Parker ChemTec II, Инструкции по установке, эксплуатации и обслуживанию

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Linear, Volumetric Bioreactor Feed
A Parker customer provided the following data: A ChemTec II was used to automate nutrient feed into a
bioreactor. During the initial growth phase, a linear nutrient ramp was implemented that lasted four hours,
followed by a very slow ramp during the remaining eleven hours:
Time,
hrs.
Rate,
ml/min
Time,
hrs.
Rate,
ml/min
Time,
hrs.
Rate,
ml/min
0.0 35.60 5.0 96.62 10.5 97.37
0.5 43.21 5.5 96.69 11.0 97.43
1.0 50.66 6.0 96.75 11.5 97.50
1.5 57.83 6.5 96.83 12.0 97.56
2.0 66.04 7.0 96.89 12.5 97.65
2.5 73.65 7.5 96.96 13.0 97.65
3.0 81.26 8.0 97.02 13.5 97.65
3.5 88.87 9.0 97.16 14.0 97.65
4.0 96.48 9.5 97.23 14.5 97.65
4.5 96.55 10.0 97.29 15.0 97.65
A ChemTec II CP-120 with a TANDEM 1081 peristaltic pump head (160 RPM pump motor) was used for this
feed application. PharMed pump tubing #16 was chosen since it can generate flow rates from 4.6 ml/min. to
120 ml/min.
The following Recipe was entered into the Volu Flow mode:
START
Interpolate from 35.6 ml/min to 96.48 ml/min over 4 hours
Interpolate from 96.48 ml/min to 97.65 ml/min over 11 hours
END
When executing the above VOLU FLOW program, the ChemTec II will generate an initial pump rate of 35.60
ml/min, which is ramped up to 96.48 ml/min. over a four-hour period. This initial ramp is followed by an
almost constant pump rate, when actually; the pump rate of 96.48 ml/min. is increased to 97.65 ml/min. over
an 11-hour period. During the entire 15-hour operation of the ChemTec II, no human intervention or
supervision was required. The ChemTec II performance data was printed out at 15-minute intervals.
0.00
20.00
40.00
60.00
80.00
100.00
120.00
0.0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0
Pump
R
ate,
m
l/min.
Time, hrs
Figure 1
Pump Rate vs Time