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Spectrum® KR2

/ KMP

TFF Systems

USER GUIDE AND OPERATING INSTRUCTIONS

Spectrum® KrosFlo® Research 2

TFF System

Spectrum® KrosFlo® MiniKros Pilot

TFF System

Содержание Spectrum KMPi

Страница 1: ...Spectrum KR2i KMPi TFF Systems USER GUIDE AND OPERATING INSTRUCTIONS Spectrum KrosFlo Research 2i TFF System Spectrum KrosFlo MiniKros Pilot i TFF System ...

Страница 2: ...2 years from date of shipment The information contained herein is believed to be accurate and is offered in good faith for the convenience of the user PRODUCTS ARE FURNISHED UPON THE CONDITION THAT THE USER ASSUMES ALL RISKS AND LIABILITIES AND THAT NEITHER THE SELLER NOR MANUFACTURER SHALL BE LIABLE FOR ANY LOSS OR DAMAGE DIRECT OR CONSEQUENTIAL ARISING FROM THE USE OF THESE PRODUCTS Spectrum Kro...

Страница 3: ...7 1 Basic Setup 13 7 2 Manual Mode Setup 15 7 3 C Mode Setup 16 7 4 C D and C D C Mode Setup 16 7 5 C D D C Mode Setup 17 7 6 CFC Mode Setup 18 7 7 CF D C Mode Setup 19 7 8 Ctrl Mode 20 7 9 System Setup Using KF Comm Pump Control Window 20 7 9 1 KF Comm User Interface 20 7 9 2 Entering System Settings 23 7 10 Using Pump Interface 30 7 10 1 Keypad 30 7 10 2 Main Screen 31 7 10 3 Remote Control Of A...

Страница 4: ...nent Setup and Operation 58 10 1 Auxiliary Scales 58 10 1 1 Installation 58 10 2 Auxiliary Pumps 59 10 2 1 Installation 59 10 2 2 Auxiliary Pump Settings 61 10 3 Automatic Backpressure Valve 61 10 3 1 Installation 61 10 3 2 Operation 62 10 3 3 Automatic Backpressure Valve Settings 62 10 4 KONDUiT 62 10 4 1 System Configuration and Major Components 62 10 4 2 Installation 63 10 4 3 Software Firmware...

Страница 5: ...1 6 Worksheets Overview 73 Basic Concepts of Tangential Flow Filtration 74 12 1 Introduction 74 12 2 Concentration 74 12 3 Diafiltration 75 Troubleshooting 75 Replacement and Auxiliary Parts 76 Index 77 ...

Страница 6: ...riming TFF system has low shear is capable of running dry and pumping fluids with high quantities of entrained air has high volumetric efficiency and a motor speed repeatability of 0 1 percent to maximize productivity The KR2i is the ideal system for small volume R D scale microfiltration and ultrafiltration due to its compact footprint and versatility The KMPi has been designed to be used with Sp...

Страница 7: ...the seal with foreign material Failure to observe this precaution may result in damage to the seal and premature failure of the seal No foreign matter should be allowed under the gasket on the back of the front plate or under the heads of the screws Failure to observe this precaution may result in leakage during washdown of the drive CAUTION To avoid electrical shock the power cord protective grou...

Страница 8: ...ds Consult the factory for products suitable for these types of applications ENVIRONMENTAL Waste electrical products should not be disposed of with household PROTECTION waste Please recycle where facilities exist Check with your Local Authority or retailer for recycling advice Specifications KR2i Specifications Output Speed 0 1 to 600 rpm Torque output Maximum 180 oz in 13 kg cm 540 oz in Starting...

Страница 9: ... models 520 oz in 37 4 kg cm 1560 oz in Starting Speed regulation All models Line 0 1 F S Load 0 1 F S Drift 0 1 F S Display All models 128 x 64 LCD w LED Backlight Remote outputs All models Voltage speed output 0 10V DC 1 kΩ min All models Current speed output 0 10 mA 0 600Ω All models Tach output 100 to 6500 Hz 50 duty cycle 10 Hz rpm All models Motor running output N O N C contact closure 1A 28...

