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102

12.4

Measuring Principle

The new

REDFLASH technology

is based on the unique oxygen-

sensitive

REDFLASH indicator

showing excellent brightness. The

measuring principle is based on the quenching of the

REDFLASH

indicator

luminescence caused by collision between oxygen

molecules and the

REDFLASH indicator

immobilized on the sensor

tip or surface. The

REDFLASH indicators

are excitable with red

light (more precisely: orange-red at a wavelength of 610-630 nm)
and show an oxygen-dependent luminescence in the near infrared
(NIR, 760-790 nm).

The

REDFLASH technology

impresses by its high precision, high

reliability,  low  power  consumption,  low  cross-sensitivity,  and  fast
response  times.  The  red  light  excitation  significantly  reduces
interferences  caused  by  autofluorescence  and  reduces  stress  in
biological  systems.  The

REDFLASH indicators

show much higher

Содержание FireStingO2

Страница 1: ...1 FireStingO2 FIBER OPTIC OXYGEN METER USER MANUAL...

Страница 2: ...r Software version 3 1 The FireStingO2 is manufactured by PyroScience GmbH Hubertusstr 35 52064 Aachen Germany Phone 49 0 241 4004 555 Fax 49 0 241 4004 558 Email info pyro science com Internet www py...

Страница 3: ...RS 25 6 THE SOFTWARE PYRO OXYGEN LOGGER 27 6 1 MAIN WINDOW 27 6 2 SETTINGS 35 6 2 1 Basic Settings 37 6 2 2 Advanced Settings 38 6 2 3 Conditions in the Sample 39 6 2 4 Temperature 41 6 2 5 Options 43...

Страница 4: ...2 THE 0 STANDARD 78 8 2 1 Water Mixed with a Strong Reductant 78 8 2 2 Water Flushed with Nitrogen Gas 79 8 2 3 Nitrogen Gas 79 9 CALIBRATION OF CONTACTLESS SENSORS 80 9 1 CALIBRATION PROCEDURE 80 9...

Страница 5: ...5 12 4 MEASURING PRINCIPLE 102 12 5 OPERATING SEVERAL FIRESTINGO2 IN PARALLEL 104 12 6 DEFINITION OF OXYGEN UNITS 105 12 7 TABLE OF OXYGEN SOLUBILITY 107 12 8 EXPLANATION OF THE SENSOR CODE 109...

Страница 6: ...piration vials and working with full range and trace oxygen sensors The FireStingO2 has integrated atmospheric pressure and humidity sensors for a precise and easy sensor calibration but also for auto...

Страница 7: ...s well as customized OEM solutions More information concerning our products can be found at www pyro science com or contact us under info pyro science com We would be pleased to serve you concerning a...

Страница 8: ...nsors should be kept and stored outside the reach of children in a secure place under dry and clean conditions at room temperature avoiding moisture dust corrosive conditions and heating of the instru...

Страница 9: ...data processing and data publication is on the user s authority When used in the field the environmental conditions like high humidity dust exposure to direct solar radiation may cause damage or inte...

Страница 10: ...f the protective cap Prevent mechanical stress to the fragile sensing tip Avoid strong bending of the fiber cable Prevent injuries with needle type sensors Calibration and application of the sensors i...

Страница 11: ...etails The FireStingO2 is a high precision compact USB powered fiber optic oxygen meter with 1 2 or 4 channels for measurements in the laboratory Additionally one temperature sensor can be connected f...

Страница 12: ...n the left side panel provides the energy supply and the data exchange with the PC Right hand side of it a connector X1 for power and digital interface 7 pins and a connector X2 for analog output 5 pi...

Страница 13: ...ownload the installer package for the newest version of the Pyro Oxygen Logger software from the PyroScience homepage www pyro science com downloads html unzip and start the installer and follow the i...

Страница 14: ...ingO2 meter a metal housing H fixed or retractable and a syringe needle N 40 mm standard length including the fiber with a fragile sensing tip and protected with a protective cap PC On each sensor a s...

Страница 15: ...with 0 5 mm and 1 1 mm tip diameter C The retractable sensors are available as microsensors OXR50 A and as minisensors OXR230 OXR430 B The retractable sensor tip allows sensor insertion through septa...

Страница 16: ...3 In position 1 the sensor tip is flush with the needle tip whereby in positions 2 or 3 the fragile sensor tip extends ca 6 mm or 12 mm out of the needle Please take care when handling the needle typ...

