Comments:
PerkinElmer LAS Representative:
Date:
Reviewed By:
Date:
Flexar Analytical / FX-10 UHPLC Pump
IQ/OQ Rev. D June 2009
•
Purge the pump and fill the line to be used for the test with water.
•
Zero the scale. Weigh the collection vessel on a calibrated scale or balance.
•
Take the collection vessel and go to the pump and set the pump flow for 0.9 mL/minute.
•
Start the pump. (Direct the initial flow of water off to waste until the pressure stabilizes.
•
When pressure stabilizes, direct the flow of water into the collection vessel and
simultaneously start a stopwatch or other timing device.
•
Let the pump run for 10minutes.
•
When 10minutes has elapsed, remove the tube from above the collection vessel and
insert it into the waste.
•
Weigh the collection vessel with the water. Subtract the weight of the collection vessel .
•
The remaining weight of the water should be 9 grams ±
0.09 g (8.91 to 9.09g)
0.9 mL/min * 10 min = 9 g (water weighs 1 gram per mL)
•
Divide the weight of the water by 10 to get the volume delivered in a 1 minute interval.
•
Record this number in the Test Result field of Flow Accuracy @ 0.9 mL/min portion of the
IQOQ Test Certificate. Enter N/A if testing Analytical Pump
2.4.5
Test Results
Parameter
Specification
Test Result
Pass/Fail
Comments
FX-10
Pump (30
µ
µ
µ
µ
L
Piston)
Accuracy: 0.9
mL/min
±
1% of 0.9 mL/min
(0.891ml/min to
0.909ml/min)
