Promega GloMax GM2000 Скачать руководство пользователя страница 49 | Manualshive

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Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516

TM470 · 4/16

www.promega.com

6.B. Common Problems (continued)

Symptoms

Causes and Comments

Moisture appears on top of the Microplate Sample

The injector tips are bent. See Sections 5.D and 5.I for

Tray cover after a run.

instructions to change the tubing and clean the Optical
Head.

Injector tip holder is not seated correctly. Injector speed is
not set correctly, causing reagent drips.

The 96-well plate has overflowed inside instrument. • See Section 5.D for instructions to clean the Optical

Head.
• Check the wells selected for injection and measurement.
• Check the total injection volume in the software protocol.
• Check the volume of sample inside the well. The
maximum volume per well is 300µl.
• Thoroughly clean the interior of the instrument.

Reagent has spilled inside the instrument

Wipe up spills immediately using an absorbent paper towel
or Kimwipes

®

tissue. If necessary, use a 70% ethanol

solution to remove reagent residue.

6.C. Use of a USB Flash Drive

•

Insert a USB flash drive into one of the two USB ports on the back of the instrument before running any proto-
col and do not remove until the protocol is finished.

•

Do not insert or remove USB flash drive while instrument is running.

•

Because USB flash drives may vary from vendor to vendor or from type to type, incompatibilities may occur.
If the USB flash drive is not detected after a few seconds or if you encounter any issues with the USB flash
drive, shut down and restart the Tablet PC and the instrument, and try a different brand of USB flash drive.

•

If the Tablet PC becomes unresponsive after inserting a USB flash drive, shut down the Tablet PC and
instrument, remove the USB flash drive, and restart the Tablet PC and the instrument.

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Содержание GloMax GM2000

Страница 1: ...4 16 TM470 GloMax Navigator System Instructions for Use of Products GM2000 and GM2010 O P E R A T I N G M A N U A L...

Страница 2: ...ng Up the GloMax Navigator Instrument and Tablet PC 10 2 B Switching On the GloMax Navigator Instrument 11 2 C Shutting Down the GloMax Navigator Instrument 12 2 D Operating the GloMax Navigator Syste...

Страница 3: ...glow and flash based luminescent reactions in 96 well plates The instrument is operated by an integrated Tablet PC which provides quick and easy navigation through the control options Exporting your...

Страница 4: ...ately 40lb 18 1kg Power Requirements 0 5A 100 240V AC 50 60Hz universal Warranty 1 year parts and labor warranty included Regulatory Compliance For Research Use Only Not for use in Diagnostic Procedur...

Страница 5: ...of luciferase Dynamic Range 9 logs Cross Talk Less than 3 10 5 white 96 well plate Corning Cat 3912 Injector System Specifications Number of Injectors optional Two injectors Dispense Volume Range 5 20...

Страница 6: ...gator has already been configured for the settings listed above 1 C Product Components P R O D U C T S I Z E C AT GloMax Navigator System 1 each GM2000 For Research Use Not for use in Diagnostic Proce...

Страница 7: ...to the equipment or surrounding area Avertissement Risque de pr judice corporel pour l op rateur ou d accident avec l instrument ou l entourage 6687TA eps It is important to understand and follow all...

Страница 8: ...void the user s authority to operate the equipment Do not use this device in proximity to sources of strong electromagnetic radiation e g microwave oven because they may interfere with the proper oper...

Страница 9: ...x Navigator n est pas en cours d utilisation 5295MA eps The GloMax Navigator is intended for indoor use only Le GloMax Navigator est destin une utilisation en int rieur 5295MA eps Always disconnect th...

Страница 10: ...vice If any item is damaged contact Promega Technical Services E mail techserv promega com Standard components are shown in Figure 2 Panel A below Figure 2 GloMax Navigator System Accessory Components...

Страница 11: ...nt and Tablet PC 1 Unpack the Tablet PC from its shipping box 2 Place the Tablet PC in the Tablet Holder on the top of the GloMax Navigator Instrument in the orientation shown in Figure 4 Fi 13516TA g...

Страница 12: ...ure 6 The back of the GloMax Navigator Instrument A On Off switch B Power connection C USB port to connect to Tablet PC D Two USB ports 2 B Switching On the GloMax Navigator Instrument 1 Switch the in...

Страница 13: ...or Software by tapping on the x in the upper right corner Figure 7 3 Shut down the Tablet PC using normal Windows 8 shutdown procedures Swipe in from the right edge of the screen select Settings then...

