CyFlow
®
Cube Instrument Operating Manual
Rev-05_2014-01-31
9/40
G
M
L
Histogram and dotplot in immunology
Lysed blood 2D dotplot
Lysed blood is represented in a dot plot presenting the FSC in X axis and SSC in Y axis. Both axes are in a
linear scale.
Three distinct groups are visible; they represent the granulocytes (
G
), monocytes (
M
) and lymphocytes (
L
).
Histogram and dotplot of immunological staining
This histogram represents the spectrum of the cells presented in the
previous dot plot stained with antibodies anti-CD45 conjugated to
PhycoErytrin (PE) Dy647. The X axis displays the fluorescence in a 4-dec
logarithmic scale and the Y axis displays the number of events in a linear
scale.
This dot plot presents the cells fluorescence in X on a logarithmic scale
(CD45 PE-Dy647) versus SSC in Y on a linear scale.
This data display allows an easier interpretation of 2 parametric data
compared to the histogram. Concluded from this example, the lymphocytes
are strongly stained with the anti-CD45 antibody, the monocytes are slightly
stained and the population of granulocytes is negative.