimney always use the inspection hole 4 Make sure that the air compressor is connected to the power supply and switched on before starting a flame ignition sequence If this is not done the instrument ...

Страница 49: ...r line for condensation and drain if necessary 3 Check U tube is filled with water 8 3 Weekly Maintenance EQUIPMENT REQUIRED 10ml beaker Stopwatch Nebuliser cleaning wire Deproteinising Solution Stainless steel nebuliser tube and sleeve or polythene inlet tube as required 1 Carry out Daily Maintenance procedure 2 Deproteinise the system see Section 8 10 3 Check the operation of the nebuliser see S...

Страница 50: ...ng and connectors for leaks using soap solution 8 6 Nebuliser EQUIPMENT REQUIRED Stopwatch 10ml beaker Cleaning wire Cleaning Solution Polythene inlet tube 8 6 1 Operational Check 1 Turn off the fuel supply at source 2 Switch on the instrument and the air compressor Check that the flame on LED is off 3 Fill the beaker with deionised water and weigh it 4 Present the 10ml beaker of deionised water t...

Страница 51: ... particles are apparent 9 With the Nebuliser Inlet Tube removed but with the Nebuliser attached to the Air Tubing place a finger over the Nebuliser Outlet Nozzle turn on the air supply to force any residue out of the Nebuliser s capillary tube 10 Refit the nebuliser inlet tubing to the nebuliser Recheck the aspiration rate paragraphs 3 4 and 5 11 If nebuliser operation is still outside the specifi...

Страница 52: ...water in the U tube to flow into the constant head and drain Disconnect the U tube from the end cap and constant head and drain 8 Unclip the constant head and drain and disconnect the drain tube 9 Soak all the items removed from the instrument in a 1 in 100 dilution of Cleaning Solution agitating it periodically 10 Rinse the parts thoroughly in distilled water and dry with clean tissues 11 Fit the...

Страница 53: ...move the two screws at the rear of the chimney underneath the flange and the two thumbscrews under the front of the chimney 6 Lift the chimney assembly up slightly before leaning the top forwards Balance the bottom on the chimney tray 7 Remove the two screws holding the filter detector housing together and to the mounting bracket located on the plinth Then gently ease the two halves apart and remo...

Страница 54: ...rm 35 tap water 2 Light the flame as detailed in Section 5 4 3 Present a beaker of Enzymatic Solution or Tubing Disinfectant to the nebuliser for 5 minutes 4 After 5 minutes have elapsed replace the beaker of the solution with a beaker of deionised water Aspirate for two minutes Then with a fresh beaker of deionized water aspirate for a further 10 minutes 5 Shutdown the instrument as detailed in S...

Страница 55: ... and burner 1 402 12 001 End Cap Assembly 1 400 02 013 End cap securing screw 1 401 11 001 Constant Head Drain moulded Large 1 400 22 003 Nebuliser inlet tube polythene 150mm each 1 400 22 012 Nebuliser inlet tube polythene 150mm Pk 12 1 420 08 022 Fuel restrictor 22 g 1 420 08 023 Fuel restrictor 23 g 1 001 26 033 Sample pot plastic in packs of 50 1 pack 420 27 137 Sodium filter Factored 1 420 27...

Страница 56: ...r separator 1 9 3 Reagents Catalogue Description Quantity 001 56 100 140 0mmol Na 5 00mmol K 1 50mmol L Li 100ml 1 Bottle NOTE The following solutions are also available 001 56 620 Flame Photometer Standard 1000ppm Na 6 x 100ml 1 Pack 001 56 621 Flame Photometer Standard 1000ppm K 6 x 100ml 1 Pack 001 56 622 Flame Photometer Standard 1000ppm Li 6 x 100ml 1 Pack 001 56 623 Flame Photometer Standard...

Страница 57: ...Hald P M J Biol Chem 167 499 1947 4 Bernstein R E S Afr J Med Sci 17 101 1952 5 Alkemade C T J Smit J and Verschure J C M Biochimica et Biophysica Acta 8 562 1952 6 Collins G C and Polkinthorne H The Analyst 77 917 pp 430 436 1952 7 Dryer R L Clin Chem 2 112 1956 8 Puffeles M and Nessim N E The Analyst 82 976 1957 9 Boling E A J Lab and Clin Med 63 501 1964 10 Amdisen A Scand J Clin and Lab Invest...

Страница 58: ...take rate E12 is displayed for 5s then automatically returns to start of calibrate does not go back to beginning of blanking No output sent E15 Calibration Invalid Reading Calibration Factor 99 Check concentration of Standard has it been diluted correctly Start Calibration sequence again E16 Detector circuit saturated in Calibrate Gross error in Standard concentration Standard not diluted Check co...

Страница 59: ...bing Check nebuliser E35 is displayed on active channels and Ref flashes for 5s then automatically returns to start of blanking Aspirate a blank solution and press Blank E39 Li signal outside limits in calibrate In Ref mode only E39 is displayed on Ch2 and Ref flashes Li Concentration in the final Standard solution should be 100ppm Check concentration of sample Increase sample dilution E39 is disp...

Страница 60: ...lows the damping to be changed see section 2 11 in Operator manual Press Set 4 Display reverts to the blank setting 000 flashing on each channel Operation of Zero Offset 1 Aspirate blank solution and press Blank 2 When blank is captured after 5 seconds the Cal lights flash 3 Operate the or keys to change the output analogue voltage 4 Press Set Operation of the Span 1 Std will be displayed Aspirate...

Страница 61: ...ivery of product to end user premises 2 Free of all charges statement applies only in areas recognised by Sherwood as being serviced either directly by its own personnel or indirectly through personnel of an authorised distributor Products purchased outside these areas requiring service during the warranty period will incur charges relative to the travel transit costs involved However products pur...

Страница 62: ...warranties purporting to be on behalf of Sherwood made by any person including distributors and representatives of Sherwood which are inconsistent or in conflict with the terms of this warranty including but not limited to the limitations of the liability of Sherwood as set forth above shall not be binding upon Sherwood unless reduced to writing and approved by an officer of Sherwood Scientific Lt...

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