Страница 14: ...ng inside the text box and entering a name Figure 9 13596TA Figure 9 Save Results Window 6 Select Start to begin the protocol 7 The protocol will begin acquiring data and will display the results in t...

Страница 15: ...button will provide you a list of all available protocols These include preloaded protocols as well as user defined protocols The user can create user defined protocols from this screen as well Smart...

Страница 16: ...ectors to Prime Reverse Purge and or Flush and accessing the instrument information About Eject Tapping this button will move the Plate Tray to the Home position to add or remove a plate from the inst...

Страница 17: ...g All Favorite protocols can be marked by tapping the Star adjacent to the protocol name After marking a protocol as a favorite those protocols will automatically be listed at the top of the Protocol...

Страница 18: ...trument 3 Tap OK when ready to begin the protocol The protocol will begin when OK is selected so ensure the plate has been loaded into the instrument 4 When the read is complete the user will be promp...

Страница 19: ...New Protocol button at the bottom of the screen Figure 12 2 You will be prompted to name the protocol You also may provide a description if desired 11630TA Figure 15 New Protocol name prompt from the...

Страница 20: ...n the bottom right corner of the screen A user message will appear indicating that the protocol was saved successfully F 11633TA igure 18 GloMax Navigator New Protocol Successfully Saved user message...

Страница 21: ...ified whereas the plate maps within each step of the protocol are now active and the user can modify each step as desired Figure 13605TA Plate Map is unlocked and cannot be applied Select Plate Map fo...

Страница 22: ...tocol Window The functions can be expanded and contracted by selecting the black triangle at the top left corner of each function They may be removed by selecting the black X at the top right corner o...

Страница 23: ...200 l and the injection speed 20 l sec to 500 l sec Select the wells that will be injected by touching the plate map and selecting the desired wells Warning Make sure sufficient space is available in...

Страница 24: ...is checked the Prompt screen shown in Figure 25 appears to the user during protocol with the option to open the door If Eject Plate is selected the instrument will home and the user can then open the...

Страница 25: ...um of 100 loops can be performed within a single protocol 1 Add the Loop step to the protocol by dragging and dropping the Loop icon into the protocol window F 13611TA igure 26 Loop function 2 Select...

Страница 26: ...ocol Window 13613TA Figure 28 Kinetics function 2 All well associated operations within the kinetic block must have the same well selection The plate maps of these operations are not individually edit...

Страница 27: ...the results 13615TA View results within a group by sorting on date time name or user Export or remove 1 or more results Select to remove or export file Figure 30 GloMax Navigator Results Screen 2 To...

Страница 28: ...view results Figure 32 GloMax Navigator Results screen with heat map display Kinetic results display a graphical representation of the data during the course of the kinetics read Figure 33 Data are au...

Страница 29: ...d to a number of locations including a USB flash drive the Tablet PC C drive or a cloud based storage c Two files are automatically exported a comma separated value file and a Microsoft Excel workshee...

Страница 30: ...Excel Workbook Note Colors in the Excel workbook will not reflect those within the software heat map and therefore you will need to place the data into cells of similar shades if you wish to maintain...

Страница 31: ...21TA Figure 38 The GloMax Navigator Settings Screen Instrument Info Tapping the Instrument Info button reveals a list that includes the software and firmware versions the instrument name and maintenan...

Страница 32: ...r Instrument Injector System Specifications Number of Injectors Two Injectors Dispense Volume Range 5 200 l in 1 l increments Injection Speed 20 500 l second Waste Collection Tray Volume Approximately...

Страница 33: ...nt axes itself when either the Door button is selected a Protocol is started or if the instrument power is turned Off On Initialize all axes by selecting the Start button in this screen 13633TA Figure...

Страница 34: ...for the instrument F 13624TA igure 44 Settings Administrator screen Export Folder Select the folder location to which the data should be exported Once this is configured the data will be exported to t...

Страница 35: ...new protocols with instructions directly from the Promega web site www promega com resources tools detection instruments software promega branded instruments glomax navigator Download the protocol fi...

Страница 36: ...e of the screen by Today This Week the past 3 Months the past 6 Months This Year or by All Detailed audit records are automatically generated and stored with the GloMax Navigator software that cannot...

Страница 37: ...uires injectors the injectors must first be primed with at least 500 l of the reagent to be injected Priming wets the fluid path and removes air bubbles to ensure delivery of an accurate injection vol...

Страница 38: ...he injector tube through the Para lm and into the reagent bottle 4 Ensure the Waste Collection Tray is in position and empty 5 Tap the Start button adjacent to the Prime Injectors information in Figur...

