8. Supplement
8.1
Optimize Wave Motion
Every cell line requires optimization of operating conditions. The attached graphs
reflect a guide line. Tests estimating optimum formation of wave mixing are required.
Specification of the CultiBag RM 600L optical:
Min. working volume: 60 L
Max. working volume: 300 L
Legend
Minimal wave forces, below limit.
Minimal oxygen transfer, no shear forces
Low wave formation, no forming, minimal wave forces.
Low oxygen transfer, minimal shear forces
Ideal wave formation, no framing, minimal shear forces.
Well oxygen transfer, minimal shear forces
Well wave formation, slight foaming, small shear forces.
Very well oxygen transfer, small shear forces
Heavy wave formation with foaming, middle shear forces.
Very well oxygen transfer, heavy shear forces
To heavy wave formation, heavy foaming, heavy shear forces.
Very well oxygen transfer, to heavy shear forces
30
Supplement
Angle
Rocking rate [Rocks/min]
[°]
4
6
8
10 12 14 16
10
8
6
4
CultiBag RM 600L optical:
Working volume: 60L
Angle
Rocking rate [Rocks/min]
[°]
4
6
8
10 12 14 16
10
8
6
4
CultiBag RM 600L optical:
Working volume: 150L
Angle
Rocking rate [Rocks/min]
[°]
4
6
8
10 12 14 16
10
8
6
4
CultiBag RM 600L optical:
Working volume: 240L
Angle
Rocking rate [Rocks/min]
[°]
4
6
8
10 12 14 16
10
8
6
4
CultiBag RM 600L optical:
Working volume: 300L