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Phoenix HT User Manual

© 2007–2012 Rigaku Automation, Inc.

32

401718 Rev A

2

Twist and disconnect the pneumatic tubing from the nano dispense controller.

8. Carefully disconnect the nanotrigger wire from the needle tip by pulling on the white

connector which also holds the nano-tip in place and remove the nano-tip from the nano-tip
sleeve.

9. Remove the entire nano valve harness.

Twist and disconnect the
pneumatic tubing.

Nano trigger wire clip

Nano-tip assembly

Nano-tip sleeve

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Содержание Phoenix HT

Страница 1: ...Phoenix HT User Manual May 2012 401718 Rev A TM...

Страница 2: ...lity for any errors or omissions nor for any damages resulting from the application or use of this information Except as specifically set forth in its terms and conditions of sale Rigaku makes no warr...

Страница 3: ...T System from Cold to Warm Areas 9 1 5 Rigaku Service and Support 9 CHAPTER 2 BASICS 11 2 1 System Power On 11 2 2 Shutting Down the System 12 2 3 Running the System 12 2 4 Status Light Indicators 12...

Страница 4: ...5 3 Daily Detergent Wash Required 28 5 4 Replacing the Nano Tip Valve Harness Assembly 30 5 4 1 Removing the Nano Tip Valve Harness 30 5 4 2 Installing the Replacement Nano Valve Harness 33 5 5 Calibr...

Страница 5: ...vie s rieuse WARNING WARNING alarmieren Sie zu den Situationen die ernste Personensch den oder Verlust oder Lebensdauer ergeben konnten ALERTA Las alertas le alertan a las situaciones que podr an dar...

Страница 6: ...d in the lab NaCl is a chemical 5M NaCl is a stock A default stock is generally the concentration of the aqueous solution of a chemical that is sold by Hampton Research A reservoir design is a collect...

Страница 7: ...xperimental plates using screens contained in deep well blocks and protein samples A dispense head containing 96 syringes draws specific volumes of chemicals from a deep well block and places them in...

Страница 8: ...n an experimental plate An antistatic curtain to reduce static electricity that can interfere with drop placement A protein chill block to chill vials of protein or other substances Dual chimney wash...

Страница 9: ...king Red Power is on software launched system has an error condition and system has stopped or the system requires initialization or the EMO is active Not initialized EMO pressed liquid handling error...

Страница 10: ...r Block Slots Figure 6 Temperature Controller Front Panel Figure 7 Temperature Controller Rear Panel To apply power to the Protein Chiller Block flip the rocker switch on the back of the box labeled T...

Страница 11: ...e three lines are controlled by three valves Located on the right side of the water pump control box push buttons prime the pumps a process that removes air in the lines and allows free water flow Was...

Страница 12: ...se nano liter drops of protein or other samples Figure 11 Nano Dispense Controller Front 96 head wash station chimney Nano wash station chimney Supply line to the nano dispenser tip Switching valve be...

Страница 13: ...tive Nest positions a plate against the indexing pins under software control for accurate dispensing The shelf must be in the front position and contacting the electrical pins in order to complete the...

Страница 14: ...Shelf Position Sensors 1 2 9 Active Nest Terminals The Active Nests if installed can receive commands only when the terminals on the shelf are in contact with the terminals on the front of the base u...

Страница 15: ...network connection for the Phoenix system through the Database Server The control computer is connected via Ethernet to the Database Server 1 4 2 Electric Power Hazards The Phoenix HT system operates...

Страница 16: ...nline service request form Located on http www rigaku com contact service htm If the system fails note what happened any messages that appear on the monitor lamp displays what the system was doing bef...

Страница 17: ...Cabling on page 40 2 1 System Power On on page 11 2 2 Shutting Down the System on page 12 2 3 Running the System on page 12 2 4 Status Light Indicators on page 12 2 5 Using the Stop Start and Emergen...

