http://www.parker.com/ppf
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Contents
SAFETY.................................................... 2
EQUIPMENT
SPECIFICATIONS................................... 2
INTRODUCTION..................................... 3
OPERATING SPECIFICATIONS............ 4
CONTROLS AND USE............................ 4
BACKGROUND.......................................7
PICOSPRITZER® III MODELS..............11
PICOSPRTIZER® III
Pressure Systems for Ejection of
Picoliter Volumes in Cell Research
OPERATING MANUAL
I. INTRODUCTION
Please read this entire set of instructions before
attempting to use this instrument for
intracellular or extracellular ejections. These
instructions cover all currently produced systems
(single channel and two channel models).
The Picospritzer III is a self-contained, rack-
mountable system which supplies repeatable pressure
pulses. Volumes dispensed are linear with time and
pressure. The system can be initiated three ways:
front panel push button, external stimulator (5 volt),
or remote push button/foot pedal. The Picospritzer III
pressure system was designed for rapid and
reproducible ejections of picoliter to nanoliter
volumes used in conjunction with intracellular or
extracellular studies while avoiding the inherent
desensitization of nerve cells which accompanies
Iontophoretic methodology.
Intracellular applications range from femtoliter
ejections of RNA into small (50m diameter) cells to
nanoliter ejections into oocytes. Extracellular
applications range from picoliter applications of
dilute neuroactive substances onto neurons during
intracellular recording to the presentation of chemical
stimuli to whole animals.
The system comes complete with high speed valve(s)
and the necessary tubing assemblies (less pipette and
holder). It is designed to fit a standard 19" relay rack.
The Picospritzer III comes fully adjusted and ready to
use.
