20
Table 7
Total System Hold-up Volume Calculation Chart
Total Volume Added
A*
1
___________ mL
(A*)
Remaining Volume In
Graduated Cylinder
D
– ___________ mL
(D)
Total System Hold-up
Volume (feed/retentate/
permeate)
(A* – D)
= ___________ mL
(E)
Feed/Retentate Hold-up
Volume from Table 6
C
– ___________ mL
(C)
Permeate Hold-up
Volume
(E – C)
= ___________ mL
(F)
1
A* = initial volume of water added (A from Table 6) plus any additional volume added from Section 3.3.2 in
Step 1.
3.3.3
Determining the Non-recoverable Volume
Procedure
1. If the liquid was initially poured into the feed tank, close the permeate valve, then
put the retentate line into the graduated cylinder and slowly pump out the
remaining water in the system (combining with the volume collected from the
previous step), allowing air to purge out the lines. If a graduated cylinder was
initially used as the feed tank, close the permeate valve, then carefully remove the
feed line leaving the retentate and permeate lines in the cylinder and slowly pump
out the remaining water in the system, allowing air to purge out the lines.
2. Carefully remove the permeate line from the graduated cylinder.
3. Hold the end up and fill the line with water from the cylinder.
4. Record the total volume in the graduated cylinder (G).
5. Subtract the volume in the graduated cylinder (G) plus the permeate hold-up
volume (F) from the total volume added (A*).
The difference between the total volume added and the volume in the graduated
cylinder plus the permeate hold-up volume is the Non-recoverable Volume (X),
(Table 8).
Table 8
Non-recoverable Volume Calculation Chart
Total Volume Added
A*
2
___________ mL
(A*)
Volume in Graduated
Cylinder
G
– ___________ mL
(G)
Permeate Hold-up Volume F
+ ___________ mL
(F)
Non-recoverable Volume,
Feed/Retentate
A*– (G + F)
= ___________ mL
(X)
2
A* = initial volume of water added (A from Table 6) plus any additional volume added from Section 3.3.2 in
Step 1.
3.3.4
Determining the System Minimum Working Volume
Note: For this measurement, the actual feed tank must be used. This procedure may
be performed after the cassettes have been sanitized.