Страница 10: ...ltered locations Chemical Resistance Models with stainless steel Exposed material is 316 stainless steel enclosure and hard coat anodized aluminum Models with powder coated Exposed material is polyester coated steel enclosure steel and hard coat anodized aluminum Compliance Conforms to ANSI UL Std 61010 1 Certified to CAN CSA Std C22 2 No 61010 1 This product has been tested to the requirements of...

Страница 11: ...cm 0 1 mS cm 2 to 50 mS cm 5 of reading 50 to 100 mS cm typically 5 of reading Temperature Range 0 70 C Accuracy Better than 0 2 C typically better than 0 1 C Optional UV Output signal 4 20 mA sourcing with 400 ohm maximum at 24 VDC scaled to 0 2 AU with repeatability of 1 of full scale 0 02 AU Typical Response Time 1 second Maximum Zero Shift 2 of full scale 0 040 AU Long Term Output Drift 5 per ...

Страница 12: ...2i TFF system comes with an attached mounting plate for the KMPi Easy Load Pump Head 4 3 Octopus Cables The TFF system comes with two Octopus Cables one with more pins for the Auxiliary Components and one with less pins for the Pressure Transducers On the back of the TFF system are two Interface ports for the Octopus Cables System Parts List KR2i TFF System Part Number Pump Description SYR2 U20 Co...

Страница 13: ... Number Material TFF System Product Contact Surfaces Tubing Reservoir Closures C Flex Pharmapure Reservoirs Polypropylene Disposable Pressure Transducers Polysulfone Plastic Fittings Polypropylene Polysulfone Hollow Fiber Module Module Sold Separately Membrane ME mPES PES or PS Housing Pigmented and Non pigmented Polysulfone Potting Polyurethane Epoxy End caps Pigmented Polysulfone Setup and Opera...

Страница 14: ...r on the TFF system first before powering on Auxiliary Components 7 Connect TFF flowpath to TFF system 8 Set low and high pressure alarms and interlocks as required by the process conditions 9 Input Concentration Factor Diafiltration Volume CF DV set points into the TFF system s Pump Mode settings to start application Auxiliary Components Required for Pump Modes Pump Mode Feed Scale Permeate Scale...

Страница 15: ... a full setup with all Auxiliary Components 2 Auxiliary Pumps a Connect up to two auxiliary pumps to the Auxiliary Component Cable s Auxiliary Pump 1 and or Auxiliary Pump 2 ports b Connect auxiliary pump power cable s 3 Auxiliary Scales a Connect up to two scales to the Auxiliary Component Cable s Feed Scale and or Permeate Scale ports Feed Scale Permeate Pump Auxiliary Pump 2 Permeate Scale Diaf...

Страница 16: ...y Scales a Connect scale to the Auxiliary Component Cable s Permeate Scale 7 4 C D and C D C Mode Setup NOTE Permeate Pump is optional and shown in the diagram as an example for applications that require permeate control 1 Auxiliary Pumps Feed Reservoir KMPi Pump Permeate Reservoir Diafiltration Buffer Reservoir Module Diafiltration Pump Auxiliary Pump 1 Feed Scale Permeate Reservoir Permeate Pump...

Страница 17: ...rst auxiliary pump to the Auxiliary Component Cable s Auxiliary Pump 1 port and second auxiliary pump to the Auxiliary Component Cable s Auxiliary Pump 2 port b Connect auxiliary power cables c Auxiliary Pump 1 will function as Diafiltration Pump 1 and the Auxiliary Pump 2 will function as Diafiltration Pump 2 2 Auxiliary Scales a Connect first scale to the Auxiliary Component Cable s Feed Scale p...