Страница 17: ...oid any contact to harsh surfaces The needle type oxygen sensors are also optionally available with optical isolation for measurements in samples with auto fluorescence With this optical isolation int...

Страница 18: ...ry stand loosen the clamping screw and carefully pull out the bare fiber sensor NOTE After removal of the protective plastic tube the fragile sensor tip is unprotected and sensitive to breakage CAUTIO...

Страница 19: ...B10 OI which is recommended for applications with strong external illumination like direct sun light The Robust Oxygen Probes can be used for long term measurements in gases and liquids The whole sens...

Страница 20: ...oScience offers a range of contactless oxygen sensors comprising sensor spots respiration vials and flow through cells The sensor spots are coated with the REDFLASH indicator with PET foil or glass as...

Страница 21: ...a 10x10x10 mm plastic cube item no SPADBAS needs to be fixed tightly or glued to the outer container wall at the spot position Then the sensor signal can be read out using the Optical Fiber item no S...

Страница 22: ...lowing online monitoring of the oxygen content in a gas or liquid sample pumped through the cell The flow through cells are offered as a small version item no OXFTC and as a large version item no OXFT...

Страница 23: ...ngs and the spot fiber can be fixed by clamping screws The elongated shape of the built in sensor allows oxygen measurements at different heights within the vial by simply sliding the adapter rings up...

Страница 24: ...ors of the FireStingO2 1 to 4 with a male fiber plug First remove the black caps from the plug of the sensor fiber Remove the red caps from the receptacles at the FireStingO2 the red caps should be pu...

Страница 25: ...sor spots this could require a re positioning of the optical fiber on the sensor spot and a subsequent new calibration If the signal intensity is getting too low as indicated by the horizontal indicat...

Страница 26: ...the sensor surface For all sensors and fibers put the black caps on the plug of the fiber to prevent that light is entering the fiber possibly causing photo bleaching of the REDFLASH indicator For sen...

Страница 27: ...ter start of the software Pyro Oxygen Logger the following main window is shown The four panels Channel 1 4 correspond to the channels 1 4 of the fiber optic oxygen sensors connected to the FireStingO...

Страница 28: ...see chapter 6 2 the sensor readings of each channel are displayed in its corresponding panel in a numeric display D and in a chart recorder C in the chosen oxygen unit UD The color and appearance of e...

Страница 29: ...units of degree Celsius C NaN denotes Not a Number when no temperature sensor is connected or if it is not activated in the Settings see chapter 6 2 4 A warning Bad Temperature appears below the chart...

Страница 30: ...X Scale Time can be changed by clicking on the respective selector or by double clicking directly onto the field and entering the values manually changing these parameters will automatically de activ...

Страница 31: ...alibrated the warning Not Calibrated is shown on the right hand side of the Calibrate button As long as the sensors are not calibrated the data are shown in units of raw value reflecting only qualitat...

Страница 32: ...g to switch between different laboratory setups with a single FireStingO2 This function might be also useful if different computers are used for the calibration and for the actual measurements You mi...

Страница 33: ...The different windows operate completely independent of each other and are assigned to exactly one FireStingO2 This allows measurements in different setups at the same time The flashing of the logo f...

Страница 34: ...th a sample interval 10 s data smoothing is done by a simple running average e g with Data Smoothing 5 always the last 5 sampled data points are averaged However for single data point measurements and...

Страница 35: ...w File The data saving is indicated by the light green arrow in the grey Log to File button and can be stopped by clicking this button again NOTE During data logging the buttons Settings and Calibrate...

Страница 36: ...of the main window and also in the data file Please take care that the sensor code attached to the sensor of a specific channel of the FireStingO2 is entered into the field Sensor Code in the same cha...

Страница 37: ...n entered in the field Sensor Code For contactless sensors sensor spots flow through cells respiration vials sensor type S W T and for robust probes sensor type X additionally the Fiber Length m of th...

Страница 38: ...in chapter 9 2 Finally the background compensation can be completely de activated by selecting No Background Compensation NOTE Generally it is advised to select Fiber Length m and to enter there the...

Страница 39: ...oxygen sensor Later re adjustments in the Advanced Settings might require also a recalibration of the sensor 6 2 3 Conditions in the Sample The next step is the determination of the Conditions in the...

Страница 40: ...with an external thermometer or with a temperature sensor from PyroScience connected to the FireStingO2 and has to be adjusted manually This Compensation Temperature either entered manually at consta...

Страница 41: ...levant if a concentration unit for oxygen was selected e g mg L or M L 6 2 4 Temperature An External Temperature Sensor connected to the FireStingO2 and the Internal Temperature Sensor inside the Fire...

