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27

8. 

Trouble Shooting

   

                                                   

                             

 

Problem 

Cause 

Remedy 

Reference 

page   

Optics 

1. The bulb lightening 

but it is dark in 
viewing field. 

Lamp  base  is  not  connect  to 
illumination device 

Connect it correctly and firmly 

The bulb is burn out   

Replace it with new one 

The  light  adjust  button  turned  to 
dark end   

Adjust to proper brightness 

10 

Overlay too many color filter 

Minimize the pieces 

14 

Not use the specific bulb   

Lamp  selected  only:  6V/30W 
HAL bulb (Philips 5761)   

2. The side of the 

field of the view is 
dark or not even. 

The  nosepiece  is  not  in  the  right 
position 

Turn the nosepiece into the right 
position until hearing a click   

The  color  filter  and  its  holder 
placed incorrectly 

Place them correctly   

The  phase-contrast  slider  placed 
incorrectly 

Shift  the  slider  until  hearing  a 
click.   

17 

3.

 

Stain or dust is 

observed in the 
field of view. 

Stains  have  accumulated  on  the 
specimen 

Change specimen 

 

Stains  have  accumulated  on  the 
eyepieces 

Clean the eyepieces 

 

4. About resolution   

 

Unclear image 

  phase-contrast is 

out of work 

 

Unclear  Image  of 

fine structure 

Objectives  is  not  place  in  the 
light path   

Turn the nosepiece into the right 
position until hearing a click 

The aperture opened incorrectly   

Adjust the iris diaphragm 

14 

Stain or dust has accumulated on 
the condenser, objective, 
eyepieces, base lens 

Clean the lens 

 

The  thickness  of  specimen  slide 
or Petri dish is not 1.5mm 

Use ones with thickness of 
1.5mm   

11 

Use bright-field objective 

Change  it  with  phase-contrast 
objectives 

16 

The  light  loop  of  phase-contrast 
slider  is  unmatched  with  phase 
ring   

Use light loop matched with 
phase ring   

16 

The light loop and the phase ring 
are not centered   

Center them correctly when 
using XDSL   

17 

The light loop and the phase ring 
are  deviate  when  observing  the 
edge of the Petri dish   

Move  the  Petri  dish  to  get  the 
best phase-contrast effect   

 

5. Some parts of 

image is not in the 
focal plane   

Objectives  is  not  place  in  the 
light path 

Turn the nosepiece into the right 
position until hearing a click 

The  specimen  placed  on  the 
stage incorrectly 

Place the specimen correctly   

11 

The  optical  effect  of  the  Petri 
dish  is  not  good  (ex:  bottom 
smooth) 

Use the dish with smooth surface 

 

Содержание A14.0901-A

Страница 1: ...To ensure the safety and obtain optimum performance and to familiarize yourself fully with use of this microscope we recommend that you study this manual thoroughly before operating the microscope Ret...

Страница 2: ...g Device 10 4 1 Stand 10 4 2 Stage 11 4 3 Observing Tube 12 4 4 Illumination device 14 5 Phase contrast Observation 16 5 1 Nomenclature 16 5 2Assembling and operating 17 6 TV device Photography device...

Страница 3: ...fuse It would be better to do so after cooling the bulb and the lamp holder Lamp selected only 6V 30W HAL bulb Philips 5761 7 It can adapt wide voltage from 90 to 240V There is no need for transformer...

Страница 4: ...ty Sign Sign Signification It shows the surface gets hot and don t touch with bare hand Read the introduction before use Unsuitable operation would lead to person hurt or instrument faulty Main switch...

Страница 5: ...LWDPH40 Long work distance condenser XDCD0 3 Phase contrast slider Phase center presetting slider XDSLP Phase center adjustable slider XDSL Circular central stage Metal stage XDCPM Glass stage XDCPG S...

Страница 6: ...Stage fixed Stage extension board XDSGEX Attachable mechanical stage XDSGM Nosepiece fixed Quintuple nosepiece Illumination holder fixed Lamp holder XDAL2 Phase contrast slider Phase center presetting...

Страница 7: ...part or touch the glass face Preserve the hexagon spanner and it will be used when changing the parts Halogen Bulb 6V 30W HAL Lamp holder XDAL2 Color Transmissive illumination group Color Eyepiece PL1...