Страница 18: ...d second auxiliary pump to the Auxiliary Component Cable s Auxiliary Pump 2 port b Connect auxiliary pump power cables c The Auxiliary Pump 1 will function as Constant Feed Pump 2 Auxiliary Scales a Connect first scale to the Auxiliary Component Cable s Feed Scale port and second scale to the Auxiliary Component Cable s Permeate Scale port Extra Feed Reservoir Module Feed Reservoir Permeate Reserv...

Страница 19: ...ump power cables c Auxiliary Pump 1 will function as Constant Feed Pump and Auxiliary Pump 2 will function as Diafiltration Pump 2 Auxiliary Scales a Connect first scale to the Auxiliary Component Cable s Feed Scale port and second scale to the Auxiliary Component Cable s Permeate Scale port KMPi Pump Diafiltration Pump Auxiliary Pump 2 Feed Scale Diafiltration Buffer Reservoir Permeate Scale Cons...

Страница 20: ...mponent Cable s Permeate Scale port 7 9 System Setup Using KF Comm Pump Control Window 7 9 1 KF Comm User Interface The Pump Control Interface can be used to interact with all the features of the system This screen will appear when the KF Comm software is opened in Excel When needed the functions and setpoints are accessible on the main pump display For full explanation of Pump Control window plea...

Страница 21: ...isabled when the valve is not pinching Only available to click when valves are pinching If Safe is checked Edit buttons and Tare buttons will be disabled Reference Calculator can still be opened Arrow slides the Live Values and Settings window shut Switches to Overview view Engaging the numeric valve input box brings up a floating keypad mini menu that can optionally be used to input the numbers i...

Страница 22: ...et and display alarms and stops Safe Checkboxes Checking the safe checkbox disables changing values Reference Calculator The Reference Calculator allows the user to run calculations for Pump Modes to estimate the volumes of solution that will be moved through the flowpath from the beginning to end of a Pump Mode application Switches to Set Points view While Function runs the process bar fills up t...

Страница 23: ...e application in Overview view View can be changed by clicking on Modify Set Points Overview button Progress Bar displays starting value on left hand side and target set point on right hand side The Progress Bar will fill as the Pump Mode runs The Safety Alarm bars will display different bands of color depending on what alarms have been set for an auxiliary component Process Parameters segment can...

Страница 24: ...scosity samples Select from 1 10 reduction in pump speed at either min or sec Control Shear If turned on the system will maintain recirculation pump speed based on retentate shear rate flow rate The Flowrate display in the Main Pump section will then be based on retentate not feed flow 2 Aux Pump Aux Pump 1 Aux Pump 2 Select the tubing size and the type of pump There are three types of possible Au...

Страница 25: ...0 3 3 Tubing Size Select the tubing size that goes through the backpressure valve Control Select the pressure control location Feed Permeate Retentate or TMP Starting Position Select the starting position for automated control from following options 1 Open no pinching of tubing 2 Half 50 closed based on selected tubing size in valve 3 Custom pinches the tubing based on the Custom Start Position va...

Страница 26: ...etpoint for the various warnings and interlocks Please refer to Section 7 12 for further definitions NOTE When the Overview option is selected for the bottom portion of the Pump Control window the alarm values will have thin lines indicating warning level and thicker lines to signal stop Find Comm Select to automatically find the USB Com port assigned to the TFF system About KF Comm Version inform...

Страница 27: ...n loop is full of liquid Permeate Status Select Empty if permeate side of the filter and permeate tubing is empty upon start of automated process Select Full if permeate side of the filter and permeate tubing is full of liquid Feed Hold up measure the amount of liquid in the feed holdup and enter the amount Volume can be measure by starting with an empty flowpath closing the permeate line and meas...

Страница 28: ... settings for concentration and diafiltration can be selected to be either by weight CF and DV or with the KONDUiT accessory to be UV or Conductivity Below are explanations and example applications for different control strategies Diafiltration 1 Set Point 1 DV Diafiltration Volumes a End point control when permeate scale reaches target DV based on weight of feed scale and holdup loop b Example Ap...

