Sartorius stedim Sartocon ECO Series, Руководство пользователя, Страница: 15 / 40

15 Start up of cassettes
2.6 Filtration
A critical time during crossflow filtration is start up. The process fluid to be filtered
initially contacts the clean membrane surface. Until the desired steady state process-
ing conditions are attained, a higher than expected permeate flux rate will prevail.
Please consider:
t
Partly close permeate valve to limit permeate flow.
Starting a Crossflow filtration process:
1. Prime the permeate and retentate areas with one of the liquids listed below:
t
Isotonic physiological saline
t
Processing buffer
To prime the system using one of the liquids listed above, follow the directions given
in the section entitled
”Flushing the Filter Cassettes”
section 2.3.
2. Reduce permeate outlet valves setting to allow a small amount of fluid to exit.
3. Generate the desired crossflow rate. The inlet pressure will increase.
4. Open the permeate outlet valve until the desired TMP is attained.
5. Make sure that the desired crossflow rate for the process is maintained.
6. Reset the desired process TMP if required.
The pressure at the feed inlet p
inlet
must not exceed 58 psi|4 bar.
The maximum allowable backpressure for all Sartocon
®
ECO cassettes is:
<
7 psi|0.5 bar|50 kPa.
During filtration, the crossflow rate should be held constant. Viscosity increase
during filtration will increase the inlet pressure.
7. At the end of filtration, flush the system (see ”Flushing the Filter Cassettes” ).
8. After flushing, clean the system (see ”Cleaning the Filter Cassettes” ).