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http://www.parker.com/ppf

  

Page 6 

 
EXTERNAL INPUT

 

For additional flexibility in experimental use, the 
Picospritzer III has a front panel BNC jack for each 
channel which permits independent activation of 
the two channels from external sources. The 
selector toggle switch above each jack determines 
the source of the control signal for that channel. In 
the “EXTERNAL” position, it operates by 
energizing the valve whenever the input signal 
is high (+5 V.D.C.) and de-energizes it when the 
signal returns to ground. This allows for pulse 
durations longer than the capacity of the internal 
timer. In the “TIMER” position, the channel is 
connected to the internal timer and will activate 
whenever that timer is triggered (either manually or 
externally). 
 

SECOND CHANNEL 

The Picospritzer III is equipped with a separate 
BNC jack and selector toggle for each of the two 
channels. This allows 2 external signals to be used 
to operate the 2 channels independently when the 
selector toggles are set to the “EXTERNAL” 
position. Placing a selector toggle to the “TIMER” 
position connects that channel to the internal timer 
for operation whenever the timer is triggered (either 
manually or externally). To prevent a channel from 
triggering, place the selector switch in the 
“EXTERNAL” position and do not make a 
connection to the BNC for that channel. 
 
All Picospritzer III's are designed with second-
channel capability. 
 

MARKER 

The time mark provides a convenient indication of 
the duration of the pulse with respect to a biological 
signal much like that of the “artifact” associated 
with iontophoresis. It is a 5-volt TTL signal 
controlled by channel one. The time mark not only 
provides a useful indication of the duration of the 
pulse, but it may also serve as a sync-out signal for 
triggering the sweep of an oscilloscope, etc. 

 

 
 
 

 

HOLDERS FOR PRESSURE PIPETTES  

Standard pipette holders are available for pipettes 
with diameters of 1.0, 1.2, 1.5 and 2.0 mm. 
 
For prices and dimensions, see the Picospritzer 
Accessories list. 
 

No internal adjustment of the Picospritzer should be 
undertaken by the user without consulting the 
manufacturer. Detailed instruction will be provided 
if necessary. 

 

PICOSPRITZER III FAQ 

(Frequently Asked Questions): 
 
1. I used to purchase the vacuum loading unit is that 
still available?  
 

Currently, the vacuum loading unit is no longer 
available for purchase 
 

2. I used to purchase a 0-10 or 0-60psi regulator, are 
those options still available? 
 

Currently, we no longer offer the 0-10 or 0-60psi 
regulator, only configurations listed on page 11 are 
currently available 
 

3. If I purchase a two channel unit, can I trigger the 
two channels independently using two separate 
footswitches or push buttons? 
 

Currently, the Picospritzer III can only handle one 
footswitch or push button (the footswitch and push 
button control the internal timer). You can trigger 
them independently using an external trigger. Please 
see Second Channel section to the left.

 

 

4. If I purchase a two channel unit can, I provide 
separate pressures to the two valve boxes?  
 

Currently, the Picospritzer will only provide the same 
pressure to both valve boxes.

 

 
 

 

Содержание PICOSPRITZER III

Страница 1: ...er Hannifin Precision Fluidics Division 45 Route 46 East Pine Brook NJ 07058 973 575 4844 http www parker com ppf PICOSPRITZER III Manual Pressure Systems for Ejection of Picoliter Volumes in Cell Research ...

Страница 2: ...nspections of the connections in the pressure system should be made to ensure they are all still tight particularly after a period of inactivity or if the system is moved This instrument is intended for use in an indoor environment between 10 and 40 degrees Celsius 50 to 104 degrees Fahrenheit with no more than 90 humidity non condensing EQUIPMENT SPECIFICATIONS Power Requirements 100 to 240 Volts...

