27
8.
Trouble Shooting
Problem
Cause
Remedy
Reference
page
Optics
1. The bulb lightening
but it is dark in
viewing field.
Lamp base is not connect to
illumination device
Connect it correctly and firmly
4
The bulb is burn out
Replace it with new one
4
The light adjust button turned to
dark end
Adjust to proper brightness
10
Overlay too many color filter
Minimize the pieces
14
Not use the specific bulb
Lamp selected only: 6V/30W
HAL bulb (Philips 5761)
4
2. The side of the
field of the view is
dark or not even.
The nosepiece is not in the right
position
Turn the nosepiece into the right
position until hearing a click
5
The color filter and its holder
placed incorrectly
Place them correctly
6
The phase-contrast slider placed
incorrectly
Shift the slider until hearing a
click.
17
3.
Stain or dust is
observed in the
field of view.
Stains have accumulated on the
specimen
Change specimen
—
Stains have accumulated on the
eyepieces
Clean the eyepieces
—
4. About resolution
●
Unclear image
●
phase-contrast is
out of work
●
Unclear Image of
fine structure
Objectives is not place in the
light path
Turn the nosepiece into the right
position until hearing a click
5
The aperture opened incorrectly
Adjust the iris diaphragm
14
Stain or dust has accumulated on
the condenser, objective,
eyepieces, base lens
Clean the lens
—
The thickness of specimen slide
or Petri dish is not 1.5mm
Use ones with thickness of
1.5mm
11
Use bright-field objective
Change it with phase-contrast
objectives
16
The light loop of phase-contrast
slider is unmatched with phase
ring
Use light loop matched with
phase ring
16
The light loop and the phase ring
are not centered
Center them correctly when
using XDSL
17
The light loop and the phase ring
are deviate when observing the
edge of the Petri dish
Move the Petri dish to get the
best phase-contrast effect
—
5. Some parts of
image is not in the
focal plane
Objectives is not place in the
light path
Turn the nosepiece into the right
position until hearing a click
5
The specimen placed on the
stage incorrectly
Place the specimen correctly
11
The optical effect of the Petri
dish is not good (ex: bottom
smooth)
Use the dish with smooth surface
—