CyFlow
®
Cube 8 - Instrument Operating Manual
Rev-05_2014-01-31
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Peaks
Number of events
Threshold
of lower
limit
Linear representation of SSC
Log representation
for fluorescence
Example of a histogram
A histogram represents a distribution of measured signals (events) over 1
dimension. Data can be presented on the dimensions of relative particle size or
optical particle structure (Forward Scatter (FCS) or Side Scatter (SSC)), resp.
or on their relative fluorescence intensities in different light colors (fluorescence
parameters FL1 to FL6).
In this example, the dimension represented is the relative size (FSC) on a
logarithmic scale in X, and the number of events on a linear scale in Y. Two
peaks are visible.
Example of a dotplot
A 2D dotplot presents correlated data over 2 dimensions. In the image on the right a sample of leukocytes
(after lyse of the red blood cells) is plotted with their relative light scattering (SSC) property against the
intensity of the CD45 antigen.
The Z value represents the number of events that have the same coordinates. 1 event will be represented
by a black point; if 10 events have the same coordinates, the point representing them will be grey. It will be
yellow if more than 20 points are overlaying one another. The Z scale is dynamic and will adapt during the
measurement to a scale of 1 (black) to the maximum overlaying event coordinate color coded in red.