Страница 42: ...e of the temperature measurement or b decreased in order to achieve higher sampling rates A Manual Offset of the External Temperature Sensor might be entered for possible recalibration of the temperat...

Страница 43: ...and saved into the data file If the Internal Pressure Sensor of the FireStingO2 was activated in one of the oxygen channels see 6 2 3 it is automatically activated in the Options panel It is possible...

Страница 44: ...ues 10000 might decrease the maximum sample rate The FireStingO2 offers four analog outputs 0 2 5V at the extension port which can be configured by pressing the Analog Output button For details refer...

Страница 45: ...ew graph The plot style of each channel in the chart can be changed by clicking on the color control By clicking on the small rectangular button of the color control it is possible to show or hide the...

Страница 46: ...elta phi dphi in dphi is the actual measured raw value which is used for the internal calculation of the oxygen concentration Oxygen M Oxygen partial pressure hPa Oxygen in air saturation air sat and...

Страница 47: ...displayed On the left side the Status and different warnings can be indicated by the software concerning the signal and reference intensity too low too high and the detection of the temperature senso...

Страница 48: ...sical full size USB connector firmware 2 30 then proceed with chapter 7 2 For many calibration modes the atmospheric pressure and the relative humidity of the ambient air are important parameters ensu...

Страница 49: ...the calibration mode for the corresponding channel can be selected Five standard modes can be chosen for the calibration Factory calibration for a quick rough calibration taking the 0 and the air cali...

Страница 50: ...to determine the air calibration value in air saturated water if the measurements will be performed in water samples aqueous liquids During the calibration of a sensor connected to a specific channel...

Страница 51: ...It is only possible if the correct Sensor Code has been entered in the Settings see chapter 6 2 If the calibration mode Factory Calibration is chosen ensure that the correct sensor code has been ente...

Страница 52: ...humidity from the internal pressure and humidity sensors it is important that both the FireStingO2 device and the connected oxygen sensor are exposed to identical environmental conditions If possible...

Страница 53: ...was selected ensure also stable temperature readings indicated at Temperature Sensor C Note that the button Set Air will be highlighted as soon as the oxygen readings are within the expected range fo...

Страница 54: ...The preparation of appropriate air calibration standards is explained in chapter 8 The 0 calibration value is taken from the Sensor Code Note that this calibration mode will automatically read the atm...

Страница 55: ...tant calibration conditions If the external temperature sensor is selected ensure that the temperature sensor is placed close to the oxygen sensor Wait now until the oxygen sensor reading is stable by...

Страница 56: ...calibration value are determined from a manual calibration Ambient air is used for determining the air calibration value and a specially prepared 0 calibration standard for determining the 0 calibrat...

Страница 57: ...be highlighted as soon as the oxygen readings are within the expected range for the connected sensor type If all readings have reached their steady state click on Set Air and the actual oxygen sensor...

Страница 58: ...type sensors e g OXR50 OXR230 OXR430 it is important that the sensor tip is extended to position 2 or 3 see chapter 5 1 when the calibration value is taken 7 1 5 Calibration Mode 2 Point in Water or H...

Страница 59: ...on Then the temperature of the air calibration standard needs to be determined Either a Fixed Temperature is adjusted manually or the temperature is read from the external temperature sensor by select...

Страница 60: ...mperature is adjusted manually or the temperature is read from the external temperature sensor by selecting External Temperature Sensor Wait until the oxygen sensor reading is stable by observing the...

Страница 61: ...e allows the user to combine freely all possible calibration types for the air calibration and the 0 calibration The air calibration type can be selected by clicking on the Air Calibration Selector Th...

Страница 62: ...following The oxygen level in the calibration standard can be freely chosen in Oxygen O2 If the air calibration standard is based on ambient air or air saturated water then this value should be kept...

Страница 63: ...n be selected alternatively This option will read the ambient atmospheric pressure automatically from the integrated pressure sensor in the FireStingO2 The temperature of the calibration standard need...

Страница 64: ...ry refer also to chapter 0 If 0 calibration was selected follow the instructions given in chapter 7 1 4 Click on Finish for reverting to the main window NOTE If using retractable needle type sensors e...

Страница 65: ...ements 1 Point taking the 0 value from the sensor code and the air calibration value from a manual calibration for precise measurements around 21 O2 2 Point taking the 0 and the air calibration value...

