PlexBio IntelliPlex IW100, Руководство пользователя

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22
IntelliPlex
TM
1000

Code User Manual - Jan-20
SA-PE Reaction:
This process includes the fluorescence labeling step with automated SA-PE
dispensing. A fixed volume of 50

L SA-PE will be dispensed into each well,
then incubate at 37°C for desired incubation period followed by 4 washing
cycles. At the end, additional 150

L of wash buffer will be dispensed into
each well, followed by 10 sec. of shaking to spread πCode evenly. The plate
will now be ready for fluorescence analysis using the PlexBio 100 Fluorescent
Analyzer.
The incubation period can be adjusted from 1 sec to 23hr 59min 59sec.
Please ensure that enough SA-PE solution is added into V Tray before the
program starts. See Section 5.5 Special Applications for more detail.
Fig. 17. Parameters of custom SA-PE procedure
Note: The IntelliPlex 1000 πCode Processor dispensing module will take up 50

L of solution from
the V Tray to dispense into selected wells. By default, the V Tray is used for SA-PE. Custom
programs may also utilize the V Tray to dispense 50

L of a solution of choice as long as the
reaction is compatible with an incubation at 37°C. For example, immunoassays may dispense
Phycoerythrin-labbeled detection antibodies instead of SA-PE.