Страница 3: ... front panel push button external stimulator 5 volt or remote push button foot pedal The Picospritzer III pressure system was designed for rapid and reproducible ejections of picoliter to nanoliter volumes used in conjunction with intracellular or extracellular studies while avoiding the inherent desensitization of nerve cells which accompanies Iontophoretic methodology Intracellular applications ...

Страница 4: ...on it PLEASE NOTE An important detail in arranging the location of the solenoid in relation to the pipette holder is to maintain a loose coil in the interconnecting small bore 1 16 inch Teflon tubing to absorb or dampen the pressure pulse thus avoiding movement of the pressure pipette DO NOT stretch the tubing out tight between the valve housing and the pipette holder II OPERATING SPECIFICATIONS S...

Страница 5: ... held down It also restricts the interval between repetitive pulses initiated by the push button to approximately 200 milliseconds The PULSE indicator will light during the course of the pulse EXTENDED RANGE The basic Picospritzer is furnished with extended range timing controlled by a three position toggle switch located at the right side of the Duration thumbwheel The top position labeled MSEC s...

Страница 6: ...he duration of the pulse with respect to a biological signal much like that of the artifact associated with iontophoresis It is a 5 volt TTL signal controlled by channel one The time mark not only provides a useful indication of the duration of the pulse but it may also serve as a sync out signal for triggering the sweep of an oscilloscope etc HOLDERS FOR PRESSURE PIPETTES Standard pipette holders...

Страница 7: ...ars as precursors of glycoproteins 12 in order to study neuron specific transmitter biosynthesis axonal transport and cellular topography The reproducible and quantifiable ejections obtained with pressure systems make them ideal for neuropharmacological studies of agonist and drug interactions with membrane receptors 1 3 4 As you find additional uses for your Picospritzer please send us a reprint ...

Страница 8: ...tylcholine by identified neurons of Aplysia Proc Nat Acad Sci 70 3371 1973 12 Thompson E B Schwartz J H and Kandel E R A radio autographic analysis in the light and electron microscope of identified Aplysia neurons and their processes after intrasomatic injection of L H3 Fucose Brain Research 112 251 1976 13 Baux G Simonneau M Tauc L Transmitter release Ruthenium red used to demonstrate a possible...

Страница 9: ...d FIGURE 2A LINEARITY OF VOLUME EJECTED WITH VAYRING PULSE DURATION AT CONSTANT PRESSURE The X intercept 15 MSEC represents the mechanical lag time of the particular solenoid valve FIGURE 2B LINEARITY OF VOLUME EJECTED WITH VARYING PRESSURE AT CONSTANT PULSE DURATION The X intercept 7 5PSI represents the minimum pressure necessary for ejection and is a characteristic of a particular pipette FIGURE...

Страница 10: ...onse standard error of the mean Problems of desensitization can be circumvented with a pressure pipette since the pipette is usually filled with agonists in the concentrations of 10 6 to 10 3 M in contrast to iontophoretic pipettes Braking current is not necessary for the pressure pipette thus avoiding inconsistent ejection of compounds FIGURE 3C Reproducibility of 12 consecutive responses to acet...

Страница 11: ...ch OD tubing 3 feet long nut ferrule on both ends REPLACEMENT GASKETS P N 050 00XX GSK KIT XX should be replaced by your Pipette s OD Example P N 050 0010 GSK KIT is for a 1 0 mm pipette holder Each Kit comes with 10 replacement gaskets and 1 replacement nut PICOSPRITZER ACCESSORIES Pushbutton Cable Assembly P N 050 0000 800 1 Footswitch Cable Assembly P N 050 0000 801 1 PIPETTE HOLDERS P N 050 00...

Страница 12: ...the tubing required to connect it to the front panel mounted regulator assembly and to a pipette holder 12 feet of inch OD tubing are supplied to make the connection between the regulator assembly and the compressed gas supply maximum 150 psig Expansion capability for a second valve box is built in B Two Channel This PICOSPRITZER uses the same electrical chassis as the single channel but includes ...

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