5. Cultivation
5.1
Preparing for the Cultivation
5.1.1 Filling the CultiBag RM 600 with culture media
For information regarding use of the intuitive Touch screen, please refer to the
associated manual for BIOSTAT
®
RM Control Tower.
The CultiBag RM 600 should be inflated prior to filling with media, see section
Inflation of the CultiBag RM 600. This will reduce creases and reduce foaming.
Continue air flow and activate the pressure control loop to the headspace to keep
the bag rigidly inflated, whilst filling with media.
Do not rock while filling.
1. Aseptically, connect tubing from the media container to the CultiBag RM 600
chamber using BioWelder, BioSealer, luer fittings or MPC couplings to connect
two tubes together.
Guide the tubes through the openings on the left and right sides of the rocking table.
Use of luer and MPC couplings may require moving the CultiBag RM 600 Bag into
a bio safety laminar flow cabinet for aseptic connection. The sampling luer may
also be used.
2. Pump the desired volume of media into the bioreactor, and then aseptically
disconnect the CultiBag RM 600 from the media container.
Do not use any tools to connect the CultiBag RM to avoid damaging the bag or tubes.
Avoid any folds at the tubes and do not squeeze the tubes.
Do not start rocking motion while the lid of the Rocker is opened. Risk of injury.
Ensure that both valves mounted at inlet and outlet filter are open. Also ensure
the filter heaters are mounted correctly.
If the valves of the air filters are closed or are not connected while filling the bag
with media, may result in overpressure inside the bag causing rupture of the bag.
3. Close the lid and lock all closures.
Cultivation
23
NOTICE