Страница 18: ...ition Machine Specific All Off System has no power Solid Red Power is on software launched software in stopped state or machine is not ready to process orders Machine can not start processing orders S...

Страница 19: ...process 5 Perform a wash 6 In the Jobs View select the job that was running when the EMO button was pushed 7 Select Jobs from the menu bar and select Edit Job Check the Reset job status check box to...

Страница 20: ...re IMPORTANT Be sure that the cap to the tube is removed before inserting the tube into the chill block Failure to do this can result in damage to the nano dispenser needle 2 If you are not using the...

Страница 21: ...Open the Rigaku Phoenix application and click the Use Automation checkbox The connection checks the Integration Module s inventory for the materials required to execute the job Empty crystallization p...

Страница 22: ...orner of the window and click OK when a message to register additives appears 4 For each sample of the three samples in this example job select the appropriate entry for each field then click OK Block...

Страница 23: ...below example the DEPLETED message indicates a shortage of liquid for Sample C in subwell 3 Note that the Job Status is In Process and the Task Status is Supplies 6 To fix the supply problem click on...

Страница 24: ...o to the Jobs view select the Task and click Load 2 Click the Green Run button at the top of the window to run one task or job or To automatically run a series of selected jobs and tasks click the Aut...

Страница 25: ...21 Views Pane and Icons on page 23 4 1 Organization The Phoenix HT application is organized into groups of related functions The main operator interface contains menus integration controls run control...

Страница 26: ...2 View Menu 4 4 Jobs Menu The Jobs menu contains selections adding editing deleting displaying and running jobs Figure 3 Jobs Menu Add New Job Opens the New Job dialog to add a job to the list Click t...

Страница 27: ...s Check to allow you to select multiple jobs in the Jobs view Auto Run by Protein Order Runs the jobs in the list in order of the proteins Refresh Jobs Displays the current jobs in the active view 4 5...

Страница 28: ...ther sections Toggle Top Active Nest Toggles the active nests in the upper shelf from the current state If the active nests were closed this selection opens the active nests The shelf must be in the f...

Страница 29: ...lete box to purge jobs from the active view and save them in a history folder Figure 11 Options Dialog 4 6 Help Menu The Help menu contains the About selection to display the version number of the Pho...

Страница 30: ...ed Block and Ambient Block Tabs The Chilled Block tab and the Ambient Block tabs display the samples in the chilled block and the ambient block The Chilled Block tab and the Ambient Block tab display...

Страница 31: ...ck or Ambient Block The dialog contains the same controls and text boxes as the Add Sample dialog Remove Removes the selected samples Clear All Removes all samples in the list 4 9 2 Onboard Plates Tab...

Страница 32: ...uttons on the right are Edit Opens the Edit Onboard Plate dialog which allows you to edit the plate barcode Figure 17 Edit Onboard Plate Dialog Remove Removes the selected plates Clear All Removes all...

Страница 33: ...Rev A The menu and buttons on the right are Show History For Opens a menu for the time period of the jobs listed Details Opens a Details pane to show details of the selected job Add to Jobs Opens a di...

Страница 34: ...option in Rigaku Service Contracts 5 2 Equipment Cleaning Use a gentle nonabrasive detergent to clean surfaces of the Phoenix HT instrument If a spill occurs on the equipment stop the current exterior...

Страница 35: ...the detergent wash can be configured in this manner Note the larger dispense volume with the purge selected Because this is a wash the aspirate and dispense occur in the same location Figure 2 Config...

Страница 36: ...the rocker switch on the nano dispenser controller box 3 Turn off the power to the Phoenix instrument at the rocker switch on the back of the main unit on the right hand side 2 Gently push the nano di...

Страница 37: ...hat also holds the harness in place 7 Disconnect the nano dispenser from the nano dispense controller 1 Unsnap and disconnect the black electrical wiring from the nano dispense controller Remove velcr...

