18
4-4
Illumination device
4-4-1 Use color filter
◎
Use color filter to increase the accuracy of the
observation and photomicrography. Suggest user
to adopt LBD color filter to get more neutral hue
when observing bright-field and taking photo.
◎
Color filters could overlay on the filter holder,
make sure lay them level, and the maximum of the
thickness is 11mm.
★
Place the color filter according to fig.11 and the
note.
Color filter
Purpose
IF550
Monochromatic contrast color filter
(green)
(use in phase-contrast observation)
LBD
Color temperature transition color filter
(use in bright-field observation and
photomicrograph)
SIF800
De-infrared color filter
(use in photomicrograph to compensate
the exposure time)
4-4-2 Use the adjusting ring of aperture
diaphragm (See fig.25)
◎
The aperture diaphragm decides the numerical
aperture of the illumination when doing bright-field
observation. If the N.A. of illumination is matching
with N.A. of the objective; you can obtain better
resolution and the contrast and increase the depth of
field.
●
The objective could be moved if needed when
affirming the aperture diaphragm. (It could be
insert the centering telescope) Observing through
the eye tube, and adjust ring of aperture
diaphragm until see the view-field as
fig.25.
(Image of aperture diaphragm
①
, Objective
outline
②
)
●
Usually, adjust the N.A. to its 70%~80% when
observing the dyed specimen. Adjust the aperture
diaphragm to “ ” when observing the bacteria
specimen.
Fig 25
