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TM470 · 4/16
Kinetics Function
The Kinetics tool is used to perform a set of steps on a
per-well basis
, then repeating the set of steps a desired
number of times (i.e., inject and read one well multiple times before proceeding to the next well).
Note:
Kinetics can be used with all protocol functions except for user prompt functions. Loops may not be placed
within a kinetic step, but kinetic steps may be placed within Loops. A maximum of 250 kinetic reads in 1-unit
increments can be performed within a single protocol. For example, 10 readings with a 1-second interval means
that 1 reading is taken every 1 second for 10 seconds.
1.
Add the Kinetics step to the protocol by dragging and dropping the
Kinetics icon
onto the
Protocol
Window
.
13613T
A
Figure 28. Kinetics function.
2.
All well-associated operations within the kinetic block must have the same well selection. The plate maps of
these operations are not individually editable.
3.
Begin building the desired workflow by dragging and dropping the desired steps into the kinetic step. They
should be contained within the kinetic step.
F
13614T
A
igure 29. Kinetic Function with a Luminescence step within the Kinetic container.
4.
Select the number of readings and interval (seconds) between each reading.
Note:
Typically the protocol steps are completed before the completion of the
Interval Time
, and therefore
the instrument will pause until the completion of the
Interval Time
duration. However, if the protocol steps
take longer to complete than the
Interval Time
, the protocol will complete all steps and then immediately
continue without pausing for the
Interval Time
duration. Otherwise, once all steps are completed the protocol
will pause until the
Interval Time
duration has been reached.
5.
Rearrange the steps by touching and dragging the step.