Страница 38: ...e controller 8 Carefully disconnect the nanotrigger wire from the needle tip by pulling on the white connector which also holds the nano tip in place and remove the nano tip from the nano tip sleeve 9...

Страница 39: ...harness in it 4 Thread the harness between the two cable guide pins on the nano head 5 Reinstall the second P clamp onto the front of the nano dispenser head and position the harness in it with the ap...

Страница 40: ...ange CHxx H0002 xml to CH01 H0002 xml Make sure the new harness s Hardware Calibration file is located in the same location at Phoenix RobbinsNanoCalibration RobbinsNanoHardwareCalibration While CHXX...

Страница 41: ...tacles or consumables on the tray positions 3 Ensure that the predrilled holes for the accessible tray positions are completely visible Using a flashlight is highly recommended 9 If the previous nano...

Страница 42: ...t also displays the saved location and the current nano tip location The rectangular box with four arrows and the stop in the middle moves the nano tip left and right left and right arrows and moves t...

Страница 43: ...command to shift the drop until the two coincide The below procedure provides additional precision When the nanodispenser tip on the Phoenix is changed its dispense position will shift even when remov...

Страница 44: ...will need to apply this to the rest of the dispense positions 6 Apply offsets to all positions 1 Once you are satisfied with the drop placement on one position the position shift must be applied to a...

Страница 45: ...l flex needle syringe Average replacement cycle 4 syringes per year 4 spare syringes and needles provided with Phoenix DT 205673 100 l Teflon Tips Quantity 100 Average replacement cycle 100 tips per...

Страница 46: ...and electrical layout considerations for the Phoenix HT B 1 Mechanical Layout The following information is current as of the date of publication Contact the Rigaku Automation Service organization for...

Страница 47: ...aku Automation Inc 41 401718 Rev A Figure 45 Power and Network Connections The following figures are components installed in the Phoenix HT enclosure Figure 46 Phoenix Base System Front and Top Dimens...

Страница 48: ...Phoenix HT User Manual 2007 2012 Rigaku Automation Inc 42 401718 Rev A Figure 47 Phoenix Side Depth Dimensions and Power Requirements Figure 48 PC Power Requirements...

Страница 49: ...07 2012 Rigaku Automation Inc 43 401718 Rev A Figure 49 Temperature Controller Unit Dimensions and Power Requirements Figure 50 Nano Dispense Controller Dimensions and Power Requirements Top View Side...

Страница 50: ...em Cabling The following connections must be taken to properly set up the system This does not need to be repeated unless the equipment has been disconnected Connect the cables to the Facilities Panel...

Страница 51: ...anel of base system Water Pump Control Box Supply Tank Waste Water Tank Nano Trigger wire Trigger RS 422 Nano Wash Head Wash USB USB Temperature Control Unit blk red grn wht brn blu TEC FAN Sensor To...

Страница 52: ...gaku Automation Inc 46 401718 Rev A Figure 53 Phoenix HT Water Connections Phoenix water connections underside of base system Water supply Plumbed Water Control Module Waste water Plumbed Nano wash He...

Страница 53: ...on Phoenix HT enclosure contains the following additional 250 volt slow acting fuses 120 V 240 V Line Neutral 8 A 8 A 8 A Line Neutral Phoenix base system AC Line FU 2 3 15 A Nano Dispense Controller...

Страница 54: ...e Date Revision Description of change 05 30 12 A Complete rework of and update to Phoenix RE user manual DOO1457 published in 2008 separated into Phoenix HT User Manual and Phoenix DT Supplement manua...

Страница 55: ...hanging drop vi hardware parts 39 hazards 9 Help menu 23 J Job History view 26 Jobs menu 20 Jobs view 23 L layout mechanical 40 light indicators 12 Logging command 22 M Machine Control 22 main window...

Страница 56: ...the system 12 product description 1 product options 2 protein chiller block adjusting temperature 4 protein chiller block slot locations 3 R recipe vi Refresh DB Info 22 Refresh Jobs 21 Register Samp...

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