Страница 66: ...y calibration is completed thereby returning to the main window of the corresponding channel 7 2 2 Calibration Mode 1 Point 1 Generation FireSting NOTE The calibration mode 1 Point is only possible if...

Страница 67: ...sted Either a Fixed Temperature is adjusted manually or the temperature is read from the external temperature sensor placed close to the oxygen sensor by selecting External Temperature Sensor Also the...

Страница 68: ...e needle type sensors e g OXR50 OXR230 OXR430 it is important that the sensor tip is extended to position 2 or 3 see chapter 5 1 when the calibration value is taken Wait for steady state until the sen...

Страница 69: ...ation value are determined in specially prepared calibration standards The preparation of appropriate 0 and air calibration standards is explained in chapter 8 The air calibration standard and the cal...

Страница 70: ...ype sensors e g OXR50 OXR230 OXR430 it is important that the sensor tip is extended to position 2 or 3 see chapter 5 1 when the calibration value is taken Subsequently insert the oxygen sensor and the...

Страница 71: ...ion The air calibration type can be selected by clicking on the Air Calibration Selector The 0 calibration type can be selected by clicking on the 0 Calibration Selector The following air calibration...

Страница 72: ...artial Volume of Oxygen 20 95 O2 can be defined as well as the calibration conditions Temperature Air Pressure Humidity as described for the Custom Calibration see chapter 7 1 6 The Temperature C need...

Страница 73: ...mt Tofs and tt are needed for the internal calculation of the oxygen concentration data These parameters are specific constants for the REDFLASH indicators and are automatically adjusted for the sele...

Страница 74: ...closed rooms occupied by many people or e g candles combustion engines consuming the oxygen So if in doubt ensure a good ventilation of the room with fresh air e g by opening a window for some minutes...

Страница 75: ...and Air Saturated Water see section 8 1 3 Another parameter even more important for the air calibration standard is the atmospheric pressure The principle parameter measured by oxygen sensors is not t...

Страница 76: ...he internal or external temperature sensor will measure these parameters automatically for most calibration types see also chapter 7 8 1 1 Ambient Air If ambient air is used as the air calibration sta...

Страница 77: ...gen sensor and a temperature sensor Stream for about 10 minutes air through the water with an air stone connected to an air pump available as commercial equipment for fish aquaria Alternatively if no...

Страница 78: ...ter e g sea water for this because the high salinity of the water might prevent a proper dissolution of the reductant Stir the solution until the salt is completely dissolved and let the solution stan...

Страница 79: ...ination of the 0 calibration standard 8 2 3 Nitrogen Gas Flush 100 nitrogen gas through a glass flask e g Duran flask with a lid prepared with holes for inserting the oxygen sensor and a temperature s...

Страница 80: ...tical to the relative humidity outside the setup experienced by the FireStingO2 However for typical applications around room temperature and below a falsely determined relative humidity gives a maximu...

Страница 81: ...gger software So the user usually does not notice the background compensation at all For normal applications this should be the preferred procedure But for precision applications and especially for lo...

Страница 82: ...on Take Manual Values If you enter manually zero for Background no background compensation is performed After the background compensation is finished the window closes and the program proceeds with th...

Страница 83: ...is only specified for long term immersion of the 100 mm long probe tip into aquatic samples However the complete TDIP15 including the cable is splash proof and withstands easily short term submersion...

Страница 84: ...ure readings are also saved into the data file For measurements in a gas sample either the External Temperature Sensor or the Internal Temperature Sensor can be chosen In the latter case please ensure...

Страница 85: ...the Conditions in the Sample in the Settings It is on the users authority to ensure that the sample is kept under this fixed temperature during the measurements Even if measurements with all activated...

Страница 86: ...recorders data acquisition systems Further the FireStingO2 can be operated in a so called Auto Mode in which the FireStingO2 performs measurements autonomously without any PC connected to it The auto...

Страница 87: ...oxygen channels The background is that the analog outputs are not fixed to specific oxygen channels ensuring highest flexibility Each analog output can be freely mapped to the oxygen Channel 1 4 The o...

Страница 88: ...mV for 0 O2 and 2200 mV for 21 O2 The second operation mode of the analog output is Alarm if out of range In this mode the analog output can output only two possible voltages either 0 mV or 2500 mV s...

Страница 89: ...be read out e g by an analog data logger from the analog output see chapter 11 1 NOTE Some older micro USB charger do not fulfill the new common standard for micro USB chargers Such an old micro USB...

