Promega QuantiFluor E6090 Скачать руководство пользователя страница 2

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Promega Corporation ·

2800 Woods Hollow Road

Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526

Phone 608-274-4330

Fax 608-277-2516

· www.promega.com

Printed in USA.

Part# TM338

Revised 1/11

Page 1

Introduction

...................................................................................................................................................................2

Description...........................................................................................................................................................2

Product Components .........................................................................................................................................2

QuantiFluor™ Handheld Fluorometers General Specifications .................................................................4

QuantiFluor™ Handheld Fluorometers Optical Specifications ..................................................................4

Inspection.............................................................................................................................................................4

Precautions and Special Instructions...............................................................................................................5

Environmental Requirements ...........................................................................................................................7

Setting up the QuantiFluor™ Handheld Fluorometers

.......................................................................................7

Instrument Calibration and Operation

....................................................................................................................9

Instrument Power Up and Shut Down ...........................................................................................................9

LCD .......................................................................................................................................................................9

Detection Channel Configuration ....................................................................................................................9

Calibration Overview.......................................................................................................................................10

Calibration .........................................................................................................................................................10

Setting Calibration Standard Value ...............................................................................................................11

Performing the Calibration .............................................................................................................................11

Sample Analysis................................................................................................................................................12

QuantiFluor™ PCR Tube Adapter Operation .............................................................................................13

Diagnostic Information ....................................................................................................................................13

Solid Standard ...................................................................................................................................................14

Internal Data Logging (IDL)

....................................................................................................................................15

Activating IDL...................................................................................................................................................15

Downloading Data to a Computer ................................................................................................................15

Erasing Data from the QuantiFluor™ Instrument ......................................................................................15

General Considerations

............................................................................................................................................16

Handling Samples.............................................................................................................................................16

Linear Range and Quenching .........................................................................................................................16

Temperature ......................................................................................................................................................16

Sample Position.................................................................................................................................................17

Data Quality ......................................................................................................................................................17

Periodic Cleaning and Maintenance..............................................................................................................17

Troubleshooting

.........................................................................................................................................................18

Internal Data Logging (IDL)

....................................................................................................................................20

Shipping Checklist............................................................................................................................................20

Hardware Requirements for IDL ...................................................................................................................20

Installing IDL Software....................................................................................................................................20

Connecting the QuantiFluor™ Fluorometer ................................................................................................20

Troubleshooting Internal Data Logging........................................................................................................21

All technical literature is available on the Internet at: www.promega.com/tbs/

Please visit the web site to verify that you are using the most current version of this Technical Manual.

Please contact Promega Technical Services if you have questions on the use of this product. Email: [email protected]

QuantiFluor™ Handheld Fluorometers
Operating Instructions

Содержание QuantiFluor E6090

Страница 1: ...T e c h n i c a l M a n u a l QuantiFluor Handheld Fluorometers Operating Instructions INSTRUCTIONS FOR USE OF PRODUCTS E6090 E6105 AND E6100 PRINTED IN USA Revised 1 11 Part TM338 8772TA...

Страница 2: ...Fluor PCR Tube Adapter Operation 13 J Diagnostic Information 13 K Solid Standard 14 4 Internal Data Logging IDL 15 A Activating IDL 15 B Downloading Data to a Computer 15 C Erasing Data from the Quant...

Страница 3: ...er Features Compliant with the following EU directives 2004 108 EC Electromagnetic Compatibility 2006 95 EC Low Voltage Directive Comprehensive technical support 1 B Product Components Product Quantit...

Страница 4: ...1 Setup Guide 1 CD containing Technical Manual Product Quantity Cat QuantiFluor P Handheld Fluorometer Green Blue 1 each E6100 For Research Use E6600 Filters Channel A Green 525nmEx 570nmEm Channel B...

Страница 5: ...nts QuantiFluor ST AC Adapter 100 240V AC 50 60 Hz 0 25A Instrument 9V DC 0 3A QuantiFluor P 4 AAA Batteries 1 D QuantiFluor Handheld Fluorometers Optical Specifications 1 E Inspection Upon receiving...

Страница 6: ...l concernant la destruction de l appareil Veuillez respecter les exigences de votre tablissement concernant la destruction des accessoires Equipment protected by double insulation or reinforced insula...