Страница 39: ...ents left in the fluid lines will be returned to the reagent container 4 C Flushing the Injectors after Use 1 Remove the sample plate from the instrument 2 Tap the Start button adjacent to the Flush o...

Страница 40: ...his may cause damage to the instrument and its electronics Do not use solvents or abrasive cleaners Use a cloth dampened with deionized water to periodically wipe clean the exterior of the GloMax Navi...

Страница 41: ...f the instrument Figure 54 Fi 13542TC Optical head Microplate sample tray and cover gure 54 Interior of the GloMax Navigator instrument 3 Loosen the hand screws and remove the Injector Tip Holder from...

Страница 42: ...to air dry completely before proceeding Alternatively blot dry with a lint free Kimwipes tissue 7 Return the Aperture to the Optical Head Align the Aperture with the Optical Head and gently guide the...

Страница 43: ...re to properly maintain a clean interior may cause a mechanical error and the irretrievable loss of data We recommend thoroughly cleaning the interior every 30 days 1 Turn OFF the instrument 2 Use a K...

Страница 44: ...nto position 9 Place a container of deionized water on the bottle holder with the inlet tube inserted to reach the bottom of the container Prepare at least 10ml of deionized water for each injector to...

Страница 45: ...Tip Holder to the Optical Head and tighten the hand screws Position the holder just above the front pins on the Optical Head Push the holder toward the right to lock the holder securely to the right...

Страница 46: ...the instrument has been opened unexpectedly during operation of the instrument Manually opening the door of the instrument during an instrument operation will cause that operation to be aborted Refer...

Страница 47: ...tion 4 C Injector speed may not be optimal for the viscosity or composition of the solutions used Injectors not injecting No reagent in injector lines Confirm that the inlet tubing is inserted into th...

Страница 48: ...Low activity in sample Use white opaque plates High luminescent cross talk Use high quality white or black opaque plate Injector leaks The inlet or outlet tubing is not properly connected to the inje...

Страница 49: ...is 300 l Thoroughly clean the interior of the instrument Reagent has spilled inside the instrument Wipe up spills immediately using an absorbent paper towel or Kimwipes tissue If necessary use a 70 et...

Страница 50: ...rogram please ensure it is set to manual not automatic update Antivirus software will slow the performance of the Tablet PC and will affect the performance of the GloMax Navigator Instrument while it...

Страница 51: ...ate time zone for your location and whether you wish the clock to automatically adjust for Daylight Savings Time e Tap the OK button to save the time zone settings f You will be returned to the Date a...

Страница 52: ...er The Local Users and Groups dialog should appear 2 In the left hand pane of the Computer Management window select Local Users and Groups and double tap Groups Note If the Local Users and Groups menu...

Страница 53: ...nt to add users tap PromegaUsers A PromegaUsers window will open which can be used to add existing users to the PromegaUsers group Tap the Add button to add users to the PromegaUsers group In the Ente...

Страница 54: ...ting users from the PromegaAdministrators group 4 Select the user you want to remove and tap the Remove button Tap OK to remove selected user from the PromegaAdministrators group Close the Computer Ma...

Страница 55: ...ment name from the Instrument Name Window 7 B Icons and Symbols Home screen 11609TA Exit Software 11611TA Quick access to the Tools Screen 13629TA Home all axes 13630TA Help Screen From any screen oth...

Страница 56: ...on contact Promega Technical Services Contact information is available at www promega com E mail techserv promega com To obtain service during the warranty period please take the following steps 1 Con...

Страница 57: ...ed by toxic carcino genic radioactive or other hazardous materials B I confirm that the returned items have been decontaminated and can be handled without exposing personnel to health hazards Circle t...

Страница 58: ...avigator Parts Only Service Agreement 1 each SA1302 GloMax Navigator Basic Service Agreement 1 each SA1303 GloMax Navigator Preventive Maintenance 1 each SA1308 GloMax Navigator Installation Qualifica...

Страница 59: ...System 10ml E2510 Renilla Luciferase Assay System 100 assays E2810 Renilla Glo Luciferase Assay System 10ml E2710 ADCC Reporter Bioassay Core Kit 1 each G7010 ADCC Reporter Bioassay Core Kit 5X 1 each...

Страница 60: ...NanoBiT ONE Glo P450 Glo and ToxGlo are trademarks of Promega Corporation Windows is a registered trademarks of Microsoft Corporation Kimwipes is a registered trademark of Kimberly Clark Corporation...

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