Страница 90: ...ates digital data transmission via the UART interface of the extension port details on request NOTE Only sensors e g oxygen channel 1 4 temperature pressure humidity which are activated in the Pyro Ox...

Страница 91: ...igger input of the extension port see appendix 12 2 The advanced auto mode can be configured by opening the Settings window and clicking there on the Advanced Auto Mode button in the Options tab A sep...

Страница 92: ...ng Enable Serial Port Data Transmission the results for the respective channel are additionally transmitted via the UART interface of the extension port Ref Count is an advanced feature only relevant...

Страница 93: ...Non condensing conditions Oxygen channels 1 2 or 4 dependent on model Oxygen measuring principle lifetime detection of REDFLASH indicator luminescence Oxygen channel connector fiber optic ST plug Exci...

Страница 94: ...port X1 0 to 2 5 VDC 12 bit resolution Note slight non linearities around 0 5mV Analog Output 4 channels at extension port X2 0 to 2 5 VDC 14 bit resolution Note slight non linearities around 0 5mV Co...

Страница 95: ...ireStingO2 should not be powered via the USB port Pin 3 USB_DISABLE should be tied to pin 1 GND if the FireStingO2 should be operated in full control mode see below The transmit and receive pins of th...

Страница 96: ...7 and pin 2 VCC The trigger input is used for triggering a measurement in the triggered auto mode The trigger is activated at the moment when the pin is tied to GND Alternatively this pin can be used...

Страница 97: ...By closing the blue switch between pin 6 and pin 1 the auto mode is activated Note if the FireStingO2 is powered by a micro USB charger then the auto mode is automatically activated see chapter 11 an...

Страница 98: ...o the preceding case only the power supply is now given at pins 1 and 2 The USB connector of the FireStingO2 is left unconnected Note the additional connection between pin 1 and 3 powering down the in...

Страница 99: ...ngO2 Otherwise unintended data communication via a potentially still connected PC might disrupt the UART communication More information is available on request Connecting an external sensor voltage to...

Страница 100: ...iption 1 GND Ground 2 AO_A Analog Output 0 2 5 V DC 14 bit resolution Analog Output Port A alternatively digital alarm output 3 AO_B Analog Output 0 2 5 V DC 14 bit resolution Analog Output Port B alt...

Страница 101: ...rrounding and or decrease Amplification in the Advanced settings and or decrease LED Intensity in the Advanced settings Low Signal Sensor signal is too low check whether the sensor cable is connected...

Страница 102: ...he sensor tip or surface The REDFLASH indicators are excitable with red light more precisely orange red at a wavelength of 610 630 nm and show an oxygen dependent luminescence in the near infrared NIR...

Страница 103: ...e to the excellent luminescence brightness of the REDFLASH indicator the actual sensor matrix can be now prepared much thinner leading to fast response times of the PyroScience oxygen sensors The meas...

Страница 104: ...FireStingO2 So if you want to operate e g 6 different FireStingO2 meters you have to start the Pyro Oxygen Logger software 6 times which will open 6 Pyro Oxygen Logger windows on your desktop The dif...

Страница 105: ...value Definition raw value O2 uncalibrated The unit raw value is the default unit for uncalibrated sensors and shows only qualitative oxygen sensor readings partial pressure pO2 hPa mbar Used in gas a...

Страница 106: ...oncentration C mol L Definition C mol L A a s 100 x C100 T P S Used in water phase with C100 T P S interpolation formula for dissolved oxygen concentration in units of mol L at temperature T atmospher...

Страница 107: ...in units of PSU practical salinity unit g L In order to correct these values for the actual atmospheric pressure patm the following formula has to be applied C100 T P S C100 T P 1013mbar S x patm 101...

Страница 108: ...5 5 258 3 235 5 216 1 199 3 184 6 18 403 2 354 0 314 4 282 1 255 3 232 8 213 7 197 2 182 7 20 397 7 349 3 310 4 278 6 252 3 230 2 211 4 195 1 180 8 22 392 2 344 7 306 5 275 2 249 3 227 6 209 1 193 0 1...

Страница 109: ...Code XB7 532 205 Sensor Type LED Intensity Amplification Factory Calibration C0 Factory Calibration C100 Sensor Type Z Oxygen Micro Minisensor normal range Y Oxygen Minisensor normal range X Robust O...

Страница 110: ...C0 Factory Calibration at 0 O2 dphi0 C0 10 C100 Factory Calibration at 100 O2 dphi100 C100 10 The values of the factory calibration are valid for the following calibration conditions Partial Volume of...

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