Страница 7: ...Sample Compartment cannot accept cuvettes with an outer diameter over 12mm The 10mm size refers to the internal diameter of the cuvette so some plastic cuvettes may be too large Do not force oversize...

Страница 8: ...Figure 1 Slide the desired AC plug onto the Adapter from the top until it clicks into place Figure 1 Placing the plug on the AC Adapter Depress the button immediately underneath the plug arrow 1 and...

Страница 9: ...lightly lubricate the o ring if necessary Figure 3 Back Panel of the QuantiFluor P Fluorometer showing the battery compartment Figure 4 Front Panel of the QuantiFluor ST Handheld Fluorometer The cont...

Страница 10: ...B LCD The QuantiFluor Fluorometer has a backlit adjustable brightness LCD Liquid Crystal Display for easier viewing The contrast may change with temperature and may get lighter as the unit gets colde...

Страница 11: ...rometer and accepts maximum total volume of 250 l 2 The 10 10mm square methacrylate or polystyrene cuvettes require a minimum volume of 2ml 3 Be sure to insert the Minicell Adapter in the correct orie...

Страница 12: ...uorometer will average the reading for 10 seconds and set the zero blank point During this time the screen will display Reading Blank 4 After the instrument has set the zero point the screen will disp...

Страница 13: ...verage the fluorescence signal over a 5 second interval During this time the instrument screen will indicate Reading The result will be displayed at the top center of the screen 6 Once the Reading mes...

Страница 14: ...0 5 ml PCR tube The minimum volume required for reproducible results is 100 l Insert the PCR tube into the PCR tube Adapter close the lid and measure the sample Take care not to introduce air bubbles...

Страница 15: ...t settings have been changed 1 After calibration insert the Solid Standard into the sample compartment of the QuantiFluor ST Fluorometer Note always insert the Solid Standard with the tab away from yo...

Страница 16: ...stalled See Section 7 for computer requirements and installation instructions 1 Connect the QuantiFluor Fluorometer to the computer serial port using the provided interface cable 2 Open the Spreadshee...

Страница 17: ...tte wall often will help dissipate bubbles 5 B Linear Range and Quenching The linear range is the concentration range in which the QuantiFluor Fluorometer readout is directly proportional to the conce...

Страница 18: ...calibrate it It is important to take care when preparing standards samples and blank Follow the appropriate laboratory practices when preparing all solutions and reagents 5 F Periodic Maintenance and...

Страница 19: ...ed correctly Protect sample from light Some fluorophores are susceptible to photodegradation Make sure the correct optical channel is selected Make sure that the instrument is properly calibrated befo...

Страница 20: ...inear range of detection Fluctuating results Use the Solid Standard to determine if the instrument is functioning properly Check for spills in the sample compartment If liquid is spilled inside the sa...

Страница 21: ...programs 2 Insert the CD and run the setup program 3 The setup wizard will install the necessary files When the setup is complete a SPREADSHEET INTERFACE SOFTWARE icon will appear in the PROGRAMS men...

Страница 22: ...ument or program could be occupying the port making it unavailable Make sure to close all programs of this type before downloading data The port selected is incorrect Click on the box to the right of...

Страница 23: ...button to begin reading blank Reading Blank XX X Insert Cal Soln then press ENT Press ENTER button to begin reading cal solution Calib Complete press ENT Press ENTER button to accept calibration If E...

Страница 24: ...TER button Insert sample and press the ENTER button Reading BLUE WAIT XXX X BLUE Once WAIT disappears another sample can be read XXX X BLUE Press the ENTER button Press the ENTER button Press the ENTE...

Страница 25: ...th corrosive materials or atmosphere is not covered by warranty Damage from salts proteins and other moderately corrosive materials that are not promptly removed from the instrument are not covered by...

Страница 26: ...viced Select either A or B A I confirm that the returned items have not been contaminated by body fluids or by toxic carcinogenic radioactive or other hazardous materials B I confirm that the returned...

Страница 27: ...com 2 Carry out minor adjustments or tests as suggested by your Technical Services contact 3 If it is determined that the instrument should be returned for repair Promega Technical Services will arra...

Страница 28: ...it performing to original factory specification Product Quantity Cat Minicell Borosilicate Glass Cuvettes 1 pkg of 400 E6091 replacement cuvettes 10 10mm square Polystyrene Cuvette 3 5ml capacity 1 pk...

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