4040+e_22.docx / 06.10.17
ROBERT RIELE GmbH & Co KG
Page 39
6.4.14 Calculation procedure 14 (C/F Delta)
Method at which a difference of sample E2
– E1 is measured several times depending on the quantity of samples.
In the first course the samples E1 (maximum 25) will be measured, optionally with or without sample blank. After
a user defined measure time the samples E2 will be measured in a second course. Attention should be paid to
the order within the series to avoid errors. The procedure corresponds to a fixed time kinetic.
Quality control samples can not be saved.
This calculation procedure has special parameters that allow a time controlled measuring process. These
parameters are: time/delta T1, measure time T2, delay T3, reagent time #2 and reagent time #3. By setting a
time/delta (value between 10s and 255s) the other parameters for the time controlled measuring will be used. In
the time controlled mode the quantity of samples is determined by the measure time and the time/delta, e.g. with
a measure time of 60s and a time/delta of 10s it is possible to measure 6 samples (without sample blank). The
measure time should be chosen greater or equal the time/delta.
At the beginning of the method the use of a sample blank is queried.
After the blank is measured the time controlled measuring process will be started with [RESULT]. With a
combination of acoustic signals and text messages on the display the Photometer takes control of the timing for
the whole measuring. The measurement of the samples E1 can be finished at any time with [E1/E2]. In the second
course will be so many samples measured as in the first course.
Before starting a new E1/E2 course a new zero measurement has to be done.
The reagent time #3 is usable only if the reagent time #2 is set. In that case the reagent mode will be entered, i.e.
the photometer also takes control of the timing for dispensing the reagent before measuring the samples and the
quantity of samples will be determined by the reagent time #2 and the time/delta. The reagent time #2 should be
chosen smaller or equal the reagent time #3 and greater or equal the time/delta.
Figure 5.4.14.1 shows the time sequence of a time controlled measuring process with N samples, a delay time
T3 and without reagent time.
E
11
meas.
T2
T1
T1
T1
T1
T3
...
...
Insert
E
11
Insert
E
12
Insert
E
13
E
12
meas.
Press
Result
E
1N
meas.
E
21
meas.
E
22
meas.
E
2N
meas.
Insert
E
21
Insert
E
22
Insert
E
23
t
t:
Time
T1:
Time/Delta
T2:
Measure time
T3:
Delay, optional
A:
Start of time controlled measuring
E1:
Start of first course (E1)
E2:
Start of second course (E2)
Blank-
measure
...
E
1
E
2
A
: automatic process
: manual process
Fig. 6.4.14.1: time controlled measuring