Tissue
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Quick Reference Guide
Tissue-Tek® Xpress® x120
page 8 | 10
QRG | x120 | 2010 | V-10
Monthly
1.
Fume control system, filter replacement when
x120 is not connected to an external hose
system.
Fume control system
Replace carbon filters
Service maintenance
1.
Overflow bottles: once a year during
service maintenance.
Overflow bottles
Reprocessing of Tissue
Tissue-Tek Xpress x120-processed tissue may be reprocessed, if necessary, without adverse
effects on the tissue. Tissue that has been trimmed too thick for proper processing can be
reprocessed using the following reprocessing procedure.
Procedure:
1.
Place tissue cassette in a 60° C oven or in the Tissue Embedding Center and allow the block
to melt down.
2.
Remove tissue and place in a cassette identified with the proper accession number.
3.
It is important to note the thickness of the residual tissue.
4.
Place the labeled cassette in solvent (xylene or xylene substitute) for 30 minutes (two
changes).
5.
Place the cassette with tissue in a beaker containing absolute alcohol for 30 minutes (two
changes).
6.
Remove the cassettes from the alcohol.
7.
If necessary, re-trim the tissue to the correct thickness.
8.
Place the tissue in Pre-Processing solution for 15 minutes.
9.
Remove the cassettes from the Pre-Processing solution and place in a processing basket.
10.
Depending on the thickness of the residual tissue, select the appropriate immersion program
(2mm or 3mm).
11.
Place the processing basket in the Loading Station.
12.
Enter the number of cassettes loaded, as required.
13.
At the completion of the processing program, remove the processing basket.