Страница 29: ...latex particle with free IgG measurement on permeate offline confirmation needed for verification Concentration 1 Set Point 1 CF Concentration Factor a End point control when retentate and permeate scale reaches target CF based on weight of feed scale and holdup loop b Example Application concentration of virus or protein 2 UV a Semi quantitative end point control when UV reaches target AU Applica...

Страница 30: ...mple Application concentration of oligo Here is an example of Hardware Setup once a system is connected NOTE Green highlighted boxes are currently selected 7 10 Using Pump Interface The system settings accessed through the Pump Control Window mentioned in Section 7 9 above can also be accessed directly on the pump console The TFF system s easy to use keypad allows the user to navigate through the ...

Страница 31: ...cer has been plugged Into a port the empty pressure transducer port will display dashes as readings When the TFF system is turned on and a pressure transducer has been plugged into a port the occupied pressure transducer port will display a value When the TFF system is turned on a pressure transducer is connected and then the pressure transducer is disconnected or becomes faulty the port will disp...

Страница 32: ...se capture start pause and change the flow rate of Auxiliary Pumps see Section 7 10 3 Remote Control of Auxiliary Pumps and Valves for details j Pump Mode Status Timeout Display When Auxiliary Scales or KF KONDUiT are connected and powered on the user will be able to view the Pump Mode Status Timeout Display The Timeout Display will show weight and Conductivity Temperature UV when using KONDUiT in...

Страница 33: ...stead of being controlled by the TFF system NOTE While Auxiliary Pump is in Internal mode the Flow Direction of the Auxiliary Pump can be changed However once switched back to External Mode and recaptured the Auxiliary Pump will only pump in a Clockwise Flow Direction Auxiliary Pump Flow Direction is always Clockwise when part of the TFF system 4 A Released Pump can also be re captured a First sto...

Страница 34: ...ter to exit the Valve mini menu 3 Press the right arrow on the highlighted Control icon to start pinching If on Manual mode hold down either the left or right arrow afterwards to manually adjust the pinch distance Pressing holding the left arrow will decrease pinch distance while pressing holding the right arrow will increase pinch distance When satisfied with manually set pinch distance user may ...

Страница 35: ...accurate fluid dispensing tubing can not be calibrated if pump is set to display RPM f Aux Pumps TFF system s Aux Pump settings must be configured prior to using the Auxiliary Pumps to ensure the TFF system sends the correct commands to each pump g Hardware Setup Time date unit of measure and some Pump Mode options may be set from the Hardware Setup menu described in more detail in the next sectio...

Страница 36: ...Valve is unable to control pressure then main drive will slow down gradually to control pressure increases especially when concentrating viscous solutions p Ramp Seconds Determines rate at which TFF system will increase main pump flow rate linearly over desired amount of time q Minus Modes Enables TFF system to use weight set points instead of CF DV setpoints Once turned on KF Comm needs to be clo...

Страница 37: ...t Pressure Calibrate from Settings Menu 7 Select source of pressure in this case Feed then adjust Set pressure reading to reflect that of calibrated source 8 Save changes by selecting Cal 9 Repeat steps 3 to 8 with the Retentate and Permeate ports 7 12 Alarms and Stops The TFF system has a number of safety pressure alarms and stops that may be set in the Alarm Settings menu a Pf Hi Stop When Feed ...

Страница 38: ...l shut down m Feed Lo Alarm When feed scale reading is Feed Lo Alarm value the system will alarm but continue running n Feed Lo Stop When feed scale reading is Feed Lo Stop value the system will shut down o Silent Alarm Set to On to silence alarms set to Off to play alarm sound 1 To enable an alarm highlight the alarm value and press Enter 2 Use the directional keys to set the desired alarm value ...

