PlexBio IntelliPlex IW100, Руководство пользователя

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IntelliPlex
TM
1000

Code User Manual - Jan-20
Fig. 24. Positions of the Washing and SA-PE Tanks
5.5.2 Wash Buffer
This section describes the procedures for Wash buffer preparation.
Preparation of Wash buffer is required for all built-in PlexBio assays. Before starting a run,
sufficient amount of Wash buffer must be added to the Wash Buffer bottle connected to the
IntelliPlex 1000 πCode Processor.
Wash buffer purchased from PlexBio is 10X concentrated. Preparation of 1X Wash Buffer (10-fold
dilution of 10X Wash buffer with ddH
2
O) is required before usage with the IntelliPlex 1000 πCode
Processor. The prepared 1X Wash Buffer can be used for up to 1 week.
Do not use the buffer if visible contamination (e.g. fungus) occurs. Please discard of contaminated
Wash Buffer according to local requirements and wash the fluid path and buffer bottles several
times with 70% ethanol (refer to section 7.2.1).
In a custom program, the amount of wash buffer required per row depends on the selection of
program parameters (such as number of wash cycle and selection of “clean tip” option). We
recommend a fill level of at least 500 mL Wash Buffer for all assays with 7 or less wash cycles and
a fill level of 1000 mL Wash Buffer for programs utilizing more than 7 wash cycles.
For PlexBio’s DNA/RNA program, the Wash Buffer consumption is as following:
Front: SA-PE Tank (V Tray)
Back: Washing Tank (U Tray)