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19
Start-up: Mass Flow Metering into a Bioreactor
EQUIPMENT
You will need the following items to get started:
Parker P/N
Description
Quantity
CHEM2-21
ChemTec II, CP-120 w/ 1081 Tandem Peristaltic Head, 160rpm Motor
1 pc
100-VIPER6
Mettler Toledo ICS425 Scale, 6000gm x.1gm
1 pc
400-116
Silicone Tubing, Platinum Cured, #16
25 ft (1 pkg)
080-072ICS
Cable, Interfaces ChemTec II and Mettler Scale
1pc
080-USB
RS232 to USB Adapter Cable
1 kit
N/A
Appropriate Media Reservoir
1 pc
N/A
Appropriate Bioreactor or Fermentor
1 pc
Hardware Setup
1.
Unpack all the components, visually identify and inspect for damage.
2.
At the bench, place the scale with the media reservoir to the far left. To the right of the bench, position
the ChemTec II and Bioreactor. Leave space between these items to allow for cables and tubing.
3.
Connect the interface cable between the ChemTec II and the scale, connecting it to the “S1” connector on
the rear panel of the ChemTec II.
4.
Connect the RS232 to USB cable between the PC and the “Printer” connector on the rear of the ChemTec
II. This cable may require a driver installation from the internet, and assistance from your internal IT
department to download.
5.
Plug in to power equipment.
6.
Install SciDoc II software from
https://discover.parker.com/pump-support-gen2
. Choose ChemTec II
from the menu, select the appropriate COM Port, and it will be ready to receive data from the pump.
7.
If the ChemTec II and scale were purchased together from Parker, both will be configured to
communicate with each other. If purchased separately, contact Parker Technical Support
(
for assistance.
8.
Cut an appropriate length of #16 tubing and connect it to the Media reservoir. Route the tubing from the
reservoir to the ChemTec II, open the head by rotating the lever 180 degrees counter-clockwise, and
place the tubing over the upper set of rollers. Confirm that the tubing is under the centering springs and
close the head by rotating the lever back to its original position. Connect the remaining end to the
Bioreactor.
This completes the hardware and tubing configuration.
PROGRAM EDITING AND EXECUTION
At this point, consider the parameters of the reaction being fed and determine the type of metering needed.
This can be done at a constant rate, a linear rate, or an exponential rate by using several short linear ramps.
Several user-definable alarms may be utilized to monitor the process; solution or reactor weights if balances
are connected to monitor them and the measured pressure for one to three of the connected pressures. All
the alarms may be disabled or enabled and set to interlock and provide and audible alarm.
Содержание ChemTec II
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