CyFlow
®
Cube 8 - Instrument Operating Manual
Rev-05_2014-01-31
26/40
Region/ROI properties
Create a polygonal region
Double left click on the plot where the gate/region is required, a first point will be
set; a second click will set a further point, and so on. Label as many points as
required with the left mouse button. To close the gate surface, click mouse right.
Region properties
To access the region property,
Alt+right-click
on the gate to edited. On the
PLOTS
register on the right of
the screen a selection of region options is displayed.
Region name
As default, the gate will be named: region n
x
,
successive region: region n
x+1
The user can also define a name. Remember to
validate by
Accept
before to move to another region.
Home Plot name
This defines the physical plot where the region is
located.
Color RGB
Allows to choose the color of the region. This color will be reported to each plot selected as
color gating
only
.
Max count
This option allows fixing the maximum number of events from a particular ROI (Region Of Interest) to be
analysed (see CyView
™ 8 FILE register).
Sorter region
To set the ROI/region as a sorting gate, just by selecting the box.
(This option is only available on the Cube equipped with Sorter flow cuvette).
Moving of regions within a histogram
Move the cursor into the region and keep the left mouse button pressed while
moving the position. Individual points of a region can be changed by
approaching with the cursor to the point and keep the left mouse button
pressed during movement. Regions within a dotplot can be changed in size
by selecting the regio
n with the cursor and pressing the “Shift” button during
cursor movement
Each region can be selected with the right mouse button for deletion.