Страница 39: ...e the Pump Control layout in section 7 9 1 KF COMM Pump Control User Interface for further information using the Pump Control Interface on the PC 8 1 1 Overview The TFF system alternates through different functions based on user defined set points suitable for the user s application these combinations of functions are Pump Modes All Pump Modes are composed of different sequences of Concentration a...

Страница 40: ...e flushed with 16L of water To automate flushing the module on the TFF system while only using a Permeate Scale the user can simply start with 32 kg of water in the Feed Reservoir input the value into the TFF system as 32L of Feed Hold up and set Feed Status Empty Then the user must set the Conc Factor setpoint to 2X Once 16 kg of water has been moved from the Feed scale to the Permeate Scale C Mo...

Страница 41: ...On the Feed Reservoir with four ports set up the ports as described below to create a vacuum that will draw excess feed into the Feed Reservoir from the Extra Feed Reservoir 1 Feed Line to Filter Connect tubing from this line through pump head to filter s feed port 2 Retentate Line from Filter Connect tubing from filter retentate port to this line 3 Buffer Connect through Auxiliary Pump 1 to Diafi...

Страница 42: ...Feed Reservoir are depleted the vacuum will pull air After the Concentration set point is reached Conc Factor 1 the TFF system will then switch to D mode until the Diafiltration set point Dia Volumes 1 is reached The TFF system will be able to properly calculate the Concentration Factor because it will know that a Fed Batch Concentration is taking place when the Permeate Scale increases while the ...

Страница 43: ...r Diafiltration Starting Weight 20 kg Conc Factor 1 10X Perm Wt After C1 18 kg Feed Wt After C1 2 kg Diaf Volumes 1 7 Diaf Wt 1 14 kg Perm Wt After D1 32 kg Conc Factor 2 20X Feed Wt After C2 1 kg Feed Scale Permeate Scale Feed Reservoir KMPi Pump Permeate Reservoir Diafiltration Buffer Reservoir Module Diafiltration Pump Auxiliary Pump 1 Feed Scale Permeate Pump Auxiliary Pump 2 Permeate Scale ...

Страница 44: ...voir and have one line go through Auxiliary Pump 1 and the other line go through the Auxiliary Pump 2 Starting Weight 20 kg Conc Factor 1 10X Perm Wt After C1 18 kg Feed Wt After C1 2 kg Diaf Volumes 1 7 Diaf Wt 1 14 kg Perm Wt After D1 32 kg Diaf Volumes 2 8 Diaf Wt 2 16 kg Perm Wt After D2 48 kg Conc Factor 2 20X Feed Wt After C2 1 kg Feed Scale Permeate Scale Diafiltration Buffer Reservoir 1 Mo...

Страница 45: ...he Extra Feed Reservoir completely or stop the Aux Pump 1 at a Concentration Factor setpoint NOTE If the depletion of the Extra Feed Reservoir is selected CF might exceed setpoint NOTE Aux Pump 1 will stop running once the feed scale detects no additional sample added Extra Feed 20L Starting Weight 20 kg Conc Factor 1 10X Perm Wt After C1 36 kg Feed Wt After C1 4 kg Feed Reservoir Module Permeate ...

Страница 46: ...tion of the Extra Feed Reservoir is selected CF might exceed setpoint NOTE Aux Pump 1 will stop running once the feed scale detects no additional sample added Extra Feed 10L Starting Weight 20 kg Conc Factor 1 10X Perm Wt After C1 27 kg Feed Wt After C1 3 kg Diaf Volumes 1 7 Diaf Wt 1 21 kg Perm Wt After D1 48 kg Conc Factor 2 20X Feed Wt After C2 1 5 kg Feed Scale Permeate Scale Feed Scale Diafil...

Страница 47: ...nfiguration The Data Collection and calculations on KF Comm will reflect which of the two functions is running until either the Permeate Weight Stop set point is reached or the user presses Stop on the Pump or Pump Control Control Mode can only be accessed properly using KF Comm on a computer KMPi Pump Permeate Reservoir Diafiltration Buffer Reservoir Feed Scale Vacuum generated in sealed vessel P...

