Princeton Instruments ProEM+ EMCCD, Руководство пользователя

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Chapter 5 Operation 57
57
will be active. The Status bar (at the bottom of the window) displays an icon for
temperature status which reports the current temperature and whether the set
temperature has been reached. Clicking on the icon opens the
Sensor expander
where the set temperature can be changed.
3. On the Shutter expander, select Shutter Mode: Normal .
4. Open the Spectrometer expander, select the appropriate grating. In this case,
the 300g/mm (Blaze: 750) grating was selected and the center wavelength was
set to 500 nm for a mercury lamp. Use 0.0 nm if using a broadband source.
Confirming the Setup
1. Turn on the light source at the spectrograph entrance slit.
2. Click on Run to begin previewing the data. Depending on the display settings,
you should see either a spectral band (image) or a graph. Background noise will
decrease as the camera cools to its default temperature.
3. Turn off the light source. The data display should change to a background noise
pattern or low intensity graph. If this occurs you have confirmed that light
entering the spectrograph is being seen by the camera. Skip Steps 4-5 and
continue to the
“Rotational Alignment and Focusing”
topic on page 58.
4. If there is little or no difference between the data displayed when the light source
is on or off:
a. Verify that the camera’s manual shutter is open (i.e., the shutter knob should
be pushed in).
b. Verify that the light source has power and is turned on.
c. Verify that the entrance slit is open at least 10 µm.
d. Check the Exposure Time ( Common Acquisition Settings expander ) .
e. Confirm that Shutter Mode is set to Normal ( Shutter expander ) .
f. Check the shutter cable connections.
g. Verify shutter operation. You should hear the shutter open and close while
Run is active.

If you hear a shutter operating and you have done Steps a-f, turn the light
source on, wait a minute and then turn the light off while you view the
data display. If the problem is fixed, stop acquisition or continue to the
“Rotational Alignment and Focusing,”
topic on 58.

If you do not hear a shutter operating and you have done Steps a-f, stop
data acquisition and continue to Step h.
h. Make sure the spectrograph has an entrance slit shutter. An externally
mounted shutter is easily confirmed. Verifying an internally mounted shutter
requires access to the inside of the spectrograph: refer to the spectrograph
manual for instructions.
5. If you need more help, contact Customer Support. See page 146 for contact
information.