Princeton Instruments ProEM+ EMCCD, Руководство пользователя

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54 ProEM
®
+ System Manual Version 1.B
Figure 26. View Area Displaying an Image
3. Adjust the lens aperture, intensity scaling, and focus for the best image as viewed
on the computer monitor. Some imaging tips follow:

Begin with the lens blocked off and then set the lens at the smallest possible
aperture (largest f-stop number).

Make sure there is a suitable target in front of the lens. An object with text or
graphics works best.

Check the brightest regions of the image to determine if the A/D converter is at
full-scale.
A 16-bit A/D is at full scale when the brightest parts of the image
reach an intensity of 65535. Adjust the aperture to where it is just slightly
smaller (higher f-stop) than the setting where maximum brightness on any part
of the image occurs.

Set the focus adjustment of the lens for maximum sharpness in the viewed
image.
4. After you have focused the camera, you can stop Preview mode, continue
Preview
mode, begin
Acquire
mode, wait for the CCD to reach the operating
temperature before going to Acquire mode, or you can shut down the system.
Shutdown
1. Close LightField.
2. Turn off the camera power.