Страница 48: ...F Comm Pump Control see Section 7 10 4 for Pump Control 1 Set the flow rate of the TFF system in the Main Screen 2 Select a Pump Mode suitable for application see Section 12 4 for Sample Applications 3 Input set points required for the Pump Mode 4 Press the Start Pause soft button on the TFF system to start Pump Mode or the green START button on top line of Pump Control window When each set point ...

Страница 49: ...d Operation 9 1 Introduction The Easy Load and High Performance Pump Heads are designed to be used with the TFF systems as a simple peristaltic pump system The Pump Heads accept different tubing sizes for a wide range of flow rates and the unique designs and automatic tubing retention allow for quick tubing changes 9 2 Specifications KR2i Pump Heads Part Number Roller and Bearing Material Number o...

Страница 50: ...g materials Sealed stainless steel Rotor materials Stainless steel Chemical resistance Most substances except strong acids or alkalis organic solvents or hydrocarbons Temperature Operating 32 F to 104 F 0 C to 40 C Temperature Storage 49 F to 149 F 45 C to 65 C Humidity 10 to 90 non condensing Altitude 2000 m or less Dimensions W x H x D Excluding shaft and cosmetic cover Operating 3 45 x 4 75 x 3...

Страница 51: ...0 to 40 C 32 to 104 F As tested with NORPRENE PHARMED and TYGON tubing Values will be less with silicone and C FLEX Use in this temperature range for continuous duty operation with no decrease in performance or life Pump heads will work outside this range with some possible reductions in performance or product life Part Number Roller and Bearing Material Number of Rollers MasterFlex I P Tubing ACM...

Страница 52: ...stalling or removing the pump head from the drive 1 If mounting plate is not attached to the pump drive attach it using the provided four Phillips head screws see Figure 1 2 Orient the pump head with its back facing the drive and insert the tang on the pump head shaft into the shaft s slot on the drive Align the bayonet features on the back of the pump head with the bayonet tabs on the front of th...

Страница 53: ... that it is firmly centered against the rollers While holding the tubing ends move the actuator lever back to the far clockwise right position as shown in Figure 5 The pump head will automatically grip the tubing Approximately 5 pounds of force must be applied to the actuator lever to fully close the pump head and place the lever in its locked position far right position or to fully open the pump ...

Страница 54: ... pump head Grasp the cover by the notches and pull it off see Figure 8 Align the second pump head to the first as if the first pump head were the drive and continue to follow pump head mounting instructions see Figures 9 and 10 NOTE The tubing on the inner pump head s can be changed without removing the outer pump head s from the drive Figure 7 Tubing path through pump head during loading Occlusio...

Страница 55: ...r is required to complete installation a Check that tang boot has plastic dampening to prevent damage to the pump shaft b Raise occlusion bed to expose mounting holes for top two short screws of Easy Load Pump Head c Aim back of Pump Head towards mounting plate to align pump shaft tang and five screw holes for Easy Load Pump Head or four screw holes for High Performance Pump Head NOTE Inspect the ...

Страница 56: ...to the right to close the occlusion bed For High Performance Pump Head press occlusion bed against the tubing snap the latch close close cover and pull tubing snugly around rotor assembly Finish by tightening tubing retainer by rotating tubing retainer knob clockwise to prevent tubing from moving and run the drive for at least one revolution to break in tubing Unlatch the bed from Slot 1 and then ...

Страница 57: ...fluid begins to flow KMPi Multi Channel NOTE Do not overtighten screws or wing nuts To mount two KMPi Easy Load Pump Heads use the additional mounting hardware that comes with the purchase of a second set of KMPi Easy Load Pump Heads The High Performance Pump Head is incompatible with Multi Channel Mounting a Aim the three mounting holes and pump shaft tang on the back of the second Easy Load Pump...

