Page
20
of
30
8.
TECHNIQUES FOR OPTIMIZING RESULTS
Probe size vs. Sample volume
Selecting the proper size probe is a critical factor when sonicating a sample. The sample volume
to be processed must correlate with the tip diameter. Each probe has a recommended sample
volume range. This range may overlap with other probes. For example, the ½” probe can
process approximately 20-250ml. Depending on the type of sample you may be able to process
a little less than 20ml or more than 250ml. Depending on the vessel size and shape, the ½”
probe may have difficulty fitting inside a 20ml volume and a ¼” microtip may be a better option.
Many factors must be considered when selecting the appropriate probe for your application.
Small volumes require a small tip to fit inside the sample tube. Small tips (or Microtips) are
recommended for processing samples inside small, thin vessels and never samples larger than
50ml. Microtips are high intensity and made for short processing times. Using a microtip for long
time periods will generate a considerable amount of heat. Microtips should be used in pulse mode
to reduce heat buildup.
Larger volumes require a larger probe for effective processing. For example, a 1” probe will
process 1 liter much faster than a ¾” probe. Using the proper size probe will not only reduce the
processing time but increase the lifespan of the probe. The addition of a stir bar can greatly aid
processing of large samples. A probe should not be used to process a volume larger than
indicated on the chart unless the application is reviewed and approved by a Qsonica
representative.
While there is no absolute sample volume range for any probe/horn, below is a general guideline
to follow. Processing volumes are application specific. Contact us for more details.
Tip Diameter
Processing Volume Range
1/16" (2mm)
0.2ml - 5ml
1/8" (3mm)
1ml - 15ml
1/4" (6mm)
10ml - 50ml
1/2" (12mm)
20ml - 250ml
3/4" (19mm)
50ml - 500ml
1" (25mm)
100ml - 1,000ml
1" with booster
500ml – 2,000ml
Vessel shape and size
A narrow vessel is preferable to a wide vessel. The ultrasonic energy is generated from the tip
and is directed downward. As a sample is processed the liquid is pushed down and away in all
directions. If the vessel is too wide, it will not mix effectively and some sample will remain
untreated at the periphery. The probe should never touch the sides or bottom of the vessel.
Содержание Sonicator Q500
Страница 2: ...Page 2 of 30 ...
