Promega Corporation ·
2800 Woods Hollow Road
·
Madison, WI 53711-5399 USA
·
Toll Free in USA 800-356-9526
·
Phone 608-274-4330
·
Fax 608-277-2516
· www.promega.com
Printed in USA.
Part# TM338
Revised 1/11
Page 11
3.F. Setting the Calibration Standard Value
These directions define the numeric value for the standard to be read. The units of measurement are set
in µg/L. For example if a Primary Standard has a 50µg/L concentration, then set the value to 50, and the
implied unit of measure will be in µg/L.
1.
Press the
STD VAL
button to view the value of the standard currently set. The screen will
display “Cal Val: XX.X <UP/DOWN> BLUE”.
Note:
Be quick. After after 5 seconds, the display defaults to home. This standard value will
apply only to the channel you have previously selected using the
A/B
button (Section 3.C).
2.
Press the
UP ARROW
or
DOWN ARROW
buttons to set the standard value. Holding down
either of the arrow buttons will result in faster scrolling of the value.
3.
When you have set the standard value, press the
ENTER
to accept the value and return to
the HOME screen.
3.G. Performing the Calibration
1.
Press th
e CAL
button, the screen will display “Calib BLUE <ENT> to start”.
2.
Press the
ENTER
button to move to the next screen, which will display “Insert Blank then
press <ENT>”.
3.
Insert the blank sample, and press the
ENTER
button. The QuantiFluor™ Fluorometer will
average the reading for 10 seconds and set the zero (blank) point. During this time the screen
will display “Reading Blank”.
4.
After the instrument has set the zero point, the screen will display “Insert Cal Soln then press
<ENT>”.
5.
Insert your standard sample and press the
ENTER
button.
6.
The average reading is taken for 10 seconds, and the Standard Calibration value is set. Once
the value is set, the screen will display “Calib Complete press <ENT>”.
Note:
If the instrument was calibrated with the highest concentration standard, and the RFU
of the standard is too close to that of the blank, the instrument will register a
"Standard<Blank Recalibrate" message. The RFU of the standard (RFU-STD) must be at least
3X greater than the RFU of the blank (RFU-BLK) in order to calibrate the instrument. If the
"Standard<Blank Recalibrate" message appears, we recommend increasing the standard
concentration by 10X or examining the blank for high fluorescence background.
7.
Press the
ENTER
button to accept the calibration. If the
ENTER
button is not pressed within
10 seconds, a message will display asking if you wish to abort the calibration. Press the
UP
ARROW
to abort the calibration or the
DOWN ARROW
to accept it. If you abort the
calibration, the display will return tot he HOME screen, and the instrument will default to the
previous calibration.
Note:
At any time during Steps 1-4, you can press the
ESC
button to stop the calibration. The
display will return to the HOME screen, and the instrument will default to the previous
calibration.
