Sartorius Stedim Biotech BIOSTAT CultiBag RM 600, Руководство по эксплуатации, Страница: 6 / 36

1. Structure and Function
1.1 Usage of BIOSTAT ® CultiBag RM 600 The BIOSTAT ® CultiBag RM 600 is a large scale rocking platform for cultivation of cells
and microbes in Disposable Bag Bioreactors (CultiBag RM 600). The Rocking platform
is combined with the BIOSTAT
® RM Control Tower which allows monitoring and
control for pH, DO, temperature, rocking angle and rocking rate.
BioPat
® MFCS | DA software for external data logging is included.
Rocking motion mixing technology utilizes mechanical energy to ensure homoge-
neous mixing of cells with low shear. Energy input is effected by rocking the CultiBag
RM 600 side to side, generating a fluid movement in the cell culture and medium.
In this way the surface of the medium is continuously renewed for bubble-free
surface aeration.
CultiBag RM 600 disposables bag for cultivations of up to 300 L of culture volume.
The rocking platform, Rocker 600, is operated via the Control Tower.
All set points and control functions will be controlled and displayed in the BIOSTAT
®
RM Control Tower.
For further information regarding connection of the Rocker 600 to the BIOSTAT
® RM
Control Tower see associated manuals for Control Tower.
1.2 CultiBag RM 600 Cell cultivation is performed inside the CultiBag RM 600 disposable bioreactor
chambers, which are specially designed to work with the BIOSTAT
® CultiBag RM 600.
These bags are delivered presterilized and ready for use. The CultiBag RM 600 is
produced of multilayer film, USP class VI and is designed for single use operations.
This feature eliminates the need for cleaning and sterilizing the bioreactor with
considerable savings in labour, no danger of cross-contamination and greater
assurance of sterile operation.
CultiBag RM 600L optical, mounted on table
1 Fill port, C-Flex tubing with MPC coupling
2 Spare port with luer connector
3 Sample port, with luer-septum for sampling or inoculation
4 Fill | drain port, C-Flex tubing with Luer
5 Air outlet sterile filter
6 pH and DO probes
7 Fill port, C-Flex tubing with MPC coupling
CultiBag RM 600L only are recommended for use with the BIOSTAT ® CultiBag RM 600.
6 Structure and Function
Fig 1: Set Up of BIOSTAT
®
CultiBag RM 600
optical
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