Model 420 & 425 Flame Photometer
425 91 001
Issue 22
1
st
December 2016
ECN 1410 & 1932
- 11 -
Introduction
continued
1.5
Dilutions
continued
Sample
type
Instrument
mode
Blank solution
Standard solution
preparation
Sample
preparation
Na & K
in
Serum
Clinical Mode
101 with Ref
facility
1 part diluent
concentrate to 999
parts 100ppm Li
solution
Sherwood Clinical
standard diluted
200 times with the
blank solution.
Dilute sample
200 times
with the blank
solution.
Clinical Mode
101
1 part diluent
concentrate to 999
parts good quality
deionised water
Sherwood Clinical standard prior to dilution contains 140.0mmol/l Na & 5.00mmol/l
K, therefore, set the calibration figures: Na to 140.0 & K to 5.00. As the samples
underwent the same dilution as the standard the display shows the mmol/l results in
the original sample.
Printed results show mmol/l units
Sample
type
Instrument
mode
Blank solution
Standard solution
preparation
Sample
preparation
Na & K
in Urine
Clinical Mode
102 with Ref
facility
1 part diluent
concentrate to 999
parts 100ppm Li
solution
160mmol/l Na &
80mmol/l K
standard diluted
one part in five
then 200 times
with the blank
solution.
Dilute sample
one part in
five then 200
times with the
blank
solution.
Clinical Mode
102
1 part diluent
concentrate to 999
parts good quality
deionised water
The standard contained 160mmol/l Na & 80mmol/l K prior to dilution, therefore, set
the calibration figures: Na to 160.0 & K to 80.0. As the samples underwent the same
dilution as the standard the display shows the mmol/l results in original sample
Printed results show mmol/l units
Sample
type
Instrument
mode
Blank solution
Standard solution
preparation
Sample
preparation
Li in
serum
Clinical Mode
101 or 102
1 part diluent
concentrate to 999
parts good quality
deionised water
5.0mmol/l Li
standard diluted 50
times with the
blank solution.
Dilute sample
50 times with
the blank
solution.
The standard contained 5mmol/l Li prior to dilution, therefore, set the calibration
figures: Li to 5.00. As samples underwent the same dilution as the standard the
display shows the mmol/l results in the original sample.
Printed results show mmol/l units
Table 1.5.2 – Dilutions of Standards