Страница 58: ...iewing all of the SAW literature prior to running the SAW scale TFF systems are also compatible with other scales including some Ohaus Mettler and other manufacturers Please contact Repligen for more information on scale compatibility 10 1 1 Installation 1 Carefully unpack scale from shipping carton 2 Place scale on a level surface and adjust the level legs so that all four legs are touching the s...

Страница 59: ...2 1 Installation Up to two auxiliary pumps can be connected to the TFF system s Auxiliary Component Octopus Cable After connecting auxiliary pump to TFF system and the TFF system has been powered on the Auxiliary Pumps can then be powered on and used in its internal or external modes depending on the TFF process To have the TFF system control the auxiliary pump ensure that the auxiliary pump is in...

Страница 60: ...red Auxiliary Pump cord on KMPi s Auxiliary Component Octopus Cable 3 Power on I P Digital Peristaltic pump 4 Select ENGLISH if prompted to select language and press ENTER key 5 Use up arrow to select CONTINUOUS MODE if prompted and then press ENTER key 6 If pump shows after starting up Figure 2 it is already in Remote Control Mode and is ready to use Skip ahead to Configuration of KMPi steps If p...

Страница 61: ...the PC or Section 7 10 3 for setting the Aux Pump settings on the main pump console 10 3 Automatic Backpressure Valve The KrosFlo Automatic Backpressure Valve controls a wide variety of pressure set points during tangential flow filtration processes when used in conjunction with the TFF System The valve is designed to pinch flexible tubing to maintain the user set pressure Up to one ABV may be con...

Страница 62: ...ubing Size Used to determine pinch distance d Control Set whether Valve is controlling Feed Permeate Retentate or TMP pressure e Start Position The initial Start position before adjusting pinch distance Open is no pinching Half is 50 closed based off of tubing size Close is 100 closed based off of tubing size Custom is a custom position set by user Manual Mode Menu a Mode Can be set to Manual or A...

Страница 63: ...ody Optional Single use UV Flowpath Components UV in line flowpath sensors made of Polysulfone and in assorted hosebarb sizes 10 4 2 Installation Basic Setup NOTE Prior to assembling KONDUiT ensure that TFF System has been properly set up see Section 7 of KR2i KMPi TFF Systems Product Information and Operating Instructions for TFF System setup details 1 With TFF System powered on connect KONDUiT C...

Страница 64: ...ng Absorbance For UV Dialfiltration automated control sequences the sensors are usually placed in the permeate line and the endpoint should be lower than the starting Absorbance as the protein or other sample is washed through Place the tubing through the plunger mechanism by lifting up on the body of the valve and fitting the tubing between the metal bar and the white plastic plunger The body of ...

Страница 65: ...lish connection with TFF System 2 Launch KF Comm Data Collection workbook for TFF System a COMM port selection error may appear this is normal as the default COMM port that KF Comm will use is usually not the actual COMM port the TFF System was read as by the computer To connect KF Comm to COMM port a Click on KF Comm Config gear icon b Click on Find Comm to have KF Comm consecutively test all ava...

Страница 66: ...Control Strategy for description of how to setup and use automated setpoints with Conductivity and UV measurements for Concentration and Diafiltration steps 7 Press Start to start the application a After pressing Start the user may then press Stop to stop the application or Pause to suspend the application i After pressing Stop the user can press Start again to start a new application ii After pre...

Страница 67: ...ltration set Dia Set Point option to DV s NOTE KF Comm will still collect data from channels that were not selected to be used to end Diafiltration these data points will be for reference only and will still trigger Alarms b In any Pump Mode that has at least one Diafiltration step user can now input a KF KONDUiT set point to end Diafiltration step s in Pump Mode menu 6 Press Play Pause softkey to...

Страница 68: ...d warranties or merchantability and fitness for a particular purpose are disclaimed In no event shall the copyright owner or contributors be liable for any direct indirect incidental special exemplary or consequential damages including but not limited to procurement of substitute goods or services loss of use data or profits or business interruption however caused and on any theory of liability wh...

