RainDance RainDrop Sense, Руководство оператора

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RainDrop Sense Operator’s Manual
LCN 50-04343 Rev. D 7-11
I tried to fill the oil reservoir
before the oil light came on. It is possible to overfill the
oil reservoir and to spill oil
into internal areas of the
instrument. You may not
notice it, even when you
have done this. For this reason, do not
refill the oil reservoir until
the oil light comes on.
There is dirt, debris, or oil
residue on the instrument. The instrument has not
been properly cleaned. Clean the instrument
according to the
instructions in
Cleaning the
RainDrop Sense on page
7-2
.
The sample has evaporated.
The sample volume in one or
more wells is reduced or totally
gone. The droplet count is
lower than expected.
The tube strip or the tube strip
cap was deformed during
thermal cycling and resulted in
a leak into the PCR tube strip
nest of the Sense.
The tube strip cap was not
seated properly or the tube
strip was not covered
within 30 minutes of
Source run completion. Ensure that the tube strip
cap is seated properly. It
should be even along the
entire length of the tube
strip. An extra cap is
supplied in each kit; if you
find one that does not fit
properly, discard it and use
another.
Do not reuse tube strip
caps. Caps are pierced by
the Sense instrument as
part of the process.
Pierced tube strip caps
allow evaporation and
introduce the potential for
cross contamination.
Clean the PCR tube strip
nest on the Sense
instrument. Use the
recommended cleaning
protocol. See
Cleaning the
RainDrop Sense on page
7-2
.
One lane or the entire run has
failed. There may be dirt, debris,
scratches, or smudges on
the imaging region of
interest on the Sense Chip. Handle chip as
recommended in
Handling
the Sense Chip on page 2-
6
.
Keep the chip dock clean
as described in
Cleaning
the RainDrop Sense on
page 7-2
.
Table 7-1: Troubleshooting the RainDrop Sense Instrument