Comments:
PerkinElmer LAS Representative:
Date:
Reviewed By:
Date:
Flexar Analytical / FX-10 UHPLC Pump
IQ/OQ Rev. D June 2009
•
Record mV or absorbance values from the run for each step (AU1 through AU6, and if
applicable, for the quaternary pump, AU7 through AU10) in the AU column of Tables 1
and 2 below.
Table 1 - Binary and Quaternary (Reservoirs A and B)
STEP
TIME
FLOW
%A
(water)
%B
(acetone)
CURV
AU
(absorbance or mV)
0
5.0
5.0
100
0
0.0
1
1.0
5.0
100
0
0.0
2
3.0
5.0
0
100
0.0
AU1 =
3
3.0
5.0
10
90
0.0
AU2 =
4
3.0
5.0
49
51
0.0
AU3 =
5
3.0
5.0
51
49
0.0
AU4 =
6
3.0
5.0
90
10
0.0
AU5 =
7
5.0
5.0
100
0
0.0
AU6 =
Table 2 - Quaternary (Reservoirs C and D)
STEP
TIME
FLOW
%C
(water)
%D
(acetone)
CURV
AU
(absorbance or mV)
0
5.0
5.0
100
0
0.0
1
1.0
5.0
100
0
0.0
2
3.0
5.0
0
100
0.0
AU7 =
3
3.0
5.0
10
90
0.0
AU8 =
4
3.0
5.0
90
10
0.0
AU9 =
5
5.0
5.0
100
0
0.0
AU10 =