Страница 69: ...trum KF Comm Installer will check the system settings press Proceed to continue 3 Check Install KF Comm software and workbooks and Install USB drivers for pump drive connection recommended If user is also updating firmware please select Update KMPi Pump and or Update KR2i Pump depending on which TFF system is being used 4 Proceed through the default installation steps When at KF Comm Data Collecti...

Страница 70: ...o and wait 15 30 minutes for TFF system to complete firmware update TFF system LCD screen will go dark until firmware update is completed 12 If this is not the first time user has updated the firmware then user may be prompted to either save or discard update log from most recent update If this is the first time the user has updated the firmware or the user has decided to either save or discard th...

Страница 71: ...ning the proper TFF system template ensure that user has Administrator or similar access to both the computer and Excel 2 To open the TFF system template the user may a Open the Spectrum Labs folder with shortcuts on the desktop then select the KR2i or KMPi Data Collection shortcut link b Click on the Start Menu and type in KR2i Data Collection or KMPi Data Collection then click on the retrieved f...

Страница 72: ... the Pump Control and data can now be collected 11 5 Data Collection Tools Data Collection Tools may be found in the Add ins tab on the Excel ribbon Different versions of Excel may also display graphical icons to represent the tools a End Control Used to end data collection Certain functions in Excel need to be disabled for the data in the TFF Trial Template to be accessible Clicking this will sto...

Страница 73: ...cense Calls up a dialog with licensing information h About KF Comm Calls up a dialog with basic KF Comm information 11 6 Worksheets Overview a Integrity Test Collect data with this worksheet during integrity testing to ensure membrane integrity b Module Characteristics Collect data with this worksheet when analyzing initial water flux c Header Contains information that is used to populate the Head...

Страница 74: ...affecting module performance Caking is influenced by fluid variables degree of solvation concentration and nature of the solids and solutes fluid temperature along with operating variables such as the solution s velocity along the membrane and TMP Controlling this phenomenon by ensuring adequate fluid velocity at the liquid membrane wall interface will maximize flux solute passage and optimize pro...

Страница 75: ...mber of times Troubleshooting Sympton Diagnosis Remedy 1 Pressure Sensor readings are wrong either 9 9 psi or 35psi when no pressure on them Pressure sensor octopus cable not functioning or pressure sensor broken Replace Octopus Cable and or pressure sensor 2 Scale not reading properly Refer to manufacturer s scale manual Refer to manufacturer s scale manual 3 Pump not working properly Refer to pu...

Страница 76: ...5TC C1N Pressure Sensor PS 1 TC Non irr ACPM 10TC 01N Pressure Sensor PS 1 TC Irradiated ACPM 10TC 01S Pressure Sensor PS 1 TC Calibrated Non Irr ACPM 10TC C1N Auxiliary Part KONDUiT Part Number KONDUiT Base Unit ACCD B Conductivity Sensor PS Non irr HB ACCS 12HB Conductivity Sensor PS Non irr HB ACCS 14HB UV 260nm Photometer ACCD U260 UV 280nm Photometer ACCD U280 UV Flow Cell 1 0CM PL PS Non irr...

Страница 77: ...4 Conductivity 11 28 29 32 40 49 62 63 64 66 75 76 Ctrl Mode 14 20 47 48 Danger 7 Diafiltration 14 17 19 22 27 28 29 34 36 39 40 41 42 43 44 46 47 48 64 65 66 67 75 Hardware Setup 26 27 30 31 35 40 48 65 66 67 KF Comm 6 12 13 20 23 26 35 36 39 47 48 64 65 67 68 69 71 72 73 KONDUiT 6 10 11 28 32 36 37 40 48 49 62 63 64 65 66 67 68 76 KR1 24 59 76 KRJr 76 M Mode 14 48 Octopus Cable 12 13 37 59 60 61...

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