CyFlow
®
Cube Instrument Operating Manual
Rev-05_2014-01-31
17/40
ACQUISITION
– instrument control
Select the trigger parameter
multiple trigger parameter
s are logical “or” connections
Gain
values
to
change the voltage
of the individual optical
parameters
(0 volt - 999 volts) defines PMT signal amplification
Threshold
defines the trigger signals cut-off level
(on 4 dec log scale)
Flow
defines the speed of sample injection in µl/s
Lights on
switches light sources on / off
MEASURE
– definition of measure modes
Measmode
allows selection of:
Analyze all:
run sample until its levels reached the stop electrode
Volumetric counting (VC) with volume
a volume can be pre-selected
Volumetric counting (VC) with electrodes:
START and STOP electrodes of the sample port were
used to define a fixed sample volume of 200µl
Cells in region
a particle number can be pre-selected
Speed values
and
Volume values
can be edited and will be stored upon saving a confirmation file.
Autoflags
allows activation of specific functions:
Autostart: automatic start of the measurement
Activate the AutoStart flag
Press start (only ones to confirm the processes)
Connect a sample tube
With each new connection a sample tube the next measurement will be initialled until the button is
pressed.
AutoPA: automatic peak analyse
, at the end of the measurement → only for Ploidy
Autosave: automatic storage at the end of the measurement.
Autosave does not function when the measurement is finished by the
“end” button.