Страница 8: ...main power make sure the power switch is disconnect the power wire and wait until the lamp holder and bulb cool down 2 2 2 Assemble the transmission illumination groupware See fig 4 1 Unbolt the screw...

Страница 9: ...us the sample by low magnification objective 4X or 10X when using the equipment Then change to the high magnification ones according as the situation observation requires Turn the condenser when chang...

Страница 10: ...Insert the eyepiece into the tube thoroughly 2 2 8 Assemble the color filter Fig 11 Make sure the color filter is completely cool down before changing it Take down the filter holder and put in the co...

Страница 11: ...to the supply socket and make sure the connection is well See fig 14 Use the special wire supplied by our company Choose another suitable wire if it s lost or damaged 90 240V wide voltage Connect the...

Страница 12: ...ocation Phase contrast slide adopt phase center adjustable slide configured with phase center presetting rod Phase contract slider Color filter holder Diopter adjusting ring Interpupillary distance po...

Страница 13: ...XD20 for their location Phase contract slider Color filter holder Phase center adjusting rod The adjusting ring of aperture diaphragm Interpupillary distance pointer Diopter adjusting ring Light path...

Страница 14: ...nsion adjusting ring Make sure the coarse tension adjusting ring is being used to adjust the tension of coarse adjustment knob How to adjust tension Turn the coarse tension adjusting ring Coarse adjus...

Страница 15: ...e when using 96 or 24 microtitration plate 2 In order to clip board of any type tightly please use holder and attachable mechanical stage as follows Use Terasaki holder which to place Terasaki board U...

Страница 16: ...justment ring if the image is unclear when observing the left eyepiece with left eye See fig 21 The user could select any side of the eyepieces as a standard of reference if there has diopter adjustme...

Страница 17: ...r observation tube Shift the optical way by moving the block of shifting the optical way Shift the block to the right until hearing a click when doing normal observation Shift the block to the left un...

Страница 18: ...0 De infrared color filter use in photomicrograph to compensate the exposure time 4 4 2 Use the adjusting ring of aperture diaphragm See fig 25 The aperture diaphragm decides the numerical aperture of...

Страница 19: ...26 Turn the parts below the condenser in clockwise to get larger operation space It could get the height of 150mm Remember it would not get well illumination when operating It only proper to use big P...

Страница 20: ...ment 10X 20X light loop for 10X 20X Phase contrast objective 40X light loop for 40X Phase contrast objective hole for 45mm color filter See fig 27 5 1 3 XDSL phase contrast slider See fig 28 Phase cen...

Страница 21: ...hout diopter adjustment 3 Make sure the matched phase loop in the phase contrast objective and the light loop in the phase contrast slider is shifting into the optical way 4 While observe into the cen...

Страница 22: ...t bright image when seeing double light loop Light loop will deviate away from phase loop after moving or replacing a piece of thick specimen Repeat step 1 5 if it happens Repeat the centering steps i...

Страница 23: ...on the trinocular head and get down the dust cover 2 Get down the dust cover caps of the TV adapter Insert adapter into the trinocular as the figure shows Screw on the lock screw tightly 3 Loosen the...

Страница 24: ...he adapter to the joint of the assembly parts of camera Screw it tightly in clockwise See fig 36 5 Insert the photography eyepiece 3 2X to the photography tube then mount adapter and assembly parts of...

Страница 25: ...by view finder when taking photo See instructions of the camera assembly parts for details 6 2 4 Adjust color temperature Use daylight type film to take photo 1 Place LBD color temperature transition...

Страница 26: ...sion board 70 W 80 L mm attachable mechanical stage Work distance 120 X 80 Y mm Low coaxial focus adjustor attached with Petri dish holder Illumination Adopt 90 240V wide voltage the 6V 30W Halogen bu...

Страница 27: ...ge phase contrast is out of work Unclear Image of fine structure Objectives is not place in the light path Turn the nosepiece into the right position until hearing a click 5 The aperture opened incorr...

Страница 28: ...ot work No power supply Check the connection of the power cable 7 The bulb is not inserted correctly Insert it correctly 4 The bulb burnt out Replace it 4 2 The bulb burnt out usually Not use the spec...

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